当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > The EGF-like protein dlk1 inhibits notch signaling and potentiates adipogenesis of mesenchymal cells.

The EGF-like protein dlk1 inhibits notch signaling and potentiates adipogenesis of mesenchymal cells.

Abstract：

:The EGF-like homeotic gene Dlk1 appears to function as an inhibitor of adipogenesis. Overexpression of Dlk1 prevents adipogenesis of 3T3-L1 cells. Dlk1-deficient mice are obese; however, adipose tissue still develops in Fc-dlk1 transgenic mice, suggesting that Dlk1 is not a strict inhibitor of adipogenesis. To clarify the role of Dlk1 in adipogenesis, we studied whether Dlk1 could act differently on this process depending upon the differentiation state of the precursor cells. We found that Dlk1 is a potentiator of adipogenesis for mesenchymal C3H10T1/2 cells. This potentiating effect can be triggered by overexpressing the entire protein or the extracellular EGF-like-containing region, but not by overexpressing the intracellular dlk1 sequence. In addition, coculture of C3H10T1/2 cells with other cells expressing Dlk1, but not with cells lacking Dlk1 expression, enhances their adipogenic response. Potentiation of adipogenesis by Dlk1 was associated with changes in the activation of ERK1/2 after IGFI/insulin induction. Finally, as reported with other cells, dlk1 functioned as a Notch signaling inhibitor in C3H10T1/2 cells, but inhibition of Notch1 expression prevented the potentiating effects of Dlk1 in adipogenesis. These data suggest that Dlk1 may potentiate or inhibit adipogenesis depending upon the cellular context, and that Notch1 expression and activation are important factors in this context.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Nueda ML,Baladrón V,Sánchez-Solana B,Ballesteros MA,Laborda J

doi

10.1016/j.jmb.2006.10.043

subject

Has Abstract

pub_date

2007-04-13 00:00:00

pages

1281-93

issue

5

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(06)01415-X

journal_volume

367

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Co-axial association of recombinant eye lens aquaporin-0 observed in loosely packed 3D crystals.

    abstract：:Aquaporin-0 (AQP0) is the major membrane protein in vertebrate eye lenses. It has been proposed that AQP0 tetramers mediate contact between membranes of adjacent lens fiber cells, which would be consistent with the extraordinarily narrow inter-cellular spacing. We have obtained 3D crystals of recombinant bovine AQP0 t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.10.032

    authors： Palanivelu DV,Kozono DE,Engel A,Suda K,Lustig A,Agre P,Schirmer T

    更新日期：2006-01-27 00:00:00

  • Nucleotide sequences of immunoglobulin-epsilon pseudogenes in man and apes and their phylogenetic relationships.

    abstract：:To understand the phylogenetic relationships between hominoids, the nucleotide sequences of immunoglobulin-epsilon processed pseudogenes from chimpanzee, gorilla and orangutan were determined. The basic structures of these processed pseudogenes agreed with their human counterpart. Although the degrees of nucleotide di...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(89)90366-5

    authors： Ueda S,Watanabe Y,Saitou N,Omoto K,Hayashida H,Miyata T,Hisajima H,Honjo T

    更新日期：1989-01-05 00:00:00

  • Resolution of Holliday junction substrates by human topoisomerase I.

    abstract：:Prompted by the close relationship between tyrosine recombinases and type IB topoisomerases we have investigated the ability of human topoisomerase I to resolve the typical intermediate of recombinase catalysis, the Holliday junction. We demonstrate that human topoisomerase I catalyzes unidirectional resolution of a s...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.10.050

    authors： Hede MS,Petersen RL,Frøhlich RF,Krüger D,Andersen FF,Andersen AH,Knudsen BR

    更新日期：2007-01-26 00:00:00

  • Toward Decoding Bioelectric Events in Xenopus Embryogenesis: New Methodology for Tracking Interplay Between Calcium and Resting Potentials In Vivo.

    abstract：:Although chemical signaling during embryogenesis is readily addressed by a plethora of available techniques, the developmental functions of ionic signaling are still poorly understood. It is increasingly realized that bioelectric events in nonneural cells are critical for pattern regulation, but their study has been h...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2019.10.029

    authors： McMillen P,Novak R,Levin M

    更新日期：2020-01-17 00:00:00

  • Dimeric crystal structure of rabbit L-gulonate 3-dehydrogenase/lambda-crystallin: insights into the catalytic mechanism.

