The oligomeric distribution of SecYEG is altered by SecA and translocation ligands.

abstract：:The bacteriophage P2 late control gene ogr was cloned and precisely localized by deletion analysis in vitro. The DNA sequence of the ogr gene containing the ogr1 mutation was determined. The sequence translates into a basic protein of a molecular weight of 8300. Plasmids overproducing the ogr gene product were constru...

journal_title：Journal of molecular biology

authors： Birkeland NK,Lindquist BH

更新日期：1986-04-05 00:00:00

abstract：:By using a set of 476 ordered DNA clones (in lambda phage vector) that covers the entire chromosome of Escherichia coli K12, we have made an exhaustive survey of tRNA genes in the E. coli genome. Ultraviolet-irradiated bacteria were separately infected with each of the 476 clones and the RNA molecules produced upon in...

journal_title：Journal of molecular biology

authors： Komine Y,Adachi T,Inokuchi H,Ozeki H

更新日期：1990-04-20 00:00:00

abstract：:The anticancer drug daunomycin has been co-crystallized with the hexanucleotide duplex sequences d(TGTACA) and d(TGATCA) and single crystal X-ray diffraction studies of these two complexes have been carried out. Structure solution of the d(TGTACA) and d(TGATCA) complexes to 1.6 and 1.7 Angstrom resolution, respectivel...

journal_title：Journal of molecular biology

authors： Nunn CM,Van Meervelt L,Zhang SD,Moore MH,Kennard O

更新日期：1991-11-20 00:00:00

abstract：:Here, we used data of complete genomes to study comparatively the metabolism of different species. We built phenetic trees based on the enzymatic functions present in different parts of metabolism. Seven broad metabolic classes, comprising a total of 69 metabolic pathways, were comparatively analyzed for 27 fully sequ...

journal_title：Journal of molecular biology

authors： Aguilar D,Aviles FX,Querol E,Sternberg MJ

更新日期：2004-07-09 00:00:00

abstract：:Cataract is a lens opacification disease prevalent worldwide. Cataract-causing mutations in crystallins generally lead to the formation of light-scattering particles in the lens. However, it remains unclear for the detailed structural and pathological mechanisms of most mutations. In this study, we showed that the G12...

journal_title：Journal of molecular biology

authors： Xi YB,Chen XJ,Zhao WJ,Yan YB

更新日期：2015-08-28 00:00:00

abstract：:Protein-primed DNA polymerases form a subgroup of the eukaryotic-type DNA polymerases family, also called family B or alpha-like. A multiple amino acid sequence alignment of this subgroup of DNA polymerases led to the identification of two insertions, TPR-1 and TPR-2, in the polymerisation domain. We showed previously...

journal_title：Journal of molecular biology

authors： Dufour E,Rodríguez I,Lázaro JM,de Vega M,Salas M

更新日期：2003-08-22 00:00:00

abstract：:Many essential biological processes are mediated by complex molecular machines comprising multiple subunits. Knowledge on the architecture of individual subunits and their positions within the overall multimeric complex is key to understanding the molecular mechanisms of macromolecular assemblies. The anaphase-promoti...

journal_title：Journal of molecular biology

authors： Cronin NB,Yang J,Zhang Z,Kulkarni K,Chang L,Yamano H,Barford D

更新日期：2015-10-09 00:00:00

abstract：:The rat Clara cell 17 kDa protein (previously referred to as the rat Clara cell 10 kDa protein) has been reported to inhibit phospholipase A2 and papain, and to also bind progesterone. It has been isolated from rat lung lavage fluid and crystallized in the space group P6(5)22. The structure has been determined to 3.0 ...

journal_title：Journal of molecular biology

authors： Umland TC,Swaminathan S,Furey W,Singh G,Pletcher J,Sax M

更新日期：1992-03-20 00:00:00

abstract：:The chicken nuclear lamina is composed of at least three proteins called lamins A, B1 and B2. In addition, putative precursors are transiently expressed during in vivo synthesis of lamins A and B2. Here we report the complete sequence of lamin B2 as it is deduced from a cloned cDNA. Comparison of lamin B2 with lamins ...

journal_title：Journal of molecular biology

authors： Vorburger K,Lehner CF,Kitten GT,Eppenberger HM,Nigg EA

更新日期：1989-08-05 00:00:00

abstract：:Antidote/toxin gene pairs known as "addiction modules" can maintain plasmids in bacterial populations by means of post-segregational killing. However, several chromosome-encoded addiction modules may provide an entirely distinct function in the programmed cell death of moribund subpopulations under starvation conditio...

