prlF and yhaV encode a new toxin-antitoxin system in Escherichia coli.

abstract：:The high-resolution structure of the N-terminal domain (NTD) of the retroviral capsid protein (CA) of Mason-Pfizer monkey virus (M-PMV), a member of the betaretrovirus family, has been determined by NMR. The M-PMV NTD CA structure is similar to the other retroviral capsid structures and is characterized by a six alpha...

journal_title：Journal of molecular biology

authors： Macek P,Chmelík J,Krízová I,Kaderávek P,Padrta P,Zídek L,Wildová M,Hadravová R,Chaloupková R,Pichová I,Ruml T,Rumlová M,Sklenár V

更新日期：2009-09-11 00:00:00

abstract：:To characterize driving forces and driven processes in formation of a large-interface, wrapped protein-DNA complex analogous to the nucleosome, we have investigated the thermodynamics of binding the 34-base pair (bp) H' DNA sequence to the Escherichia coli DNA-remodeling protein integration host factor (IHF). Isotherm...

journal_title：Journal of molecular biology

authors： Vander Meulen KA,Saecker RM,Record MT Jr

更新日期：2008-03-14 00:00:00

abstract：:Though up to 20% of the total RNA in bacterial cells is tRNA, the regulation of tRNA distribution on the genomic level remains unclear. tRNA distribution is governed by four processes: transcription, processing of precursor tRNA, degradation of precursor tRNA and degradation of mature tRNA. To elucidate the relationsh...

journal_title：Journal of molecular biology

authors： Dittmar KA,Mobley EM,Radek AJ,Pan T

更新日期：2004-03-12 00:00:00

abstract：:The structures of three crystal forms of the class mu human glutathione transferase GSTM2-2 have been determined. X-ray phase information was obtained independently from molecular replacement and from anomalous scattering by a single isomorphous derivative. One crystal form contains a single monomer in the asymmetric ...

journal_title：Journal of molecular biology

authors： Raghunathan S,Chandross RJ,Kretsinger RH,Allison TJ,Penington CJ,Rule GS

更新日期：1994-05-20 00:00:00

abstract：:Interactions among Bcl-2 family proteins are important for regulating apoptosis. Prosurvival members of the family interact with proapoptotic BH3 (Bcl-2-homology-3)-only members, inhibiting execution of cell death through the mitochondrial pathway. Structurally, this interaction is mediated by binding of the alpha-hel...

journal_title：Journal of molecular biology

authors： Dutta S,Gullá S,Chen TS,Fire E,Grant RA,Keating AE

更新日期：2010-05-21 00:00:00

abstract：:Antisense RNAs interact with their complementary target RNAs as folded structures. The formation of early binding intermediates is the most important step in determining the overall rates of stable complex formation in vitro and the efficiency of control in vivo. In the case of CopA and CopT (antisense/target RNA pair...

journal_title：Journal of molecular biology

authors： Nordgren S,Slagter-Jäger JG,Wagner GH

更新日期：2001-07-27 00:00:00

abstract：:A major problem in sequence alignments based on the standard dynamic programming method is that the optimal path does not necessarily yield the best equivalencing of residues assessed by structural or functional criteria. An algorithm is presented that finds suboptimal alignments of protein sequences by a simple modif...

journal_title：Journal of molecular biology

authors： Saqi MA,Sternberg MJ

更新日期：1991-06-20 00:00:00

abstract：:We have recently reported a novel human repetitive DNA (Sau3A family) that exists both in the chromosomes and in the extrachromosomal fraction. Several more clones that hybridized with the Sau3A family were isolated from the extrachromosomal fraction of HeLa cells. Use of these clones as probes has revealed that at le...

journal_title：Journal of molecular biology

authors： Kiyama R,Matsui H,Okumura K,Oishi M

更新日期：1987-02-20 00:00:00

abstract：:Non-structural protein 9 (Nsp9) of coronaviruses is believed to bind single-stranded RNA in the viral replication complex. The crystal structure of Nsp9 of human coronavirus (HCoV) 229E reveals a novel disulfide-linked homodimer, which is very different from the previously reported Nsp9 dimer of SARS coronavirus. In c...

journal_title：Journal of molecular biology

authors： Ponnusamy R,Moll R,Weimar T,Mesters JR,Hilgenfeld R

更新日期：2008-11-28 00:00:00

abstract：:The Src homology 3 (SH3) domain of pp60(c-src) (Src) plays dual roles in signal transduction, through stabilizing the repressed form of the Src kinase and through mediating the formation of activated signaling complexes. Transition of the Src SH3 domain between a variety of binding partners during progression through ...

