Crystallization and preliminary X-ray diffraction study of the ligand-binding domain of the bacterial chemotaxis-mediating aspartate receptor of Salmonella typhimurium.


:The periplasmic domain of the aspartate chemotaxis receptor from Salmonella typhimurium has been crystallized in the presence and absence of bound aspartate. Both crystal forms were grown by precipitation with lithium sulfate and diffract to 1.8 A resolution. The aspartate receptor structure is believed to be prototypical of a large class of receptors including those for polypeptide growth factor hormones as well as those for small chemotaxis-affector molecules such as aspartate and serine.


J Mol Biol


Jancarik J,Scott WG,Milligan DL,Koshland DE Jr,Kim SH





Has Abstract


1991-09-05 00:00:00














  • The effect of temperature on the structure of vinblastine-induced polymers of purified tubulin: detection of a reversible conformational change.

    abstract::Addition of the antimitotic drug vinblastine to solutions of purified tubulin induces the formation of helical polymers whose structure and type of aggregation is determined by the concentration of magnesium. While paracrystalline arrangements of single coils are observed at low concentrations of the ion, for concentr...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Nogales E,Medrano FJ,Diakun GP,Mant GR,Towns-Andrews E,Bordas J

    更新日期:1995-12-01 00:00:00

  • Structure-based analysis of GPCR function: evidence for a novel pentameric assembly between the dimeric leukotriene B4 receptor BLT1 and the G-protein.

    abstract::We produced human leukotriene B(4) (LTB(4)) receptor BLT1 as a recombinant protein in Escherichia coli. This detergent-solubilized receptor displays two states with regard to its affinity for LTB(4): (i) a low-affinity state (K(a)=7.8x10(8)M(-1)) that involves a receptor homodimer (BLT1.LTB(4))(2); we report evidence ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Banères JL,Parello J

    更新日期:2003-06-13 00:00:00

  • A thermophilic mini-chaperonin contains a conserved polypeptide-binding surface: combined crystallographic and NMR studies of the GroEL apical domain with implications for substrate interactions.

    abstract::A homologue of the Escherichia coli GroEL apical domain was obtained from thermophilic eubacterium Thermus thermophilus. The domains share 70 % sequence identity (101 out of 145 residues). The thermal stability of the T. thermophilus apical domain (Tm>100 degrees C as evaluated by circular dichroism) is at least 35 de...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Hua Q,Dementieva IS,Walsh MA,Hallenga K,Weiss MA,Joachimiak A

    更新日期:2001-02-23 00:00:00

  • The mannose-specific bulb lectin from Galanthus nivalis (snowdrop) binds mono- and dimannosides at distinct sites. Structure analysis of refined complexes at 2.3 A and 3.0 A resolution.

    abstract::Galanthus nivalis agglutinin (GNA, a 50 kDa tetramer) is a mannose-specific lectin of the Amaryllidaceae family of bulb lectins. Crystal structures of GNA complexed with methyl-alpha-D-mannose (MeMan) and mannose-alpha 1,3-D-mannose-alpha-OMe (MeMan-2) have been determined and analyzed in terms of internal structural ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Hester G,Wright CS

    更新日期:1996-10-04 00:00:00

  • Crystal structure of Bacillus sp. GL1 xanthan lyase complexed with a substrate: insights into the enzyme reaction mechanism.

    abstract::Bacillus sp. GL1 xanthan lyase, a member of polysaccharide lyase family 8 (PL-8), acts exolytically on the side-chains of pentasaccharide-repeating polysaccharide xanthan and cleaves the glycosidic bond between glucuronic acid (GlcUA) and pyruvylated mannose (PyrMan) through a beta-elimination reaction. To clarify the...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Maruyama Y,Hashimoto W,Mikami B,Murata K

    更新日期:2005-07-29 00:00:00

  • The effect of chloramphenicol in intact erythroid cells.

    abstract::Incubation of intact reticulocytes with chloramphenicol at concentrations over 10(-3) M caused inhibition of protein synthesis and conversion of polyribosomes to single ribosomes. At 3 x 10(-3) M-chloramphenicol these effects were completely reversible, but reversibility decreased as the chloramphenicol concentration ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Godchaux W 3rd,Herbert E

    更新日期:1966-11-28 00:00:00

  • Reverse turns in blocked dipeptides are intrinsically unstable in water.