    abstract：:l-Gulonate 3-dehydrogenase (GDH) is a bifunctional dimeric protein that functions not only as an NAD(+)-dependent enzyme in the uronate cycle but also as a taxon-specific lambda-crystallin in rabbit lens. Here we report the first crystal structure of GDH in both apo form and NADH-bound holo form. The GDH protomer cons...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.06.069

    authors： Asada Y,Kuroishi C,Ukita Y,Sumii R,Endo S,Matsunaga T,Hara A,Kunishima N

    更新日期：2010-09-03 00:00:00

  • Crystallographic structure of phosphofructokinase-2 from Escherichia coli in complex with two ATP molecules. Implications for substrate inhibition.

    abstract：:Phosphofructokinase-1 and -2 (Pfk-1 and Pfk-2, respectively) from Escherichia coli belong to different homologous superfamilies. However, in spite of the lack of a common ancestor, they share the ability to catalyze the same reaction and are inhibited by the substrate MgATP. Pfk-2, an ATP-dependent 6-phosphofructokina...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.08.029

    authors： Cabrera R,Ambrosio AL,Garratt RC,Guixé V,Babul J

    更新日期：2008-11-14 00:00:00

  • On the nature of conformational openings: native and unfolded-state hydrogen and thiol-disulfide exchange studies of ferric aquomyoglobin.

    abstract：:Native-state amide hydrogen exchange (HX) of proteins in the presence of denaturant has provided valuable details on the structures of equilibrium folding intermediates. Here, we extend HX theory to model thiol group exchange (SX) in single cysteine-containing variants of sperm whale ferric aquomyoglobin. SX is comple...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.5117

    authors： Feng Z,Butler MC,Alam SL,Loh SN

    更新日期：2001-11-16 00:00:00

  • Characterization of murine erythropoietin receptor genes.

    abstract：:We have isolated and characterized the murine genomic and complementary DNAs encoding erythropoietin (Epo) receptor from Epo-responsive and unresponsive mouse erythroleukemia cells. Two classes of Epo receptor cDNAs were isolated from Epo-responsive cells. One is a 55,000 Mr membrane-bound Epo receptor, and the other ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(90)90384-x

    authors： Kuramochi S,Ikawa Y,Todokoro K

    更新日期：1990-12-05 00:00:00

  • Protein titration in the crystal state.

    abstract：:Proteins are complex structures whose overall stability critically depends on a delicate balance of numerous interactions of similar strength, which are markedly influenced by their environment. Here, we present an analysis of the effect of pH on a protein structure in the crystalline state using RNase A as a model sy...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3093

    authors： Berisio R,Lamzin VS,Sica F,Wilson KS,Zagari A,Mazzarella L

    更新日期：1999-10-01 00:00:00

  • The 2.0A resolution structure of the catalytic portion of a cyanobacterial membrane-bound manganese superoxide dismutase.

    abstract：:Cyanobacteria are shown to be unique in containing membrane-bound manganese superoxide dismutases (MnSOD). They are homodimeric type 2 membrane proteins that protect this phototrophic organism against oxidative stress. We have determined, for the first time, the 2.0A resolution structure of the catalytic portion of th...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00624-1

    authors： Atzenhofer W,Regelsberger G,Jacob U,Peschek G,Furtmüller P,Huber R,Obinger C

    更新日期：2002-08-16 00:00:00

  • Molecular and functional characterization of a carbon starvation gene of Escherichia coli.

    abstract：:Escherichia coli induces the synthesis of at least 30 proteins at the onset of carbon starvation, two-thirds of which are positively regulated by the cyclic AMP (cAMP) and cAMP receptor protein (CRP) complex. Two of the cAMP-CRP-dependent genes mapped to 14 and 93 minutes of the chromosome and are designated cstA and ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90879-b

    authors： Schultz JE,Matin A

    更新日期：1991-03-05 00:00:00

  • Structural studies of HIV-1 Tat protein.

    abstract：:Tat (trans-activator) proteins are early RNA binding proteins regulating lentiviral transcription. These proteins are necessary components in the life cycle of all known lentiviruses, such as the human immunodeficiency viruses (HIV) or the equine infectious anemia virus (EIAV). Tat proteins are thus ideal targets for ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0158

    authors： Bayer P,Kraft M,Ejchart A,Westendorp M,Frank R,Rösch P

    更新日期：1995-04-07 00:00:00

  • The Molecular Basis of MeCP2 Function in the Brain.

    abstract：:MeCP2 is a reader of the DNA methylome that occupies a large proportion of the genome due to its high abundance and the frequency of its target sites. It has been the subject of extensive study because of its link with 'MECP2-related disorders', of which Rett syndrome is the most prevalent. This review integrates evid...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2019.10.004

    authors： Tillotson R,Bird A

    更新日期：2019-10-17 00:00:00

  • Weighting aligned protein or nucleic acid sequences to correct for unequal representation.

