当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > The positive transcription elongation factor b is an essential cofactor for the activation of transcription by myocyte enhancer factor 2.

The positive transcription elongation factor b is an essential cofactor for the activation of transcription by myocyte enhancer factor 2.

Abstract：

:The positive transcription elongation factor b (P-TEFb), composed of cyclin-dependent kinase 9 and cyclin T1, stimulates the elongation of transcription by hyperphosphorylating the C-terminal region of RNA polymerase II. Aberrant activation of P-TEFb results in manifestations of cardiac hypertrophy in mice, suggesting that P-TEFb is an essential factor for cardiac myocyte function and development. Here, we present evidence that P-TEFb selectively activates transcription mediated by the myocyte enhancer factor 2 (MEF2) family of transcription factors, key regulatory factors for myocyte development. Knockdown of endogenous cyclin T1 in murine C2C12 cells abolishes MEF2-dependent reporter gene expression as well as transcription of endogenous MEF2 target genes, whereas overexpression of P-TEFb enhances MEF2-dependent transcription. P-TEFb interacts with MEF2 both in vitro and in vivo. Activation of MEF2-dependent transcription induced by serum starvation is mediated by a rapid dissociation of P-TEFb from its inhibitory subunit, HEXIM1, and a subsequent recruitment of P-TEFb to MEF2 binding sites in the promoter region of MEF2 target genes. These results indicate that recruitment of P-TEFb is a critical step for stimulation of MEF2-dependent transcription, therefore providing a fundamentally important regulatory mechanism underlying the transcriptional program in muscle cells.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Nojima M,Huang Y,Tyagi M,Kao HY,Fujinaga K

doi

10.1016/j.jmb.2008.07.017

subject

Has Abstract

pub_date

2008-10-03 00:00:00

pages

275-87

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(08)00858-9

journal_volume

382

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Single amino acid changes in the Bacillus thuringiensis var. israelensis delta-endotoxin affect the toxicity and expression of the protein.

    abstract：:Site-directed mutagenesis has been used to change individual amino acids of the larvicidal 27,000 Mr delta-endotoxin of Bacillus thuringiensis var. israelensis. Basic and acidic residues have been systematically replaced by alanine, and the resulting mutant polypeptides analysed for cytolytic and larvicidal activity, ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(88)90283-5

    authors： Ward ES,Ellar DJ,Chilcott CN

    更新日期：1988-08-05 00:00:00

  • A shortened form of the Tetrahymena thermophila group I intron can catalyze the complete splicing reaction in trans.

    abstract：:The group I intron from Tetrahymena thermophila is able to catalyze its own excision from a precursor RNA. The intron recognizes the splice sites through an intron-encoded sequence called the internal guide sequence, or IGS. The 5' and 3' exons are thought to align on the IGS and form a pseudoknot structure consisting...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1541

    authors： Sargueil B,Tanner NK

    更新日期：1993-10-20 00:00:00

  • Conformational isomers of a class II MHC-peptide complex in solution.

    abstract：:A number of kinetic measurements of peptide dissociation from class II MHC-peptide complexes provide compelling evidence for the existence of conformational isomers in solution. There is evidence that T-lymphocytes can distinguish such isomers. However, virtually nothing is known about the structure of these isomers. ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2463

    authors： Schmitt L,Boniface JJ,Davis MM,McConnell HM

    更新日期：1999-02-12 00:00:00

  • Refined structure of the nicotinic acetylcholine receptor at 4A resolution.

    abstract：:We present a refined model of the membrane-associated Torpedo acetylcholine (ACh) receptor at 4A resolution. An improved experimental density map was obtained from 342 electron images of helical tubes, and the refined structure was derived to an R-factor of 36.7% (R(free) 37.9%) by standard crystallographic methods, a...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.12.031

    authors： Unwin N

    更新日期：2005-03-04 00:00:00

  • Structural studies of the enveloped dsRNA bacteriophage phi 6 of Pseudomonas syringae by Raman spectroscopy. I. The virion and its membrane envelope.

    abstract：:We report and interpret the first Raman spectrum of a double-stranded RNA virus containing a membrane envelope. Spectra of the native bacteriophage phi 6 and of its isolated host-attachment (spike) protein and phospholipid-free core assembly were collected from aqueous solutions over a wide range of temperature. Compa...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1163

    authors： Li T,Bamford DH,Bamford JK,Thomas GJ Jr

    更新日期：1993-03-20 00:00:00

  • Purification and crystallization of fumarase C from Escherichia coli.