    abstract::We have carried out molecular dynamics simulations to study the conformational equilibria of two blocked dipeptides, Ac-Ala-Ala-NHMe and trans-Ac-Pro-Ala-NHMe, in water (Ac, amino-terminal blocking group COCH3; NHMe, carboxy-terminal blocking group NHCH3). Using specialized sampling techniques we computed free-energy ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Tobias DJ,Sneddon SF,Brooks CL 3rd

    更新日期:1990-12-05 00:00:00

  • X-ray analyses of aspartic proteinases. V. Structure and refinement at 2.0 A resolution of the aspartic proteinase from Mucor pusillus.

    abstract::The structure of mucor pusillus pepsin (EC, the aspartic proteinase from Mucor pusillus, has been refined to a crystallographic R-factor of 16.2% at 2.0 A resolution. The positions of 2638 protein atoms, 221 solvent atoms and a sulphate ion have been determined with an estimated root-mean-square (r.m.s.) err...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Newman M,Watson F,Roychowdhury P,Jones H,Badasso M,Cleasby A,Wood SP,Tickle IJ,Blundell TL

    更新日期:1993-03-05 00:00:00

  • Crystallization and preliminary crystallographic study of cytochrome cd1 nitrite reductase from Thiosphaera pantotropha.

    abstract::Single crystals of cytochrome cd1 nitrite reductase from Thiosphaera pantotropha have been grown in the presence of ammonium sulphate by vapour diffusion techniques. Crystals are suitable for X-ray analysis and diffract to dmin = 1.5 A. They belong to space group P2(1) with unit cell dimensions of a = 107.8 A, b = 61....

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Fülöp V,Moir JW,Ferguson SJ,Hajdu J

    更新日期:1993-08-20 00:00:00

  • Molecular Basis of Class III Ligand Recognition by PDZ3 in Murine Protein Tyrosine Phosphatase PTPN13.

    abstract::Protein tyrosine phosphatase PTPN13, also known as PTP-BL in mice, represents a large multi-domain non-transmembrane scaffolding protein that contains five consecutive PDZ domains. Here, we report the solution structures of the extended murine PTPN13 PDZ3 domain in its apo form and in complex with its physiological li...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Kock G,Dicks M,Yip KT,Kohl B,Pütz S,Heumann R,Erdmann KS,Stoll R

    更新日期:2018-10-19 00:00:00

  • Fluid and condensed ApoA-I/phospholipid monolayers provide insights into ApoA-I membrane insertion.

    abstract::Apolipoprotein A-I (ApoA-I) is a protein implicated in the solubilization of lipids and cholesterol from cellular membranes. The study of ApoA-I in phospholipid (PL) monolayers brings relevant information about ApoA-I/PL interactions. We investigated the influence of PL charge and acyl chain organization on the intera...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Chièze L,Bolanos-Garcia VM,Pinot M,Desbat B,Renault A,Beaufils S,Vié V

    更新日期:2011-07-01 00:00:00

  • Oxygen transport by fetal bovine hemoglobin.

    abstract::The functional properties of fetal bovine hemoglobin have been studied as a function of temperature, chloride and 2,3-diphosphoglycerate (DPG) concentration. The fetal bovine erythrocyte has six times the concentration of the allosteric modulator DPG compared with the adult cell, and yet the oxygen affinity of the fet...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Clementi ME,Scatena R,Mordente A,Condò SG,Castagnola M,Giardina B

    更新日期:1996-01-12 00:00:00

  • Genetic interactions among the transmembrane segments of the G protein coupled receptor encoded by the yeast STE2 gene.

    abstract::G protein coupled receptors (GPCRs) are integral membrane proteins that mediate cellular responses to a wide variety of extracellular signals. However, the structural basis for activation of this class of receptors by ligand binding is not well understood. We report here the use of a systematic genetic protocol for id...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Sommers CM,Dumont ME

    更新日期:1997-02-28 00:00:00

  • Specificity in protein-DNA interactions: energetic recognition by the (cytosine-C5)-methyltransferase from HhaI.

    abstract::Sequence-specific interactions between proteins and DNA are essential for a variety of biological functions. The (cytosine-C5)-methyltransferase from HhaI (M.HhaI) specifically modifies the second base in GCGC sequences, employing a base flipping mechanism to access the target base being chemically modified. The mecha...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Huang N,MacKerell AD Jr

