Abstract：
:High amphiphilicity is a hallmark of interfacial helices in membrane proteins and membrane-active peptides, such as toxins and antimicrobial peptides. Although there is general agreement that amphiphilicity is important for membrane-interface binding, an unanswered question is its importance relative to simple hydrophobicity-driven partitioning. We have examined this fundamental question using measurements of the interfacial partitioning of a family of 17-residue amidated-acetylated peptides into both neutral and anionic lipid vesicles. Composed only of Ala, Leu, and Gln residues, the amino acid sequences of the peptides were varied to change peptide amphiphilicity without changing total hydrophobicity. We found that peptide helicity in water and interface increased linearly with hydrophobic moment, as did the favorable peptide partitioning free energy. This observation provides simple tools for designing amphipathic helical peptides. Finally, our results show that helical amphiphilicity is far more important for interfacial binding than simple hydrophobicity.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Fernández-Vidal M,Jayasinghe S,Ladokhin AS,White SHdoi
10.1016/j.jmb.2007.05.016subject
Has Abstractpub_date
2007-07-13 00:00:00pages
459-70issue
3eissn
0022-2836issn
1089-8638pii
S0022-2836(07)00626-2journal_volume
370pub_type
杂志文章abstract：:The coat protein of positive-stranded RNA viruses often contains a positively charged tail that extends toward the center of the capsid and interacts with the viral genome. Electrostatic interaction between the tail and the RNA has been postulated as a major force in virus assembly and stabilization. The goal of this ...
journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/j.jmb.2012.03.023
更新日期：2012-06-22 00:00:00
abstract：:Bovine enterovirus (BEV) strain VG-5-27 derived from an infectious cDNA clone has been crystallized as extended hexagonal plates. Virus recovered from crystals produced cytopathic effect in BHK cells. These crystals diffract X-rays from high energy synchrotron sources to beyond 2.7 A. The crystal system is monoclinic,...
journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1993.1339
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1998.1672
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：1987-04-05 00:00:00
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journal_title：Journal of molecular biology
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/j.jmb.2012.10.007
更新日期：2012-12-14 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.2001.5027
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/j.jmb.2020.02.031
更新日期：2020-03-27 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/0022-2836(91)90252-2
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1993.1358
更新日期：1993-06-20 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1993.1037
更新日期：1993-01-20 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/0022-2836(87)90194-x
更新日期：1987-06-05 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.2000.3844
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1997.1273
更新日期：1997-10-03 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/0022-2836(91)90569-r
更新日期：1991-05-20 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1993.1431
更新日期：1993-08-05 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/j.jmb.2005.04.047
更新日期：2005-07-01 00:00:00
abstract：:The gene V protein of bacteriophage f1 binds to single-stranded nucleic acids and is essential for propagation of phage f1. We tested the function of gene V protein mutants with single amino acid substitutions in two ways: by the ability of the mutant proteins to support phage growth, and by the ability of the mutant ...
journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1994.1165
更新日期：1994-02-18 00:00:00
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journal_title：Journal of molecular biology
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journal_title：Journal of molecular biology
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1997.1525
更新日期：1998-02-13 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：1996-04-12 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：2010-04-30 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：1998-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1988-11-05 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：2004-05-07 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.jmb.2010.11.049
更新日期：2011-01-28 00:00:00
