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The entropy cost of protein association.

Abstract：

:The temperature induced unfolding/dissociation of the dimeric subtilisin inhibitor from Streptomyces and its mutant D83C having an S-S crosslink between the subunits has been studied calorimetrically. Comparison of the entropies measured at different concentrations of dimer showed that the entropy cost of crosslinking is small. Its value at the standard concentration of 1 M is of the order of -(5+/-4) cal/K.mol, i.e. it is more than one order of magnitude smaller than the values of translational entropies calculated on the base of statistical thermodynamics, using in particular the Sackur-Tetrode equation, and is close to the cratic entropy value suggested by classical mixing theory.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Tamura A,Privalov PL

doi

10.1006/jmbi.1997.1368

subject

Has Abstract

pub_date

1997-11-14 00:00:00

pages

1048-60

issue

5

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(97)91368-1

journal_volume

273

pub_type

杂志文章

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