journal_title：Journal of molecular biology

authors： Wang C,Pawley NH,Nicholson LK

更新日期：2001-11-02 00:00:00

abstract：:Transcription of the ribosomal RNA operons (rrn) in Escherichia coli is subject to an antitermination mechanism whereby RNA polymerase is modified to a termination-resistant form during transit through the rrn leader region. This antitermination mechanism is unable to overcome the T1-T2 terminator cluster located at t...

journal_title：Journal of molecular biology

authors： Ghosh B,Grzadzielska E,Bhattacharya P,Peralta E,DeVito J,Das A

更新日期：1991-11-05 00:00:00

abstract：:Rigid-body docking has become quite successful in predicting the correct conformations of binary protein complexes, at least when the constituent proteins do not undergo large conformational changes upon binding. However, determining whether two given proteins interact is a more difficult problem. Successful docking p...

journal_title：Journal of molecular biology

authors： Sacquin-Mora S,Carbone A,Lavery R

更新日期：2008-10-24 00:00:00

abstract：:In Xenopus somatic cells histone H1 effects the transcriptional repression of oocyte type 5 S RNA genes, without altering the transcription of the somatic type 5 S RNA genes. Using an unambiguous nucleosome mapping method we find substantial differences between the multiple in vitro nucleosome positions on the two typ...

journal_title：Journal of molecular biology

authors： Panetta G,Buttinelli M,Flaus A,Richmond TJ,Rhodes D

更新日期：1998-09-25 00:00:00

abstract：:The adaptor protein Tks5/FISH (tyrosine kinase substrate 5/five SH3 domains, hereafter termed Tks5) is a crucial component of a protein network that controls the invasiveness of cancer cells and progression of Alzheimer's disease. Tks5 consists of an amino-terminal PX domain that is followed by five SH3 domains (SH3A-...

journal_title：Journal of molecular biology

authors： Rufer AC,Rumpf J,von Holleben M,Beer S,Rittinger K,Groemping Y

更新日期：2009-07-31 00:00:00

abstract：:The site-specific binding interaction of lac repressor with a symmetric operator sequence and of EcoRI endonuclease with its specific recognition site both exhibit a characteristic dependence of equilibrium binding constant (Kobs) on temperature, in which Kobs attains a relative maximum in the physiologically relevant...

journal_title：Journal of molecular biology

authors： Ha JH,Spolar RS,Record MT Jr

更新日期：1989-10-20 00:00:00

abstract：:The Fis protein regulates gene expression in Escherichia coli by activating or repressing transcription of a variety of genes. Fis can activate transcription when bound to DNA upstream of the RNA-polymerase-binding site, such as in the rrnB P1 promoter, or when bound to a site overlapping the -35 RNA polymerase bindin...

journal_title：Journal of molecular biology

authors： Cheng YS,Yang WZ,Johnson RC,Yuan HS

更新日期：2000-10-06 00:00:00

abstract：:The loop between the F and G beta strands (FG loop) of the bacteriophage MS2 coat protein subunit forms inter-subunit contacts around the 5-fold and 3-fold (quasi 6-fold) axes of the T=3 protein shell. In capsids, the loop is found in two very different conformations, one in B subunits, which form the 5-fold contact, ...

journal_title：Journal of molecular biology

authors： Stonehouse NJ,Valegård K,Golmohammadi R,van den Worm S,Walton C,Stockley PG,Liljas L

更新日期：1996-02-23 00:00:00

abstract：:FtsZ is part of a mid-cell cytokinetic structure termed the Z-ring that recruits a hierarchy of fission related proteins early in the bacterial cell cycle. The widely conserved ZapA has been shown to interact with FtsZ, to drive its polymerisation and to promote FtsZ filament bundling thereby contributing to the spati...

journal_title：Journal of molecular biology

authors： Low HH,Moncrieffe MC,Löwe J

更新日期：2004-08-13 00:00:00

abstract：:UDP-glucose is the universal activated form of glucose, employed in all organisms for glucosyl transfer reactions and as precursor for various activated carbohydrates. In animal and fungal metabolism, UDP-glucose is required for utilization of galactose and for the synthesis of glycogen, the major carbohydrate storage...

journal_title：Journal of molecular biology

authors： Roeben A,Plitzko JM,Körner R,Böttcher UM,Siegers K,Hayer-Hartl M,Bracher A

更新日期：2006-12-08 00:00:00

abstract：:Calmodulin (CaM) is a ubiquitous second messenger protein that regulates a variety of structurally and functionally diverse targets in response to changes in Ca(2+) concentration. CaM-dependent protein kinase II (CaMKII) and calcineurin (CaN) are the prominent CaM targets that play an opposing role in many cellular fu...

journal_title：Journal of molecular biology

authors： Yosef E,Politi R,Choi MH,Shifman JM

更新日期：2009-02-06 00:00:00

abstract：:The homotetrameric Escherichia coli single-stranded DNA binding protein (SSB) plays a central role in DNA replication, repair and recombination. E. coli SSB can bind to long single-stranded DNA (ssDNA) in multiple binding modes using all four subunits [(SSB)65 mode] or only two subunits [(SSB)35 binding mode], with th...

