当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Crystal structures of MS2 capsids with mutations in the subunit FG loop.

Crystal structures of MS2 capsids with mutations in the subunit FG loop.

Abstract：

:The loop between the F and G beta strands (FG loop) of the bacteriophage MS2 coat protein subunit forms inter-subunit contacts around the 5-fold and 3-fold (quasi 6-fold) axes of the T=3 protein shell. In capsids, the loop is found in two very different conformations, one in B subunits, which form the 5-fold contact, and one in A and C subunits, which form the quasi 6-fold contact. One proline residue, Pro78, is strictly conserved in the coat protein of all related bacteriophages, and in the case of MS2 this proline residue is preceded by a cis peptide bond in the B subunit. In order to probe the role of the FG loop in capsid assembly, we have determined the crystal structures of two MS2 capsids, formed by coat proteins with mutations at two positions in the FG loop, P78N or E76D. These mutants show conformational changes in the FG loops that explain the reduced temperature stability of the capsids. The P78N mutant has a normal trans peptide bond at position 78.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Stonehouse NJ,Valegård K,Golmohammadi R,van den Worm S,Walton C,Stockley PG,Liljas L

doi

10.1006/jmbi.1996.0089

subject

Has Abstract

pub_date

1996-02-23 00:00:00

pages

330-9

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(96)90089-3

journal_volume

256

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • X-ray analyses of aspartic proteinases. V. Structure and refinement at 2.0 A resolution of the aspartic proteinase from Mucor pusillus.

    abstract：:The structure of mucor pusillus pepsin (EC 3.4.23.6), the aspartic proteinase from Mucor pusillus, has been refined to a crystallographic R-factor of 16.2% at 2.0 A resolution. The positions of 2638 protein atoms, 221 solvent atoms and a sulphate ion have been determined with an estimated root-mean-square (r.m.s.) err...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：

    authors： Newman M,Watson F,Roychowdhury P,Jones H,Badasso M,Cleasby A,Wood SP,Tickle IJ,Blundell TL

    更新日期：1993-03-05 00:00:00

  • The catalytic subunit of human protein kinase CK2 structurally deviates from its maize homologue in complex with the nucleotide competitive inhibitor emodin.

    abstract：:The Ser/Thr kinase CK2 (former name: casein kinase 2) is a heterotetrameric enzyme composed of two catalytic chains (CK2alpha) attached to a dimer of noncatalytic subunits. Together with the cyclin-dependent kinases and the mitogen-activated protein kinases, CK2alpha belongs to the CMGC family of the eukaryotic protei...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.01.008

    authors： Raaf J,Klopffleisch K,Issinger OG,Niefind K

    更新日期：2008-03-14 00:00:00

  • Autoinhibition of ETV6 (TEL) DNA binding: appended helices sterically block the ETS domain.

    abstract：:ETV6 (or TEL), a transcriptional repressor belonging to the ETS family, is frequently involved in chromosomal translocations linked with human cancers. It displays a DNA-binding mode distinct from other ETS proteins due to the presence of a self-associating PNT domain. In this study, we used NMR spectroscopy to dissec...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.05.010

    authors： Coyne HJ 3rd,De S,Okon M,Green SM,Bhachech N,Graves BJ,McIntosh LP

    更新日期：2012-08-03 00:00:00

  • Crystallization of recA protein from Proteus mirabilis.

    abstract：:The recA protein from Proteus mirabilis, which is homologous to the Escherichia coli protein, forms crystals in the orthorhombic space group P2(1)2(1)2(1). There are two 38,000 molecular weight subunits in the asymmetric unit and the unit cell dimensions are a = 57.5, b = 127.0 and c = 157.0 A. ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(86)90486-9

    authors： Weber IT,Steitz TA

    更新日期：1986-03-05 00:00:00

  • Crystal structure of human ribosomal protein L10 core domain reveals eukaryote-specific motifs in addition to the conserved fold.

    abstract：:A phylogenetically conserved ribosomal protein L16p/L10e organizes the architecture of the aminoacyl tRNA binding site on the large ribosomal subunit. Eukaryotic L10 also exhibits a variety of cellular activities, and, in particular, human L10 is known as a putative tumor suppressor, QM. We have determined the 2.5-A c...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.01.003

    authors： Nishimura M,Kaminishi T,Takemoto C,Kawazoe M,Yoshida T,Tanaka A,Sugano S,Shirouzu M,Ohkubo T,Yokoyama S,Kobayashi Y

