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Influence of amino acid and peptide counterions on the conformation of DNA.

Abstract：

:We describe fibre diffraction studies on the interaction of DNA with different amino acids and peptides. The B form of DNA, with ten base-pairs per turn, is always found at high levels of humidity. We suggest that this pitch is observed because the DNA molecules are maintained in a straight position. In solution, the DNA molecules are bent and may have a larger pitch. The A form of DNA is never found upon dehydration. Instead, the B form may be either stabilized by the counterions or altered so that the number of base-pairs per helical turn decreases upon dehydration. Alteration is favoured either by small counterions that have a single charge or by large basic polypeptides and proteins. Stabilization is favoured by small counterions that have several charged groups. A third type of behaviour is found with some amino acids that contain hydrophobic groups, which destabilize the secondary structure of DNA, probably due to a modification of its intramolecular interactions. We have not detected any specific effect of amino acid side-chains, although the amino acid sequence has a clear influence on the interaction. We think that these observations are of interest in the pursuit of more detailed crystallographic studies on protein-DNA interactions.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Campos JL,Portugal J,Subirana JA,Mayer R

doi

10.1016/0022-2836(86)90444-4

subject

Has Abstract

pub_date

1986-02-05 00:00:00

pages

441-7

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(86)90444-4

journal_volume

187

pub_type

杂志文章

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