    abstract：:Aligned sequences from the same family (e.g. the haemoglobins) are seldom representative of the entire family. This is because (1) the sequence databases are heavily skewed toward a small number of organisms and (2) only a minute fraction of all the different family members have been sequenced. For many applications, ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(99)80003-5

    authors： Sibbald PR,Argos P

    更新日期：1990-12-20 00:00:00

  • Recapitulation of protein family divergence using flexible backbone protein design.

    abstract：:We use flexible backbone protein design to explore the sequence and structure neighborhoods of naturally occurring proteins. The method samples sequence and structure space in the vicinity of a known sequence and structure by alternately optimizing the sequence for a fixed protein backbone using rotamer based sequence...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.11.062

    authors： Saunders CT,Baker D

    更新日期：2005-02-18 00:00:00

  • The entropy cost of protein association.

    abstract：:The temperature induced unfolding/dissociation of the dimeric subtilisin inhibitor from Streptomyces and its mutant D83C having an S-S crosslink between the subunits has been studied calorimetrically. Comparison of the entropies measured at different concentrations of dimer showed that the entropy cost of crosslinking...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1368

    authors： Tamura A,Privalov PL

    更新日期：1997-11-14 00:00:00

  • Structure and substrate binding properties of cobB, a Sir2 homolog protein deacetylase from Escherichia coli.

    abstract：:Sirtuins are NAD+-dependent protein deacetylase enzymes that are broadly conserved from bacteria to human, and have been implicated to play important roles in gene regulation, metabolism and longevity. cobB is a bacterial sirtuin that deacetylates acetyl-CoA synthetase (Acs) at an active site lysine to stimulate its e...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.01.060

    authors： Zhao K,Chai X,Marmorstein R

    更新日期：2004-03-26 00:00:00

  • Packaging of a unit-length viral genome: the role of nucleotides and the gpD decoration protein in stable nucleocapsid assembly in bacteriophage lambda.

    abstract：:The developmental pathways for a variety of eukaryotic and prokaryotic double-stranded DNA viruses include packaging of viral DNA into a preformed procapsid structure, catalyzed by terminase enzymes and fueled by ATP hydrolysis. In most instances, a capsid expansion process accompanies DNA packaging, which significant...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.08.063

    authors： Yang Q,Maluf NK,Catalano CE

    更新日期：2008-11-28 00:00:00

  • Use of a 3D structure data base for understanding sequence-dependent conformational aspects of DNA.

    abstract：:The roll-twist-slide correlation in the DNA crystal structures that are collected in the Nucleic Acid Data Base is analyzed in order to obtain a general understanding of the effects of the nucleotide sequence on the 3D structure of a dinucleotide step. It is concluded that the differences between the pyrimidine bases ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1406

    authors： Suzuki M,Amano N,Kakinuma J,Tateno M

    更新日期：1997-12-05 00:00:00

  • Folding mechanism of indole-3-glycerol phosphate synthase from Sulfolobus solfataricus: a test of the conservation of folding mechanisms hypothesis in (beta(alpha))(8) barrels.

    abstract：:As a test of the hypothesis that folding mechanisms are better conserved than sequences in TIM barrels, the equilibrium and kinetic folding mechanisms of indole-3-glycerol phosphate synthase (sIGPS) from the thermoacidophilic archaebacterium Sulfolobus solfataricus were compared to the well-characterized models of the...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00557-0

    authors： Forsyth WR,Matthews CR

    更新日期：2002-07-26 00:00:00

  • Proteolytic and conformational control of virus capsid maturation: the bacteriophage HK97 system.

    abstract：:Bacteriophage capsid assembly pathways provide excellent model systems to study large-scale conformational changes and other mechanisms that regulate the formation of macromolecular complexes. These capsids are formed from proheads: relatively fragile precursor particles which mature by undergoing extensive remodeling...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0538

    authors： Conway JF,Duda RL,Cheng N,Hendrix RW,Steven AC

    更新日期：1995-10-13 00:00:00

  • Folding of amphipathic alpha-helices on membranes: energetics of helix formation by melittin.

    abstract：:Membranes have a potent ability to promote secondary structure formation in a wide range of membrane-active peptides, believed to be due to a reduction through hydrogen bonding of the energetic cost of partitioning peptide bonds. This process is of fundamental importance for understanding the mechanism of action of to...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2346

    authors： Ladokhin AS,White SH

    更新日期：1999-01-29 00:00:00

  • Structural plasticity of the nuclear pore complex.