journal_title：Journal of molecular biology

authors： Kozlov AG,Weiland E,Mittal A,Waldman V,Antony E,Fazio N,Pappu RV,Lohman TM

更新日期：2015-02-27 00:00:00

abstract：:When influenza A virus infects host cells, its integral matrix protein M2 forms a proton-selective channel in the viral envelope. Although X-ray crystallography and NMR studies using fragment peptides have suggested that M2 stably forms a tetrameric channel irrespective of pH, the oligomeric states of the full-length ...

journal_title：Journal of molecular biology

authors： Kawano K,Yano Y,Matsuzaki K

更新日期：2014-07-15 00:00:00

abstract：:Large crystals of probable amylase/protease inhibitor-B have been grown at room temperature from ammonium sulfate solution. The crystals grow within five days to dimensions of 0.6 mm x 0.6 mm x 0.6 mm. They diffract to at least 1.7 A upon exposure to synchrotron X-rays. The crystals belong to the space group P4(1)2(1)...

journal_title：Journal of molecular biology

authors： Hwang KY,Kim KK,Min K,Eom SH,Yu YG,Kim S,Sweet RM,Suh SW

更新日期：1993-01-05 00:00:00

abstract：:The solvent-accessible surface area (As) of 46 monomeric proteins is calculated using atomic co-ordinates from high-resolution and well-refined crystal structures. The As of these proteins can be determined to within 1 to 2% and that of their individual residues to within 10 to 20%. The As values of proteins are corre...

journal_title：Journal of molecular biology

authors： Miller S,Janin J,Lesk AM,Chothia C

更新日期：1987-08-05 00:00:00

abstract：:Induction of gene expression has great potential in the treatment of many human diseases. Peptide nucleic acid (PNA) as a novel DNA-binding reagent provides an ideal system to induce gene-specific expression. In our recent studies, we have demonstrated that PNA bound to double-stranded DNA targets and, therefore, gene...

journal_title：Journal of molecular biology

authors： Wang G,Jing K,Balczon R,Xu X

更新日期：2001-11-09 00:00:00

abstract：:Twenty-four structures of pyridoxal-5'-phosphate (PLP)-dependent enzymes that represent five different folds are shown to share a common recognition pattern for the phosphate group of their PLP-ligands. All atoms that interact with the phosphate group of PLP in these proteins are organized within a two-layer structure...

journal_title：Journal of molecular biology

authors： Denesyuk AI,Denessiouk KA,Korpela T,Johnson MS

更新日期：2002-02-08 00:00:00

abstract：:alpha-Synuclein (alpha-syn) is a 140-residue protein of unknown function, involved in several neurodegenerative disorders, such as Parkinson's disease. Recently, the possible interaction between alpha-syn and polyunsaturated fatty acids has attracted a strong interest. Indeed, lipids are able to trigger the multimeriz...

journal_title：Journal of molecular biology

authors： De Franceschi G,Frare E,Bubacco L,Mammi S,Fontana A,de Laureto PP

更新日期：2009-11-20 00:00:00

abstract：:The crystal structure of the homodimer of quinone oxidoreductase from Escherichia coli has been determined using the multiple isomorphous replacement method at 2.2 A resolution and refined to an R-factor of 14.1% The crystallographic asymmetric unit contains one functional dimer with the two subunits being related by ...

journal_title：Journal of molecular biology

authors： Thorn JM,Barton JD,Dixon NE,Ollis DL,Edwards KJ

更新日期：1995-06-16 00:00:00

abstract：:A total of 76 independent spontaneous mutants in the hprt gene of V79 Chinese hamster cells have been analyzed. These mutants were obtained in two different laboratories, 17 and 59 mutants in sets 1 and 2, respectively, under different cell culture conditions. Mutation analysis was performed by amplification of hprt c...

journal_title：Journal of molecular biology

authors： Zhang LH,Vrieling H,van Zeeland AA,Jenssen D

更新日期：1992-02-05 00:00:00

abstract：:Four new crystal forms of the anti-T lectin from jackfruit (Artocarpus integrifolia) have been prepared and characterized. Three of them, two monoclinic (P21, a = 59.4 A, b = 83.3 A, c = 63.5 A, beta = 107.7 degrees; C2, a = 106.1 A, b = 53.9 A, c = 128.0 A, beta = 95.0 A) and one orthorhombic (C222(1), a = 98.1 A, b ...

journal_title：Journal of molecular biology

authors： Banerjee R,Dhanaraj V,Mahanta SK,Surolia A,Vijayan M

更新日期：1991-10-05 00:00:00