    更新日期:2005-01-14 00:00:00

  • X-ray structure analysis and crystallographic refinement of lumazine synthase from the hyperthermophile Aquifex aeolicus at 1.6 A resolution: determinants of thermostability revealed from structural comparisons.

    abstract::An open reading frame optimized for expression of 6,7-dimethyl-8-ribityl-lumazine synthase of the hyperthermophilic bacterium Aquifex aeolicus in Escherichia coli was synthesized and expressed in a recombinant E. coli strain to a level of around 15 %. The recombinant protein was purified by heat-treatment and gel-filt...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Zhang X,Meining W,Fischer M,Bacher A,Ladenstein R

    更新日期:2001-03-09 00:00:00

  • Functional analysis of the glucocorticoid regulatory elements present in the mouse mammary tumor virus long terminal repeat. A synthetic distal binding site can replace the proximal binding domain.

    abstract::Transcription of mouse mammary tumor virus DNA is stimulated by steroid hormones. The DNA sequences involved in this regulation are located in the viral long terminal repeat between positions -200 and -50 with respect to the transcription initiation site. In this region four, one distal and three proximal, in vitro bi...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Kühnel B,Buetti E,Diggelmann H

    更新日期:1986-08-05 00:00:00

  • Leishmania mexicana glycerol-3-phosphate dehydrogenase showed conformational changes upon binding a bi-substrate adduct.

    abstract::Certain pathogenic trypanosomatids are highly dependent on glycolysis for ATP production, and hence their glycolytic enzymes, including glycerol-3-phosphate dehydrogenase (GPDH), are considered attractive drug targets. The ternary complex structure of Leishmania mexicana GPDH (LmGPDH) with dihydroxyacetone phosphate (...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Choe J,Guerra D,Michels PA,Hol WG

    更新日期:2003-05-30 00:00:00

  • X-ray analysis of substrate analogs in the ricin A-chain active site.

    abstract::Ricin A-chain is an N-glycosidase that hydrolyzes the adenine ring from a specific adenosine of rRNA. Formycin monophosphate (FMP) and adenyl(3'-->5')guanosine (ApG) were bound to ricin A-chain and their structures elucidated by X-ray crystallography. The formycin ring stacks between tyrosines 80 and 123 and at least ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Monzingo AF,Robertus JD

    更新日期:1992-10-20 00:00:00

  • Long-range electrostatic interactions influence the orientation of Fos-Jun binding at AP-1 sites.

    abstract::Heterodimeric transcription regulatory proteins that bind palindromic DNA sequences can potentially bind their recognition sites in two opposite orientations. The orientation of transcription factor binding can control transcriptional activity by altering interactions with proteins that bind to adjacent regulatory ele...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Ramirez-Carrozzi VR,Kerppola TK

    更新日期:2001-01-19 00:00:00

  • The major human structural IgE epitope of the Brazil nut allergen Ber e 1: a chimaeric and protein microarray approach.

    abstract::A protein microarray system containing different dilutions of 77 related and non-related proteins was used to show that IgE from subjects allergic to Brazil nut specifically recognise the seed 2S albumin protein (Ber e 1). Further, correctly folded chimaeric 2S albumin proteins containing structural epitope replacemen...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Alcocer MJ,Murtagh GJ,Wilson PB,Progias P,Lin J,Archer DB

    更新日期:2004-10-22 00:00:00

  • Invertebrate myosin filament: subfilament arrangement in the wall of tubular filaments of insect flight muscles.

    abstract::Transverse sections (100 to 140 nm thick) of the flight muscles of the fleshfly Phormia terrae-novae and the housefly Musca domestica were studied. The images of 56 tubular myosin filaments of the fleshfly and 62 filaments of the housefly were digitized and computer processed by rotational averaging. The rotational po...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Beinbrech G,Ashton FT,Pepe FA

    更新日期:1988-06-05 00:00:00

  • Conformation of [Cd7]-metallothionein-2 from rat liver in aqueous solution determined by nuclear magnetic resonance spectroscopy.

    abstract::The three-dimensional structure of [Cd7]-metallothionein-2 from rat liver was determined in aqueous solution, using nuclear magnetic resonance spectrometry and distance geometry calculations. The experimental data provided proton-proton distance constraints from measurements of nuclear Overhauser effects, constraints ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Schultze P,Wörgötter E,Braun W,Wagner G,Vasák M,Kägi JH,Wüthrich K

    更新日期:1988-09-05 00:00:00

  • The folding energy landscape of the dimerization domain of Escherichia coli Trp repressor: a joint experimental and theoretical investigation.

