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The recognition of distorted DNA structures by HMG-D: a footprinting and molecular modelling study.

Abstract：

:The high mobility group (HMG) domain is a DNA binding motif found in some eukaryotic chromosomal proteins and transcription factors. This domain binds in the minor groove of DNA inducing a sharp bend and also preferentially binds to certain distorted DNA structures. Although structures of sequence-specific HMG domains with their cognate double-helical DNA binding sites have been solved, the nature of the interaction of the domain with distorted DNA remains to be established. In this study we have investigated the interaction of HMG-D, a Drosophila counterpart of the vertebrate HMG1, with a DNA oligomer containing a bulge of two adenine residues. We show by footprinting that HMG-D binds preferentially on one side of the bulged DNA. Based on these data and on the published NMR structures of the HMG domain of HMG-D and the LEF-1-DNA complex, we modelled the HMG-D - bulged DNA complex. This model predicts that two residues, Val32 and Thr33, in the loop between alpha-helices I and II are inserted deep into the "hole" in the DNA formed by the two missing bases on one strand of the DNA bulge. Mutation of these residues confirmed that both are required for the efficient binding and bending of DNA by HMG-D. We discuss both the role of this loop in the recognition of distorted DNA structures by non-sequence specific HMG domain proteins and that of the basic tail in stabilising the induced DNA bend.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Payet D,Hillisch A,Lowe N,Diekmann S,Travers A

doi

10.1006/jmbi.1999.3246

keywords:

subject

Has Abstract

pub_date

1999-11-19 00:00:00

pages

79-91

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(99)93246-1

journal_volume

294

pub_type

杂志文章

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