Abstract：
:The hairpin ribozyme belongs to the family of small catalytic RNAs that cleave RNA substrates in a reversible reaction that generates 2',3'-cyclic phosphate and 5'-hydroxyl termini. The hairpin catalytic motif was discovered in the negative strand of the tobacco ringspot virus satellite RNA, where hairpin ribozyme-mediated self-cleavage and ligation reactions participate in processing RNA replication intermediates. The self-cleaving hairpin, hammerhead, hepatitis delta and Neurospora VS RNAs each adopt unique structures and exploit distinct kinetic and catalytic mechanisms despite catalyzing the same chemical reactions. Mechanistic studies of hairpin ribozyme reactions provided early evidence that, like protein enzymes, RNA enzymes are able to exploit a variety of catalytic strategies. In contrast to the hammerhead and Tetrahymena ribozyme reactions, hairpin-mediated cleavage and ligation proceed through a catalytic mechanism that does not require direct coordination of metal cations to phosphate or water oxygens. The hairpin ribozyme is a better ligase than it is a nuclease while the hammerhead reaction favors cleavage over ligation of bound products by nearly 200-fold. Recent structure-function studies have begun to yield insights into the molecular bases of these unique features of the hairpin ribozyme.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Fedor MJdoi
10.1006/jmbi.2000.3560keywords:
subject
Has Abstractpub_date
2000-03-24 00:00:00pages
269-91issue
2eissn
0022-2836issn
1089-8638pii
S0022-2836(00)93560-5journal_volume
297pub_type
杂志文章,评审abstract：:Ubiquitination is one of the most prevalent posttranslational modifications in eukaryotic cells, with functional importance in protein degradation, subcellular localization and signal transduction pathways. Immunoaffinity enrichment coupled with quantitative mass spectrometry enables the in-depth characterization of p...
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更新日期：1995-07-21 00:00:00
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更新日期：2006-02-10 00:00:00
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更新日期：1993-01-20 00:00:00
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pub_type： 杂志文章
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更新日期：1984-04-05 00:00:00
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更新日期：2008-10-03 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章,评审
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更新日期：2018-11-02 00:00:00
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pub_type： 杂志文章
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更新日期：2002-05-03 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：1994-03-18 00:00:00
abstract：:The structure of empty canine parvovirus capsids shows that residues 37 to the carboxy-terminal residue 584 (VP2 numbering) are ordered in each of the 60 subunits. The central structural motif of each subunit is the eight-stranded antiparallel beta-barrel that has been found in many other virus structures. Five beta-h...
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pub_type： 杂志文章
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更新日期：1993-09-20 00:00:00
