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Crystallization and preliminary X-ray crystallographic analysis of probable amylase/protease inhibitor-B from rice seeds.

Abstract：

:Large crystals of probable amylase/protease inhibitor-B have been grown at room temperature from ammonium sulfate solution. The crystals grow within five days to dimensions of 0.6 mm x 0.6 mm x 0.6 mm. They diffract to at least 1.7 A upon exposure to synchrotron X-rays. The crystals belong to the space group P4(1)2(1)2 (or P4(3)2(1)2) with unit cell dimensions of a = 38.02 A and c = 98.98 A. The presence of one molecule per asymmetric unit gives the unit cell volume per protein mass (Vm) of 1.99 A3/Da and the solvent fraction of 38.2% by volume. X-ray data have been collected to 2.0 A Bragg spacing from native crystals.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Hwang KY,Kim KK,Min K,Eom SH,Yu YG,Kim S,Sweet RM,Suh SW

doi

10.1006/jmbi.1993.1027

subject

Has Abstract

pub_date

1993-01-05 00:00:00

pages

255-7

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(83)71027-2

journal_volume

229

pub_type

杂志文章

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