    abstract::Enhanced structural insights into the folding energy landscape of the N-terminal dimerization domain of Escherichia coli tryptophan repressor, [2-66]2 TR, were obtained from a combined experimental and theoretical analysis of its equilibrium folding reaction. Previous studies have shown that the three intertwined heli...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Simler BR,Levy Y,Onuchic JN,Matthews CR

    更新日期:2006-10-13 00:00:00

  • Impact of the terminal bulges of HIV-1 cTAR DNA on its stability and the destabilizing activity of the nucleocapsid protein NCp7.

    abstract::Reverse transcription of HIV-1 genomic RNA to double-stranded DNA by reverse transcriptase (RT) is a critical step in HIV-1 replication. This process relies on two viral proteins, the RT enzyme and nucleocapsid protein NCp7 that has well documented nucleic acid chaperone properties. At the beginning of the linear DNA ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Beltz H,Azoulay J,Bernacchi S,Clamme JP,Ficheux D,Roques B,Darlix JL,Mély Y

    更新日期:2003-04-18 00:00:00

  • Crystallization and preliminary X-ray studies of a protease from Pseudomonas aeruginosa.

    abstract::A protease produced by Pseudomonas aeruginosa has been crystallized by the vapor-diffusion method using polyethylene glycol 4000 as a precipitant. The crystals belong to the hexagonal space group P6(1) (P6(5) with unit cell dimensions; a = b = 106.9 A, c = 96.9 A. There are two molecules per asymmetric unit. The cryst...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Harada S,Nagara A,Kurisu G,Kai Y

    更新日期:1993-04-20 00:00:00

  • Direct phage to intrabody screening (DPIS): demonstration by isolation of cytosolic intrabodies against the TES1 site of Epstein Barr virus latent membrane protein 1 (LMP1) that block NF-kappaB transactivation.

    abstract::The expression of intracellular antibodies (intrabodies) in eukaryotic cells has provided a powerful tool to manipulate microbial and cellular signaling pathways in a highly precise manner. However, there have been several technical issues that have restricted their more widespread use. In particular, single-chain ant...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Gennari F,Mehta S,Wang Y,St Clair Tallarico A,Palu G,Marasco WA

    更新日期:2004-01-02 00:00:00

  • The structure of Mycobacterium tuberculosis MPT51 (FbpC1) defines a new family of non-catalytic alpha/beta hydrolases.

    abstract::Mycobacterium tuberculosis, the causative agent of tuberculosis, is known to secrete a number of highly immunogenic proteins that are thought to confer pathogenicity, in part, by mediating binding to host tissues. Among these secreted proteins are the trimeric antigen 85 (Ag85) complex and the related MPT51 protein, a...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Wilson RA,Maughan WN,Kremer L,Besra GS,Fütterer K

    更新日期:2004-01-09 00:00:00

  • Genetic selection for critical residues in ribonucleases.

    abstract::Homologous mammalian proteins were subjected to an exhaustive search for residues that are critical to their structure/function. Error-prone polymerase chain reactions were used to generate random mutations in the genes of bovine pancreatic ribonuclease (RNase A) and human angiogenin, and a genetic selection based on ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Smith BD,Raines RT

    更新日期:2006-09-22 00:00:00

  • Sequence dependence of amyloid fibril formation: insights from molecular dynamics simulations.

    abstract::The clarification of the physico-chemical determinants underlying amyloid deposition is critical for our understanding of misfolding diseases. With this purpose we have performed a systematic all-atom molecular dynamics (MD) study of a series of single point mutants of the de novo designed amyloidogenic peptide STVIIE...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: López de la Paz M,de Mori GM,Serrano L,Colombo G

    更新日期:2005-06-10 00:00:00

  • Beta-propeller crystal structure of Psathyrella velutina lectin: an integrin-like fungal protein interacting with monosaccharides and calcium.

    abstract::The lectin from the mushroom Psathyrella velutina recognises specifically N-acetylglucosamine and N-acetylneuraminic acid containing glycans. The crystal structure of the 401 amino acid residue lectin shows that it adopts a very regular seven-bladed beta-propeller fold with the N-terminal region tucked into the centra...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Cioci G,Mitchell EP,Chazalet V,Debray H,Oscarson S,Lahmann M,Gautier C,Breton C,Perez S,Imberty A

    更新日期:2006-04-14 00:00:00