    abstract：:Fumarase C purified from Escherichia coli has been crystallized in the presence of polyethylene glycol in both a citrate buffer at pH 5.3 and a 3-(4-morpholino)-propanesulfonic acid buffer at pH 7.5 yielding two crystal forms. An orthorhombic C222(1) form was obtained in citrate at pH 5.3 and an orthorhombic I222 form...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1264

    authors： Weaver TM,Levitt DG,Banaszak LJ

    更新日期：1993-05-05 00:00:00

  • V-shaped scaffold: a new architectural motif identified in an A x (G x G x G x G) pentad-containing dimeric DNA quadruplex involving stacked G(anti) x G(anti) x G(anti) x G(syn) tetrads.

    abstract：:We report the results of an NMR study of unlabeled and uniformly (13)C,(15)N-labeled d(G(3)AG(2)T(3)G(3)AT) in 100 mM NaCl, conditions under which it forms a dimeric quadruplex containing several new topological features. The DNA oligomer chain in each symmetry-related monomer subunit undergoes three sharp turns to fo...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.4916

    authors： Zhang N,Gorin A,Majumdar A,Kettani A,Chernichenko N,Skripkin E,Patel DJ

    更新日期：2001-08-31 00:00:00

  • Characterization of murine erythropoietin receptor genes.

    abstract：:We have isolated and characterized the murine genomic and complementary DNAs encoding erythropoietin (Epo) receptor from Epo-responsive and unresponsive mouse erythroleukemia cells. Two classes of Epo receptor cDNAs were isolated from Epo-responsive cells. One is a 55,000 Mr membrane-bound Epo receptor, and the other ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(90)90384-x

    authors： Kuramochi S,Ikawa Y,Todokoro K

    更新日期：1990-12-05 00:00:00

  • The importance of somatic mutations in the V(lambda) gene 2a2 in human monoclonal anti-DNA antibodies.

    abstract：:2a2 is the most commonly rearranged gene in the human V(lambda )locus. It has been postulated that certain immunoglobulin genes (including 2a2) are rearranged preferentially because their germline sequences encode structures capable of binding to a range of antigens. Somatic mutation could then increase the specificit...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4491

    authors： Rahman A,Haley J,Radway-Bright E,Nagl S,Low DG,Latchman DS,Isenberg DA

    更新日期：2001-03-16 00:00:00

  • pH-dependent interactions and the stability and folding kinetics of the N-terminal domain of L9. Electrostatic interactions are only weakly formed in the transition state for folding.

    abstract：:The role of electrostatic interactions in the stability and the folding of the N-terminal domain of the ribosomal protein L9 (NTL9) was investigated by determining the effects of varying the pH conditions. Urea denaturations and thermal unfolding experiments were used to measure the free energy of folding, DeltaG degr...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3752

    authors： Luisi DL,Raleigh DP

    更新日期：2000-06-16 00:00:00

  • Mutant alleles of the MRS2 gene of yeast nuclear DNA suppress mutations in the catalytic core of a mitochondrial group II intron.

    abstract：:Previous studies show that some yeast strains carrying point mutations of domain 5 that block splicing of a mitochondrial group II intron yield spontaneous revertants in which splicing is partially restored by dominant mutations of nuclear genes. Here we cloned and sequenced the suppressor allele of one such gene, and...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2021

    authors： Schmidt U,Maue I,Lehmann K,Belcher SM,Stahl U,Perlman PS

    更新日期：1998-09-25 00:00:00

  • Protein unfolding, and the "tuning in" of reversible intermediate states, in protic ionic liquid media.

    abstract：:Protic ionic liquids (PILs) are currently being shown to be as interesting and valuable to chemical manipulations as the well-known aprotic ionic liquids (APIL). PILs have the additional advantage that the proton activity (PA) can be adjusted by the choice of Bronsted base and Bronsted acid used in their formation. In...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.02.050

    authors： Byrne N,Angell CA

    更新日期：2008-05-02 00:00:00

  • Comparison of the human apolipoprotein genes. Apo AII presents a unique functional intron-exon junction.

    abstract：:The structure and function of the apolipoproteins are of interest because of their central role in lipid metabolism. We report the complete primary structure of the human apo AII and CIII genes. These, like apo AI, contain three introns located at conserved positions. This may reflect their evolutionary and functional...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(85)90255-4

    authors： Shelley CS,Sharpe CR,Baralle FE,Shoulders CC

    更新日期：1985-11-05 00:00:00

  • Phosphorylation of Acanthamoeba actophorin (ADF/cofilin) blocks interaction with actin without a change in atomic structure.

