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Structure of the Top a-t component of alfalfa mosaic virus. A non-icosahedral virion.

Abstract：

:Neutron-scattering in combination with quasi-elastic light-scattering and electron microscopy was used to derive a model for the capsid structure of the Top a-t component of alfalfa mosaic virus (AMV-Ta-t). In the electron microscope, AMV-Ta-t appears as an irregular ellipsoidal particle with apparent dimensions 275 (+/- 31) A X 225 (+/- 22) A. Assuming that the particles are monodisperse, model calculations show that the neutron-scattering data are best explained by an oblate ellipsoidal shape for the virion with external dimensions 284 A X 284 A X 216 A. Based on this result, and in combination with the known composition of the virion, it is suggested that the capsid structure could be based on a deltahedron with 52 pointgroup symmetry and comprising 120 subunits. Such a model would imply a greater deviation from equivalent subunit interactions than normally necessary in icosahedral capsids. The neutron and photon correlation data, however, do not allow us to rule out the possibility that Top a-t is a slightly polydisperse preparation of irregular prolate shapes with mean dimensions 312 A X 232 A X 232 A. Both possibilities support the concept of alfalfa mosaic virus coat protein being capable of a wide range of intersubunit interactions, this flexibility resulting in considerable polymorphism in capsid structures.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Cusack S,Oostergetel GT,Krijgsman PC,Mellema JE

doi

10.1016/s0022-2836(83)80350-7

subject

Has Abstract

pub_date

1983-12-05 00:00:00

pages

139-55

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(83)80350-7

journal_volume

171

pub_type

杂志文章

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