Structure of an archaeal RNA polymerase.

abstract：:The crystal structure of the eubacteria Mycoplasma genitalium ORF MG438 polypeptide, determined by multiple anomalous dispersion and refined at 2.3 A resolution, reveals the organization of S subunits from the Type I restriction and modification system. The structure consists of two globular domains, with about 150 re...

journal_title：Journal of molecular biology

authors： Calisto BM,Pich OQ,Piñol J,Fita I,Querol E,Carpena X

更新日期：2005-08-26 00:00:00

abstract：:Phosphofructokinase-1 and -2 (Pfk-1 and Pfk-2, respectively) from Escherichia coli belong to different homologous superfamilies. However, in spite of the lack of a common ancestor, they share the ability to catalyze the same reaction and are inhibited by the substrate MgATP. Pfk-2, an ATP-dependent 6-phosphofructokina...

journal_title：Journal of molecular biology

authors： Cabrera R,Ambrosio AL,Garratt RC,Guixé V,Babul J

更新日期：2008-11-14 00:00:00

abstract：:Recently, the absence of a thermally induced transition has been offered as proof that the unfolded and the molten globule states of apo-alpha-lactalbumin are enthalpically equivalent. In this paper we demonstrate that that argument is thermodynamically incorrect. In addition, it is shown that the absence of a thermal...

journal_title：Journal of molecular biology

authors： Xie D,Bhakuni V,Freire E

更新日期：1993-07-05 00:00:00

abstract：:Reverse transcriptase, an essential retroviral DNA polymerase, replicates the single-stranded RNA genome of the retrovirus, producing a double-stranded DNA copy, which is subsequently integrated into the host's genome. Substitution of Ala for either Asp114 or Arg116, two highly conserved residues in the fingers domain...

journal_title：Journal of molecular biology

authors： Gu J,Villanueva RA,Snyder CS,Roth MJ,Georgiadis MM

更新日期：2001-01-12 00:00:00

abstract：:The location of the single cysteinyl residue of the alkali light chain on the myosin head was determined by electron microscopy. The cysteinyl residue of isolated alkali light chain 2 was biotinylated and the light chain was exchanged with that of heavy meromyosin in 4.7 M-NH4Cl. Avidin was attached to the biotin in t...

journal_title：Journal of molecular biology

authors： Yamamoto K,Tokunaga M,Sutoh K,Wakabayashi T,Sekine T

更新日期：1985-05-25 00:00:00

abstract：:Kinetics, thermodynamics and structural aspects of human alpha-thrombin (thrombin) inhibition by newly synthesized low molecular weight derivatives of alpha-azalysine have been investigated. The thrombin catalyzed hydrolysis of N-ethoxycarbonyl-D-Phe-Pro-alpha-azaLys p-nitrophenyl ester (Eoc-D-Phe-Pro-azaLys-ONp) and ...

journal_title：Journal of molecular biology

authors： De Simone G,Balliano G,Milla P,Gallina C,Giordano C,Tarricone C,Rizzi M,Bolognesi M,Ascenzi P

更新日期：1997-06-20 00:00:00

abstract：:We have isolated and sequenced a cloned complementary DNA insert complementary to the messenger RNA of a cytoplasmic actin expressed in human epidermal cells. This provides the first cytoplasmic actin complementary DNA sequence for a vertebrate organism. The actin amino acid sequence predicted from this complementary ...

journal_title：Journal of molecular biology

authors： Hanukoglu I,Tanese N,Fuchs E

更新日期：1983-02-05 00:00:00

abstract：:While the gene for p53 is mutated in many human cancers causing loss of function, many others maintain a wild-type gene but exhibit reduced p53 tumor suppressor activity through overexpression of the negative regulators, Mdm2 and/or MdmX. For the latter mechanism of loss of function, the activity of endogenous p53 can...

journal_title：Journal of molecular biology

authors： Grace CR,Ban D,Min J,Mayasundari A,Min L,Finch KE,Griffiths L,Bharatham N,Bashford D,Kiplin Guy R,Dyer MA,Kriwacki RW

更新日期：2016-03-27 00:00:00

abstract：:In response to intracellular stress events ranging from starvation to pathogen invasion, the cell activates one or more forms of macroautophagy. The key event in these related pathways is the de novo formation of a new organelle called the autophagosome, which either surrounds and sequesters random portions of the cyt...

journal_title：Journal of molecular biology

authors： Nguyen N,Shteyn V,Melia TJ

更新日期：2017-02-17 00:00:00

abstract：:The structure of the Escherichia coli Dam DNA-(adenine-N6)-methyltransferase in complex with cognate DNA was determined at 1.89 A resolution in the presence of S-adenosyl-L-homocysteine. DNA recognition and the dynamics of base-flipping were studied by site-directed mutagenesis, DNA methylation kinetics and fluorescen...

