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A factor with Sp1 DNA-binding specificity stimulates Xenopus U6 snRNA in vivo transcription by RNA polymerase III.

Abstract：

:We have previously shown that transcription of the Xenopus U6 snRNA gene by RNA polymerase III is stimulated in injected Xenopus oocytes by an activator element termed the DSE, which contains an octamer sequence. Data presented here reveal that the DSE contains, in addition, a GC-rich sequence capable of binding Sp1. Both elements are required to obtain wild-type levels of U6 transcription in vivo. The Xenopus U6 DSE exhibits optimal activation properties only when positioned at its normal location upstream from the start site. The U6 Sp1 motif binds the mammalian Sp1 transcriptional activator independently of the Oct-1 protein in vitro. Those mutations that lead to a reduced transcription level in vivo abolish the binding of Sp1 in vitro. Thus, transcriptional stimulation through the Xenopus U6 Sp1 motif is likely to be mediated by a protein with DNA-binding specificity identical to mammalian Sp1. These findings support the notion that RNA polymerase II and III transcription complexes share transactivators.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Lescure A,Tebb G,Mattaj IW,Krol A,Carbon P

doi

10.1016/0022-2836(92)90828-8

keywords:

subject

Has Abstract

pub_date

1992-11-20 00:00:00

pages

387-94

issue

2

eissn

0022-2836

issn

1089-8638

pii

0022-2836(92)90828-8

journal_volume

228

pub_type

杂志文章

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