    更新日期：2008-03-21 00:00:00

  • Core side-chain packing and backbone conformation in Lpp-56 coiled-coil mutants.

    abstract：:Native proteins exhibit precise geometric packing of atoms in their hydrophobic interiors. Nonetheless, controversy remains about the role of core side-chain packing in specifying and stabilizing the folded structures of proteins. Here we investigate the role of core packing in determining the conformation and stabili...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(02)00138-9

    authors： Liu J,Cao W,Lu M

    更新日期：2002-05-03 00:00:00

  • Predictive QM/MM Modeling of Modulations in Protein-Protein Binding by Lysine Methylation.

    abstract：:Lysine methylation is a key regulator of protein-protein binding. The amine group of lysine can accept up to three methyl groups, and experiments show that protein-protein binding free energies are sensitive to the extent of methylation. These sensitivities have been rationalized in terms of chemical and structural fe...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.166745

    authors： Rahman S,Wineman-Fisher V,Al-Hamdani Y,Tkatchenko A,Varma S

    更新日期：2021-02-05 00:00:00

  • New efficient statistical sequence-dependent structure prediction of short to medium-sized protein loops based on an exhaustive loop classification.

    abstract：:A bank of 13,563 loops from three to eight amino acid residues long, representing motifs between two consecutive regular secondary structures, has been derived from protein structures presenting less than 95 % sequence identity. Statistical analyses of occurrences of conformations and residues revealed length-dependen...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2826

    authors： Wojcik J,Mornon JP,Chomilier J

    更新日期：1999-06-25 00:00:00

  • Deletion analysis of the che operon in the archaeon Halobacterium salinarium.

    abstract：:Halobacterium salinarium is a chemo- and phototactic archaeon whose signal transduction pathway includes the classical two-component system made up of CheA and CheY. Deletion analysis of the che operon in H. salinarium has been undertaken. Following the removal of the entire operon, the importance of each of the four ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0267

    authors： Rudolph J,Oesterhelt D

    更新日期：1996-05-17 00:00:00

  • Transcription of repA, the gene of the initiation protein of the Pseudomonas plasmid pPS10, is autoregulated by interactions of the RepA protein at a symmetrical operator.

    abstract：:Transcription of the repA gene of the Pseudomonas plasmid pPS10 is initiated from a sigma 70 type promoter located 81 bp upstream from the repA gene, extends through the repA gene and the adjacent open reading frame, and ends 1114 nucleotides downstream. The repA promoter is repressed by interactions of the RepA prote...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.0134

    authors： García de Viedma D,Giraldo R,Ruiz-Echevarría MJ,Lurz R,Díaz-Orejas R

    更新日期：1995-03-24 00:00:00

  • Structural alteration in alternating adenine-thymine sequences in positively supercoiled DNA.

    abstract：:An alternating adenine-thymine tract in a relaxed closed circular plasmid was found to become strongly reactive to osmium tetroxide in the presence of actinomycin D. We suggest that this is due to a local overwinding of the alternating tract as a result of positive supercoiling induced by intercalation of the antibiot...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90555-k

    authors： McClellan JA,Lilley DM

    更新日期：1991-05-20 00:00:00

  • A bisubstrate analog induces unexpected conformational changes in phosphoglycerate kinase from Trypanosoma brucei.

    abstract：:The glycolytic enzyme phosphoglycerate kinase (PGK) catalyzes phosphoryl transfer between 1,3-bis-phosphoglycerate and ADP to form 3-phosphoglycerate and ATP. During catalysis, a major hinge bending motion occurs which brings the N and C-terminal enzyme domains and their bound substrates together and in-line for phosp...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.1835

    authors： Bernstein BE,Williams DM,Bressi JC,Kuhn P,Gelb MH,Blackburn GM,Hol WG

    更新日期：1998-06-26 00:00:00

  • Crystallization and preliminary X-ray data of a phleomycin-binding protein from Streptoalloteichus hindustanus.

    abstract：:Large single crystals of a glycopeptide resistance protein encoded by the SH-ble gene from Streptoalloteichus hindustanus have been grown using vapor diffusion techniques with ammonium sulfate as the precipitant. The diffraction pattern extends to 2.0 A resolution. The crystals belong to space group P4(1)2(1)2 or P4(3...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(89)90476-2

    authors： Rondeau JM,Cagnon C,Moras D,Masson JM

    更新日期：1989-06-05 00:00:00

  • Repression and activation of promoter-bound RNA polymerase activity by Gal repressor.