    abstract：:The nuclear pore complex (NPC) is strategically located at continuous junctions of the inner and outer nuclear membranes to catalyze macromolecular transport, without impending the diffusion of small molecules. In this paper, the structural plasticity of 4412 NPCs in isolated nuclear envelopes has been evaluated, util...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(95)80050-6

    authors： Akey CW

    更新日期：1995-04-28 00:00:00

  • Interaction of monomeric and dimeric kinesin with microtubules.

    abstract：:The binding stoichiometry of kinesin to microtubules was determined using several biochemical and biophysical approaches (chemical crosslinking, binding assays, scanning transmission electron microscopy (STEM), image reconstruction, and X-ray scattering). The results show that each tubulin dimer associates with one ki...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1503

    authors： Thormählen M,Marx A,Müller SA,Song Y,Mandelkow EM,Aebi U,Mandelkow E

    更新日期：1998-02-06 00:00:00

  • Structural basis for the dual recognition of IL-12 and IL-23 by ustekinumab.

    abstract：:Interleukin (IL)-12 and IL-23 are heterodimeric proinflammatory cytokines that share a common p40 subunit, paired with p35 and p19 subunits, respectively. They represent an attractive class of therapeutic targets for the treatment of psoriasis and other immune-mediated diseases. Ustekinumab is a fully human monoclonal...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.07.046

    authors： Luo J,Wu SJ,Lacy ER,Orlovsky Y,Baker A,Teplyakov A,Obmolova G,Heavner GA,Richter HT,Benson J

    更新日期：2010-10-08 00:00:00

  • Ion-coupling determinants of Na+-driven and H+-driven flagellar motors.

    abstract：:The bacterial flagellar motor is a tiny molecular machine that uses a transmembrane flux of H(+) or Na(+) ions to drive flagellar rotation. In proton-driven motors, the membrane proteins MotA and MotB interact via their transmembrane regions to form a proton channel. The sodium-driven motors that power the polar flage...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00096-2

    authors： Asai Y,Yakushi T,Kawagishi I,Homma M

    更新日期：2003-03-21 00:00:00

  • Divergent genetic control of protein solubility and conformational quality in Escherichia coli.

    abstract：:In bacteria, protein overproduction results in the formation of inclusion bodies, sized protein aggregates showing amyloid-like properties such as seeding-driven formation, amyloid-tropic dye binding, intermolecular beta-sheet architecture and cytotoxicity on mammalian cells. During protein deposition, exposed hydroph...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.09.004

    authors： García-Fruitós E,Martínez-Alonso M,Gonzàlez-Montalbán N,Valli M,Mattanovich D,Villaverde A

    更新日期：2007-11-16 00:00:00

  • Analysis of the tobacco chloroplast DNA replication origin (oriB) downstream of the 23 S rRNA gene.

    abstract：:We have mapped the origin of DNA replication (oriB) downstream of the 23 S rRNA gene in each copy of the inverted repeat (IR) of tobacco chloroplast DNA between positions 130,502 and 131,924 (IR(A)) by a combination of approaches. In vivo chloroplast DNA replication intermediates were examined by two-dimensional agaro...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.0972

    authors： Kunnimalaiyaan M,Shi F,Nielsen BL

    更新日期：1997-05-02 00:00:00

  • Functional and structural role of amino acid residues in the odd-numbered transmembrane alpha-helices of the bovine mitochondrial oxoglutarate carrier.

    abstract：:The mitochondrial oxoglutarate carrier (OGC) plays an important role in the malate-aspartate shuttle, the oxoglutarate-isocitrate shuttle and gluconeogenesis. To establish amino acid residues that are important for function, each residue in the transmembrane alpha-helices H1, H3 and H5 was replaced systematically by a...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.03.048

    authors： Cappello AR,Miniero DV,Curcio R,Ludovico A,Daddabbo L,Stipani I,Robinson AJ,Kunji ER,Palmieri F

    更新日期：2007-06-01 00:00:00

  • Distinct functions of N and C-terminal domains of GreA, an Escherichia coli transcript cleavage factor.

    abstract：:The prokaryotic transcription factors GreA and GreB are involved in the regulation of transcript elongation by RNA polymerase (RNAP). Their known activities include suppression of transcription arrest, enhancement of transcription fidelity, and facilitation of the transition from abortive initiation to productive elon...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1545

    authors： Koulich D,Nikiforov V,Borukhov S

    更新日期：1998-02-20 00:00:00