journal_title：Journal of molecular biology

authors： Horton JR,Liebert K,Bekes M,Jeltsch A,Cheng X

更新日期：2006-04-28 00:00:00

abstract：:A multiple alignment of five (beta/alpha)8-barrel enzymes has been derived from their structure. The eight beta-strands and eight alpha-helices of the (beta/alpha)8-barrel are correctly aligned and the equivalenced residues in these regions fulfil similar structural roles. Each beta-strand has a central core of usuall...

journal_title：Journal of molecular biology

authors： Pickett SD,Saqi MA,Sternberg MJ

更新日期：1992-11-05 00:00:00

abstract：:Crystal structures of Bacillus stearothermophilus TRS40 neopullulanase and its complexes with panose, maltotetraose and isopanose were determined at resolutions of 1.9, 2.4, 2.8 and 3.2A, respectively. Since the latter two carbohydrates are substrates of this enzyme, a deactivated mutant at the catalytic residue Glu35...

journal_title：Journal of molecular biology

authors： Hondoh H,Kuriki T,Matsuura Y

更新日期：2003-02-07 00:00:00

abstract：:The family of fibroblast growth factor receptors (FGFRs) plays an important and well-characterized role in a variety of pathological disorders. FGFR4 is involved in myogenesis and muscle regeneration. Mutations affecting the kinase domain of FGFR4 may cause cancer, for example, breast cancer or rhabdomyosarcoma. Where...

journal_title：Journal of molecular biology

authors： Lesca E,Lammens A,Huber R,Augustin M

更新日期：2014-11-11 00:00:00

abstract：:Using HU chemical nucleases to probe HU-DNA interactions, we report here for the first time site-specific binding of HU to naked DNA. An unique feature of this interaction is the absolute requirement for negative DNA supercoiling for detectable levels of site-specific DNA binding. The HU binding site is the Mu spacer ...

journal_title：Journal of molecular biology

authors： Kobryn K,Lavoie BD,Chaconas G

更新日期：1999-06-18 00:00:00

abstract：:The Rev-erb family is an orphan nuclear receptor acting as a negative regulator of transcription. Rev-erbalpha and Rev-erbbeta are crucial components of the circadian clock and involved in various lipid homeostasis. They are unique nuclear receptors that lack the activation function 2 helix (AF2-helix) required for li...

journal_title：Journal of molecular biology

authors： Woo EJ,Jeong DG,Lim MY,Jun Kim S,Kim KJ,Yoon SM,Park BC,Ryu SE

更新日期：2007-10-26 00:00:00

abstract：:The vitamin D-binding protein, Gc, was purified from human serum and crystallized using the hanging-drop method. The best crystals were grown from 28% polyethylene glycol 400 in 50 mM-sodium acetate at pH 4.8. These crystals diffract to 3.4 A and the observed diffraction is consistent with orthorhombic space groups P4...

journal_title：Journal of molecular biology

authors： Vogelaar NJ,Lindberg U,Schutt CE

更新日期：1991-08-05 00:00:00

abstract：:Heme is an essential prosthetic group of proteins involved in oxygen transport, energy metabolism and nitric oxide production. ALAS1 (5-aminolevulinate synthase) is the rate-limiting enzyme in heme synthesis in the liver and is highly regulated to adapt to the metabolic demand of the hepatocyte. In the present study, ...

journal_title：Journal of molecular biology

authors： Degenhardt T,Väisänen S,Rakhshandehroo M,Kersten S,Carlberg C

更新日期：2009-05-01 00:00:00

abstract：:The crystal structure of a class A beta-lactamase from Staphylococcus aureus PC1 has been refined at 2.0 A resolution. The resulting crystallographic R-factor (R = sigma h parallel Fo[-]Fc parallel/sigma h[Fo], where [Fo] and [Fc] are the observed and calculated structure factor amplitudes, respectively), is 0.163 for...

journal_title：Journal of molecular biology

authors： Herzberg O

更新日期：1991-02-20 00:00:00

abstract：:Six R sequences have been described as members of a new family of dispersed repetitive DNA in the mouse genome (Gebhard et al., 1982). Several sequenced regions were extended in the 5' direction (R1, R2 and R6) and two new sequences were determined (R7 and R8). On the basis of our sequence and blot hybridization data ...

journal_title：Journal of molecular biology

authors： Gebhard W,Zachau HG

更新日期：1983-10-25 00:00:00

abstract：:The metazoan cell membrane is highly organized. Maintaining such organization and preserving membrane integrity under different conditions are accomplished through intracellular tethering to an extensive, flexible protein network. Spectrin, the principal component of this network, is attached to the membrane through t...