    abstract：:By binding to the DNA site OE at position -60.5 in the gal operon, the GalR protein activates transcription from the P2 promoter located on the opposite face of DNA (position -5) and represses transcription from the P1 promoter located on the same face (position +1). GalR increases RNA polymerase binding at P2 and inh...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1221

    authors： Choy HE,Hanger RR,Aki T,Mahoney M,Murakami K,Ishihama A,Adhya S

    更新日期：1997-09-26 00:00:00

  • NMR solution structure of Mob1, a mitotic exit network protein and its interaction with an NDR kinase peptide.

    abstract：:Proteins of the Mob1/phocein family are found in all eukaryotic cells. In yeast, they are activating subunits of Dbf2-related protein kinases involved in cell cycle control. Despite the wide occurrence of these proteins, their biological functions remain poorly understood. Here we report the solution structure of the ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.01.010

    authors： Ponchon L,Dumas C,Kajava AV,Fesquet D,Padilla A

    更新日期：2004-03-12 00:00:00

  • Evolution of specific protein-protein interaction sites following gene duplication.

    abstract：:Gene duplication is a common evolutionary process that leads to the expansion and functional diversification of protein subfamilies. The evolutionary events that cause paralogous proteins to bind different protein ligands (functionally diverged interfaces) are investigated and compared to paralogous proteins that bind...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.06.039

    authors： Aiello D,Caffrey DR

    更新日期：2012-10-19 00:00:00

  • Correlations between scaffold/matrix attachment region (S/MAR) binding activity and DNA duplex destabilization energy.

    abstract：:Scaffold or matrix-attachment regions (S/MARs) are thought to be involved in the organization of eukaryotic chromosomes and in the regulation of several DNA functions. Their characteristics are conserved between plants and humans, and a variety of biological activities have been associated with them. The identificatio...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.11.073

    authors： Bode J,Winkelmann S,Götze S,Spiker S,Tsutsui K,Bi C,A K P,Benham C

    更新日期：2006-04-28 00:00:00

  • Identification of protein interaction partners and protein-protein interaction sites.

    abstract：:Rigid-body docking has become quite successful in predicting the correct conformations of binary protein complexes, at least when the constituent proteins do not undergo large conformational changes upon binding. However, determining whether two given proteins interact is a more difficult problem. Successful docking p...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.08.002

    authors： Sacquin-Mora S,Carbone A,Lavery R

    更新日期：2008-10-24 00:00:00

  • Oligomerization of the SPP1 scaffolding protein.

    abstract：:Viral scaffolding proteins direct polymerization of major capsid protein subunits into icosahedral procapsid structures. The scaffolding protein of bacteriophage SPP1 was engineered with a C-terminal hexahistidine tag (gp11-His(6)) and purified. The protein is an alpha-helical-rich molecule with a very elongated shape...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.02.028

    authors： Poh SL,el Khadali F,Berrier C,Lurz R,Melki R,Tavares P

    更新日期：2008-05-02 00:00:00

  • A structural-informatics approach for mining beta-sheets: locating sheets in intermediate-resolution density maps.

    abstract：:Here, we report a new computational method, called sheetminer, for mining beta-sheets in the density maps at intermediate resolutions of 6 to 10A. The method employs a multi-step ad hoc morphological analysis of density maps to identify the unique characteristics of beta-sheets. It was tested on density maps from 12 p...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00859-3

    authors： Kong Y,Ma J

    更新日期：2003-09-12 00:00:00

  • Distinct functions of N and C-terminal domains of GreA, an Escherichia coli transcript cleavage factor.

    abstract：:The prokaryotic transcription factors GreA and GreB are involved in the regulation of transcript elongation by RNA polymerase (RNAP). Their known activities include suppression of transcription arrest, enhancement of transcription fidelity, and facilitation of the transition from abortive initiation to productive elon...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1545

    authors： Koulich D,Nikiforov V,Borukhov S

    更新日期：1998-02-20 00:00:00

  • Prohead and DNA-gp3-dependent ATPase activity of the DNA packaging protein gp16 of bacteriophage phi 29.