journal_title：Journal of molecular biology

authors： Bishop RE,Leskiw BK,Hodges RS,Kay CM,Weiner JH

更新日期：1998-07-24 00:00:00

abstract：:To characterize driving forces and driven processes in formation of a large-interface, wrapped protein-DNA complex analogous to the nucleosome, we have investigated the thermodynamics of binding the 34-base pair (bp) H' DNA sequence to the Escherichia coli DNA-remodeling protein integration host factor (IHF). Isotherm...

journal_title：Journal of molecular biology

authors： Vander Meulen KA,Saecker RM,Record MT Jr

更新日期：2008-03-14 00:00:00

abstract：:The structure of the complex between a recombinant single-chain Fv construct of antibody NC10 with a five-residue peptide linker between VH and VL (termed scFv(5)), and its antigen, tetrameric neuraminidase from influenza virus (NA), has been determined and refined at 2.5 A resolution. The antibody-antigen binding int...

journal_title：Journal of molecular biology

authors： Malby RL,McCoy AJ,Kortt AA,Hudson PJ,Colman PM

更新日期：1998-06-19 00:00:00

abstract：:The cytokine interleukin 13 (IL-13) is a major effector molecule for T-helper type 2 inflammation and is pathogenic in allergic diseases such as asthma. The effects of IL-13 are mediated via a pathway that is initiated by binding to a heterodimeric receptor consisting of IL-13Rα1 and IL-4Rα. Antibodies raised against ...

journal_title：Journal of molecular biology

authors： Ultsch M,Bevers J,Nakamura G,Vandlen R,Kelley RF,Wu LC,Eigenbrot C

更新日期：2013-04-26 00:00:00

abstract：:Muscular dystrophy with myositis (mdm) is a recessive mouse mutation that is caused by a small deletion in the giant elastic muscle protein titin. Homozygous mdm/mdm mice develop a progressive muscular dystrophy, leading to death at approximately 2 months of age. We surveyed the transcriptomes of skeletal muscles from...

journal_title：Journal of molecular biology

authors： Witt CC,Ono Y,Puschmann E,McNabb M,Wu Y,Gotthardt M,Witt SH,Haak M,Labeit D,Gregorio CC,Sorimachi H,Granzier H,Labeit S

更新日期：2004-02-06 00:00:00

abstract：:Neutron-scattering in combination with quasi-elastic light-scattering and electron microscopy was used to derive a model for the capsid structure of the Top a-t component of alfalfa mosaic virus (AMV-Ta-t). In the electron microscope, AMV-Ta-t appears as an irregular ellipsoidal particle with apparent dimensions 275 (...

journal_title：Journal of molecular biology

authors： Cusack S,Oostergetel GT,Krijgsman PC,Mellema JE

更新日期：1983-12-05 00:00:00

abstract：:We produced human leukotriene B(4) (LTB(4)) receptor BLT1 as a recombinant protein in Escherichia coli. This detergent-solubilized receptor displays two states with regard to its affinity for LTB(4): (i) a low-affinity state (K(a)=7.8x10(8)M(-1)) that involves a receptor homodimer (BLT1.LTB(4))(2); we report evidence ...

journal_title：Journal of molecular biology

authors： Banères JL,Parello J

更新日期：2003-06-13 00:00:00

abstract：:The chemistry of active-site cysteine residues is central to the activity of thiol-disulfide oxidoreductases of the thioredoxin superfamily. In these reactions, a nucleophilic thiolate is required, but the associated pK(a) values differ vastly in the superfamily, from less than 4 in DsbA to greater than 7 in Trx. The ...

journal_title：Journal of molecular biology

authors： Foloppe N,Sagemark J,Nordstrand K,Berndt KD,Nilsson L

更新日期：2001-07-06 00:00:00

abstract：:A complete understanding of antibody-antigen association and specificity requires the stereochemical description of both antigen and antibody before and upon complex formation. The structural mechanism involved in the binding of the IgG1 monoclonal antibody E8 to its highly charged protein antigen horse cytochrome c (...

journal_title：Journal of molecular biology

authors： Mylvaganam SE,Paterson Y,Getzoff ED

更新日期：1998-08-14 00:00:00

abstract：:We have used two structurally well-characterized serine proteinase variants, subtilisins Carlsberg and BPN', to produce (Cys)-S-/(His)-Im+H ion-pairs by chemical mutation in well defined, different, electrostatic microenvironments. These ion-pairs have been characterized by pH-dependent rapid reaction kinetics using, ...

journal_title：Journal of molecular biology

authors： Plou FJ,Kowlessur D,Malthouse JP,Mellor GW,Hartshorn MJ,Pinitglang S,Patel H,Topham CM,Thomas EW,Verma C,Brocklehurst K

更新日期：1996-04-19 00:00:00

abstract：:A mutant of bovine pancreatic trypsin inhibitor (BPTI) has been constructed and expressed in Escherichia coli in order to probe the kinetic and structural consequences of truncating the binding loop residues to alanine. In addition to two such mutations (Thr11Ala and Pro13Ala), it has a conservative Lys15Arg substitut...