    abstract：:LIM-kinase activated by GST-Pak1 phosphorylates Acanthamoeba actophorin stoichiometrically and specifically on serine 1. The atomic structure of phosphorylated actophorin determined by X-ray crystallography is essentially identical with the structure of unphosphorylated actophorin. We compared biochemical properties o...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3336

    authors： Blanchoin L,Robinson RC,Choe S,Pollard TD

    更新日期：2000-01-14 00:00:00

  • The recognition of distorted DNA structures by HMG-D: a footprinting and molecular modelling study.

    abstract：:The high mobility group (HMG) domain is a DNA binding motif found in some eukaryotic chromosomal proteins and transcription factors. This domain binds in the minor groove of DNA inducing a sharp bend and also preferentially binds to certain distorted DNA structures. Although structures of sequence-specific HMG domains...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3246

    authors： Payet D,Hillisch A,Lowe N,Diekmann S,Travers A

    更新日期：1999-11-19 00:00:00

  • Influence of amino acid and peptide counterions on the conformation of DNA.

    abstract：:We describe fibre diffraction studies on the interaction of DNA with different amino acids and peptides. The B form of DNA, with ten base-pairs per turn, is always found at high levels of humidity. We suggest that this pitch is observed because the DNA molecules are maintained in a straight position. In solution, the ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(86)90444-4

    authors： Campos JL,Portugal J,Subirana JA,Mayer R

    更新日期：1986-02-05 00:00:00

  • Bacterial Signaling to the Nervous System through Toxins and Metabolites.

    abstract：:Mammalian hosts interface intimately with commensal and pathogenic bacteria. It is increasingly clear that molecular interactions between the nervous system and microbes contribute to health and disease. Both commensal and pathogenic bacteria are capable of producing molecules that act on neurons and affect essential ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2016.12.023

    authors： Yang NJ,Chiu IM

    更新日期：2017-03-10 00:00:00

  • Efficient recovery of high-affinity antibodies from a single-chain Fab yeast display library.

    abstract：:Yeast display is a powerful technology for the isolation of monoclonal antibodies (mAbs) against a target antigen. Antibody libraries have been displayed on the surface of yeast as both single-chain variable fragment (scFv) and antigen binding fragment (Fab). Here, we combine these two formats to display well-characte...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.04.019

    authors： Walker LM,Bowley DR,Burton DR

    更新日期：2009-06-05 00:00:00

  • Molecular Basis of Class III Ligand Recognition by PDZ3 in Murine Protein Tyrosine Phosphatase PTPN13.

    abstract：:Protein tyrosine phosphatase PTPN13, also known as PTP-BL in mice, represents a large multi-domain non-transmembrane scaffolding protein that contains five consecutive PDZ domains. Here, we report the solution structures of the extended murine PTPN13 PDZ3 domain in its apo form and in complex with its physiological li...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2018.08.023

    authors： Kock G,Dicks M,Yip KT,Kohl B,Pütz S,Heumann R,Erdmann KS,Stoll R

    更新日期：2018-10-19 00:00:00

  • A complex RNA-cleaving DNAzyme that can efficiently cleave a pyrimidine-pyrimidine junction.

    abstract：:Several RNA-cleaving deoxyribozymes (DNAzymes) have been reported for efficient cleavage of purine-containing junctions, but none is able to efficiently cleave pyrimidine-pyrimidine (Pyr-Pyr) junctions. We hypothesize that a stronger Pyr-Pyr cleavage activity requires larger DNAzymes with complex structures that are d...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.05.047

    authors： Lam JC,Withers JB,Li Y

    更新日期：2010-07-23 00:00:00

  • Structural and thermodynamic insights into the recognition of native proteins by anti-peptide antibodies.

    abstract：:The mechanism by which antibodies elicited against protein-derived peptides achieve cross-reactivity with their cognate proteins remains unknown. To address this question, we have carried out the complete thermodynamic characterization of the association of a monoclonal antibody (260.33.12) raised against a peptide (S...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2013.02.031

    authors： Armstrong A,Hildreth JEK,Amzel LM

    更新日期：2013-06-12 00:00:00

  • Evolution of stability in a cold-active enzyme elicits specificity relaxation and highlights substrate-related effects on temperature adaptation.

    abstract：:Molecular aspects of thermal adaptation of proteins were studied by following the co-evolution of temperature dependence, conformational stability, and substrate specificity in a cold-active lipase modified via directed evolution. We found that the evolution of kinetic stability was accompanied by a relaxation in subs...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.10.026

    authors： Gatti-Lafranconi P,Natalello A,Rehm S,Doglia SM,Pleiss J,Lotti M

    更新日期：2010-01-08 00:00:00

  • SH3-SPOT: an algorithm to predict preferred ligands to different members of the SH3 gene family.