journal_title：Journal of molecular biology

authors： Yasunaga M,Ipsaro JJ,Mondragón A

更新日期：2012-04-06 00:00:00

abstract：:The loop between the F and G beta strands (FG loop) of the bacteriophage MS2 coat protein subunit forms inter-subunit contacts around the 5-fold and 3-fold (quasi 6-fold) axes of the T=3 protein shell. In capsids, the loop is found in two very different conformations, one in B subunits, which form the 5-fold contact, ...

journal_title：Journal of molecular biology

authors： Stonehouse NJ,Valegård K,Golmohammadi R,van den Worm S,Walton C,Stockley PG,Liljas L

更新日期：1996-02-23 00:00:00

abstract：:Translocation of the majority of mitochondrial proteins from the cytosol into mitochondria requires the cooperation of TOM and TIM23 complexes in the outer and inner mitochondrial membranes. The molecular mechanisms underlying this cooperation remain largely unknown. Here, we present biochemical and genetic evidence t...

journal_title：Journal of molecular biology

authors： Waegemann K,Popov-Čeleketić D,Neupert W,Azem A,Mokranjac D

更新日期：2015-03-13 00:00:00

abstract：:Escherichia coli lac promoter variants are shown to be subject to large synergistic transcriptional activation by catabolite activator protein (CRP) and DNA supercoiling in vitro. Activation was studied for the lac wild-type promoter, a promoter with a variant spacing (lac delta l) and two promoters with variant -10 r...

journal_title：Journal of molecular biology

authors： Meiklejohn AL,Gralla JD

更新日期：1989-06-20 00:00:00

abstract：:The protection of telomeres 1 (Pot1) proteins specifically recognize the single-stranded 3' end of the telomere, an activity essential for sustained cellular viability and proliferation. The current model for the telomeric single-stranded DNA (ssDNA) binding activity of Schizosaccharomyces pombe Pot1 is based on a 20 ...

journal_title：Journal of molecular biology

authors： Croy JE,Podell ER,Wuttke DS

更新日期：2006-08-04 00:00:00

abstract：:The periplasmic domain of the aspartate chemotaxis receptor from Salmonella typhimurium has been crystallized in the presence and absence of bound aspartate. Both crystal forms were grown by precipitation with lithium sulfate and diffract to 1.8 A resolution. The aspartate receptor structure is believed to be prototyp...

journal_title：Journal of molecular biology

authors： Jancarik J,Scott WG,Milligan DL,Koshland DE Jr,Kim SH

更新日期：1991-09-05 00:00:00

abstract：:The prediction of protein-protein interactions in solution is a major goal of theoretical structural biology. Here, we implement a continuum description of the thermodynamic processes involved. The model differs considerably from previous models in its use of "molecular surface" area to describe the hydrophobic compon...

journal_title：Journal of molecular biology

authors： Jackson RM,Sternberg MJ

更新日期：1995-07-07 00:00:00

abstract：:The catalytic pentad of tyrosine recombinases, that assists the tyrosine nucleophile, includes a conserved histidine/tryptophan (His/Trp-III). Flp and Cre harbor tryptophan at this position; most of their kin recombinases display histidine. Contrary to the conservation rule, Flp(W330F) is a much stronger recombinase t...

journal_title：Journal of molecular biology

authors： Ma CH,Kwiatek A,Bolusani S,Voziyanov Y,Jayaram M

更新日期：2007-04-20 00:00:00

abstract：:Protein engineering experiments and Phi(F)-value analysis of SH3 domains reveal that their transition state ensemble (TSE) is conformationally restricted, i.e. the fluctuations in the transition state (TS) structures are small. In the TS of src SH3 and alpha-spectrin SH3 the distal loop and the associated hairpin are ...

journal_title：Journal of molecular biology

authors： Klimov DK,Thirumalai D

更新日期：2002-04-12 00:00:00

abstract：:Severe thermal stress induces massive intracellular protein aggregation. The concerted action of Hsp70 (DnaK, DnaJ, GrpE) and Hsp100 (ClpB) chaperones results in solubilization of aggregates followed by reactivation of proteins. It was shown that the Hsp70 chaperone system works at the initial step of the disaggregati...

journal_title：Journal of molecular biology

authors： Lewandowska A,Matuszewska M,Liberek K

更新日期：2007-08-17 00:00:00

abstract：:Challenges in the production of integral membrane proteins for structural studies include low expression levels, incorrect membrane insertion, aggregation and instability. In this report, we describe a "funnel approach" to overcoming these difficulties and demonstrate its efficacy in a case study of 36 prokaryotic P-t...

journal_title：Journal of molecular biology

authors： Lewinson O,Lee AT,Rees DC

更新日期：2008-03-14 00:00:00