journal_title：Journal of molecular biology

authors： Czapinska H,Otlewski J,Krzywda S,Sheldrick GM,Jaskólski M

更新日期：2000-02-04 00:00:00

abstract：:Bacteriophage with double-stranded, linear DNA genomes package DNA into pre-assembled icosahedral procapsids through a unique vertex. The packaging vertex contains an oligomeric ring of a portal protein that serves as a recognition site for the packaging enzymes, a conduit for DNA translocation, and the site of tail a...

journal_title：Journal of molecular biology

authors： Moore SD,Prevelige PE Jr

更新日期：2002-02-01 00:00:00

abstract：:A mannose-specific agglutinin, isolated from garlic bulbs, has been crystallized in the presence of a large excess of alpha-d-mannose, in space group C2 and cell dimensions, a=203.24, b=43.78, c=79.27 A, beta=112.4 degrees, with two dimers in the asymmetric unit. X-ray diffraction data were collected up to a nominal r...

journal_title：Journal of molecular biology

authors： Chandra NR,Ramachandraiah G,Bachhawat K,Dam TK,Surolia A,Vijayan M

更新日期：1999-01-22 00:00:00

abstract：:The most severe form of human malaria is caused by the protozoan parasite Plasmodium falciparum. The primary antigenic and virulence determinant expressed on the surface of infected red blood cells is PfEMP1 (P. falciparum erythrocyte membrane protein 1), a protein that mediates adhesion and sequestration of the paras...

journal_title：Journal of molecular biology

authors： Dzikowski R,Deitsch KW

更新日期：2008-10-03 00:00:00

abstract：:The presence of certain local structural elements in superhelical DNA, such as cruciforms and denatured loops, complicates the topological and geometric analysis of these molecules. In particular, the duplex axis is often difficult to define. In consequence, the usual conservation condition, Lk = Tw + Wr, is often ina...

journal_title：Journal of molecular biology

authors： White JH,Bauer WR

更新日期：1987-05-05 00:00:00

abstract：:Guanine-uracil (G.U) wobble base-pairs are a detrimental lesion in DNA. Previous investigations have shown that such wobble base-pairs are more prone to base-opening than the normal G.C base-pairs. To investigate the sequence-dependence of base-pair opening we have performed 5ns molecular dynamics simulations on G.U w...

journal_title：Journal of molecular biology

authors： Seibert E,Ross JB,Osman R

更新日期：2003-07-18 00:00:00

abstract：:We have used fluorescence resonance energy transfer to investigate the conformation of the apo and calcium-loaded states of the regulatory N-terminal domain of full-length troponin C mutants from skeletal muscle. The mutants studied each contained a single tryptophan residue (position 22 or 90) and a single cysteine r...

journal_title：Journal of molecular biology

authors： She M,Xing J,Dong WJ,Umeda PK,Cheung HC

更新日期：1998-08-21 00:00:00

abstract：:As regulators of ubiquitous biological processes, serine proteases can cause disease states when inappropriately expressed or regulated, and are thus rational targets for inhibition by drugs. Recently we described a new inhibition mechanism applicable for the development of potent, selective small molecule serine prot...

journal_title：Journal of molecular biology

authors： Katz BA,Luong C

更新日期：1999-09-24 00:00:00

abstract：:We have previously shown that transcription of the Xenopus U6 snRNA gene by RNA polymerase III is stimulated in injected Xenopus oocytes by an activator element termed the DSE, which contains an octamer sequence. Data presented here reveal that the DSE contains, in addition, a GC-rich sequence capable of binding Sp1. ...

journal_title：Journal of molecular biology

authors： Lescure A,Tebb G,Mattaj IW,Krol A,Carbon P

更新日期：1992-11-20 00:00:00

abstract：:A gene encoding a major entomocidal polypeptide from Bacillus thuringiensis subspecies kyushuensis delta-endotoxin crystals (CytB) was cloned into Escherichia coli and sequenced. The deduced amino acid sequence gave a predicted molecular mass of 29,236 Da and showed 39% identity and 70% similarity with the 27,371 Da C...

journal_title：Journal of molecular biology

authors： Koni PA,Ellar DJ

更新日期：1993-01-20 00:00:00

abstract：:The non-steroidal anti-estrogen tamoxifen [TAM] has been in clinical use over the last two decades as a potent adjunct chemotherapeutic agent for treatment of breast cancer. It has also been given prophylactically to women with a strong family history of breast cancer. However, tamoxifen treatment has also been associ...

journal_title：Journal of molecular biology

authors： Shimotakahara S,Gorin A,Kolbanovskiy A,Kettani A,Hingerty BE,Amin S,Broyde S,Geacintov N,Patel DJ

更新日期：2000-09-15 00:00:00