    abstract：:The ATPase activity of the DNA packaging protein gp16 (gene product 16) of bacteriophage phi 29 was studied in the completely defined in-vitro assembly system. ATP was hydrolyzed to ADP and Pi in the packaging reaction that included purified proheads, DNA-gp3 and gp16. Approximately one molecule of ATP was used in the...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90121-5

    authors： Guo P,Peterson C,Anderson D

    更新日期：1987-09-20 00:00:00

  • Early events in murine erythroleukemia cells induced to differentiate. Accumulation and gene expression of the transformation-associated cellular protein p53.

    abstract：:Oncogenes may play a crucial role in the genetic program of cellular differentiation; even, probably, at a very early stage in this program, which can be described as pre-commitment. We have investigated the variation in, and the control level of, the accumulation of the transformation-associated cellular protein p53 ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(88)90333-6

    authors： Khochbin S,Principaud E,Chabanas A,Lawrence JJ

    更新日期：1988-03-05 00:00:00

  • Crystal structure of the C-terminal domain of S.cerevisiae eIF5.

    abstract：:eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a critical role in pre-initiation complex assembly and correct AUG selection during eukaryotic translation initiation. eIF5 is involved in the formation of the multifactor complex (MFC), an important intermediate of the 43S pre-initiation complex. The C-...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.03.037

    authors： Wei Z,Xue Y,Xu H,Gong W

    更新日期：2006-05-26 00:00:00

  • Visualization of the unwinding of long DNA chains by the herpes simplex virus type 1 UL9 protein and ICP8.

    abstract：:UL9 protein and ICP8 encoded by the herpes simplex virus type 1 (HSV-1) were shown to catalyze a highly active, non-origin-dependent unwinding of DNA. UL9 protein, the HSV-1 origin binding protein, as a modest helicase activity that is greatly stimulated by the HSV-1 single strand (ss) binding protein, ICP8. Here, ele...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0287

    authors： Makhov AM,Boehmer PE,Lehman IR,Griffith JD

    更新日期：1996-05-24 00:00:00

  • Monomers of the Escherichia coli SSB-1 mutant protein bind single-stranded DNA.

    abstract：:The Escherichia coli wild-type single strand binding (SSB) protein is a stable tetramer that binds to single-stranded (ss) DNA in its role in DNA replication, recombination and repair. The ssb-1 mutation, a substitution of tyrosine for histidine-55 within the SSB-1 protein, destabilizes the tetramer with respect to mo...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90611-9

    authors： Bujalowski W,Lohman TM

    更新日期：1991-01-05 00:00:00

  • Assembly of robust bacterial microcompartment shells using building blocks from an organelle of unknown function.

    abstract：:Bacterial microcompartments (BMCs) sequester enzymes from the cytoplasmic environment by encapsulation inside a selectively permeable protein shell. Bioinformatic analyses indicate that many bacteria encode BMC clusters of unknown function and with diverse combinations of shell proteins. The genome of the halophilic m...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2014.02.025

    authors： Lassila JK,Bernstein SL,Kinney JN,Axen SD,Kerfeld CA

    更新日期：2014-05-29 00:00:00

  • Conserved C-terminal region of global repressor KorA of broad-host-range plasmid RK2 is required for co-operativity between KorA and a second RK2 global regulator, KorB.

    abstract：:KorA and KorB proteins of IncP1 plasmid RK2 are encoded in the central control region (ccr) of the plasmid and act as global regulators of plasmid genes for replication, transfer and stable inheritance. KorA represses seven promoters on RK2, by binding to a defined operator site, OA, which always occurs in promoter re...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2761

    authors： Kostelidou K,Jones AC,Thomas CM

    更新日期：1999-06-04 00:00:00

  • Genetic dissection of the consensus sequence for the class 2 and class 3 flagellar promoters.

    abstract：:Computational searches for DNA binding sites often utilize consensus sequences. These search models make assumptions that the frequency of a base pair in an alignment relates to the base pair's importance in binding and presume that base pairs contribute independently to the overall interaction with the DNA-binding pr...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.04.043

    authors： Wozniak CE,Hughes KT

    更新日期：2008-06-20 00:00:00

  • X-ray diffraction analysis of crystals of bovine platelet factor 4.

    abstract：:Bovine platelet factor 4 has been crystallized by "vapor dilution" in space group P2(1)2(1)2(1), a = 63.7 A, b = 66.7 A, c = 80.5 A, with four molecules, each 9505 Mr, in the asymmetric unit. The crystals diffract X-rays to better than 2.8 A resolution. ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(84)90497-2

    authors： St Charles R,Ciaglowski RE,Walz D,Edwards BF

    更新日期：1984-07-05 00:00:00