    abstract：:We have developed a procedure to predict the peptide binding specificity of an SH3 domain from its sequence. The procedure utilizes information extracted from position-specific contacts derived from six SH3/peptide or SH3/protein complexes of known structure. The framework of SH3/peptide contacts defined on the struct...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3670

    authors： Brannetti B,Via A,Cestra G,Cesareni G,Helmer-Citterich M

    更新日期：2000-04-28 00:00:00

  • Local RNA target structure influences siRNA efficacy: systematic analysis of intentionally designed binding regions.

    abstract：:Contradictory reports in the literature have emphasised either the sequence of small interfering RNAs (siRNA) or the structure of their target molecules to be the major determinant of the efficiency of RNA interference (RNAi) approaches. In the present study, we analyse systematically the contributions of these parame...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.03.011

    authors： Schubert S,Grünweller A,Erdmann VA,Kurreck J

    更新日期：2005-05-13 00:00:00

  • Activation of the ghrelin receptor is described by a privileged collective motion: a model for constitutive and agonist-induced activation of a sub-class A G-protein coupled receptor (GPCR).

    abstract：:Three homology models of the human ghrelin receptor (GHS-R1a) have been generated from the available X-ray structures of rhodopsin (RHO model), opsin (OPS model) and beta-2 adrenergic receptor (B2 model). The latter was used as a starting point for combined molecular dynamics simulation (MDS) and full atom normal mode...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.09.051

    authors： Floquet N,M'Kadmi C,Perahia D,Gagne D,Bergé G,Marie J,Banères JL,Galleyrand JC,Fehrentz JA,Martinez J

    更新日期：2010-01-29 00:00:00

  • Genome-wide survey of prokaryotic O-protein phosphatases.

    abstract：:Complex and diverse signal transduction circuits are responsible for the efficient functioning of cellular network. Protein kinases and O-protein phosphatases are primarily responsible for propagating such stimuli within a eukaryotic cell. However, there is limited understanding of O-protein phosphatases in the prokar...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.07.004

    authors： Bhaduri A,Sowdhamini R

    更新日期：2005-09-23 00:00:00

  • An ecdysterone-responsive puff site in Drosophila contains a cluster of seven differentially regulated genes.

    abstract：:We have determined the molecular organization of an ecdysterone-responsive puff site in Drosophila melanogaster. The 71E puff site contains a tightly linked cluster of at least seven genes within a neighborhood of 10 X 10(3) base-pairs. All the genes are expressed in a tissue-specific manner in either the larval or th...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(86)80002-x

    authors： Restifo LL,Guild GM

    更新日期：1986-04-20 00:00:00

  • Variable oligomerization modes in coronavirus non-structural protein 9.

    abstract：:Non-structural protein 9 (Nsp9) of coronaviruses is believed to bind single-stranded RNA in the viral replication complex. The crystal structure of Nsp9 of human coronavirus (HCoV) 229E reveals a novel disulfide-linked homodimer, which is very different from the previously reported Nsp9 dimer of SARS coronavirus. In c...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.07.071

    authors： Ponnusamy R,Moll R,Weimar T,Mesters JR,Hilgenfeld R

    更新日期：2008-11-28 00:00:00

  • Characterization of the Borrelia burgdorferi RNase P RNA gene reveals a novel tertiary interaction.

    abstract：:Characterization of the RNase P RNA gene derived from Borrelia burgdorferi reveals covariation of the conserved nucleotides at positions corresponding to nucleotides 128 and 230 in Escherichia coli RNase P RNA (M1 RNA). Single base substitutions at either of these positions in M1 RNA resulted in a lack of complementat...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1467

    authors： Mattsson JG,Svärd SG,Kirsebom LA

    更新日期：1994-08-05 00:00:00

  • Functional sites of the Ada regulatory protein of Escherichia coli. Analysis by amino acid substitutions.

    abstract：:Specific cysteine residues at possible methyl acceptor sites of the Ada protein of Escherichia coli were converted to other amino acids by site-directed mutagenesis of the cloned ada gene of E. coli. Ada protein with the cysteine residue at 321 replaced by alanine was capable of accepting the methyl group from the met...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(88)90137-4

    authors： Takano K,Nakabeppu Y,Sekiguchi M

    更新日期：1988-05-20 00:00:00