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Binding of Escherichia coli RNA polymerase to a promoter carrying mutations that stop transcription initiation.

Abstract：

:The gal P2 promoter can be inactivated by point mutations located in the -10 hexamer sequence or immediately upstream from it. Mutations at either site reduce expression in vivo and prevent the formation, in vitro, of tight complexes with RNA polymerase that give a strong footprint and can initiate transcription. However, with a mutation upstream from the -10 region, RNA polymerase could still make a specific contact with gal promoter DNA as judged by interference with cleavage by restriction enzyme SfaNI at a site within the promoter. In contrast, with a mutation in the -10 hexamer sequence, RNA polymerase could not make this contact and does not interfere with restriction by SfaNI.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Johnston F,Ponnambalam S,Busby S

doi

10.1016/0022-2836(87)90194-x

subject

Has Abstract

pub_date

1987-06-05 00:00:00

pages

745-8

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(87)90194-X

journal_volume

195

pub_type

杂志文章

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