Crystallization and preliminary X-ray diffraction study of the ligand-binding domain of the bacterial chemotaxis-mediating aspartate receptor of Salmonella typhimurium.

abstract：:Rabbit psoas muscle filaments, isolated in relaxing buffer from non-glycerinated muscle, have been applied to hydrophilic carbon films and stained with uranyl acetate. Electron micrographs were obtained under low-dose conditions to minimize specimen damage. Surrounding the filament backbone, except in the bare zone, i...

journal_title：Journal of molecular biology

authors： Knight P,Trinick J

更新日期：1984-08-15 00:00:00

abstract：:Bacteriophage with double-stranded, linear DNA genomes package DNA into pre-assembled icosahedral procapsids through a unique vertex. The packaging vertex contains an oligomeric ring of a portal protein that serves as a recognition site for the packaging enzymes, a conduit for DNA translocation, and the site of tail a...

journal_title：Journal of molecular biology

authors： Moore SD,Prevelige PE Jr

更新日期：2002-02-01 00:00:00

abstract：:Recombinant expression systems differ in the type of glycosylation they impart on expressed antigens such as the human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins, potentially affecting their biological properties. We performed head-to-head antigenic, immunogenic and molecular profiling of two distant...

journal_title：Journal of molecular biology

authors： Kong L,Sheppard NC,Stewart-Jones GBE,Robson CL,Chen H,Xu X,Krashias G,Bonomelli C,Scanlan CN,Kwong PD,Jeffs SA,Jones IM,Sattentau QJ

更新日期：2010-10-15 00:00:00

abstract：:phi 6 is an enveloped dsRNA bacterial virus. Its segmented genome resides inside the virion associated polymerase complex which is formed by four proteins (P1, P2, P4 and P7) encoded by the viral L segment. Complete and incomplete polymerase complex particles can be produced using cDNA copies of this largest genome se...

journal_title：Journal of molecular biology

authors： Juuti JT,Bamford DH

更新日期：1995-06-09 00:00:00

abstract：:The serendipitous observation of a C-H cdots, three dots, centered O hydrogen bond mediated polypeptide chain reversal in synthetic peptide helices has led to a search for the occurrence of a similar motif in protein structures. From a dataset of 634 proteins, 1304 helices terminating in a Schellman motif have been ex...

journal_title：Journal of molecular biology

authors： Madan Babu M,Kumar Singh S,Balaram P

更新日期：2002-09-27 00:00:00

abstract：:We demonstrate here that the Saccharomyces cerevisiae Mlh1-Pms1 heterodimer required for DNA mismatch repair and other cellular processes is a DNA binding protein. Binding was evaluated using a variety of single and double-stranded DNA molecules. Mlh1-Pms1 bound short substrates with low affinity and showed a slight p...

journal_title：Journal of molecular biology

authors： Hall MC,Wang H,Erie DA,Kunkel TA

更新日期：2001-09-28 00:00:00

abstract：:Herpesvirus proteases are essential for the production of progeny virus. They cleave the assembly protein that fills the immature capsid in order to make place for the viral DNA. The recombinant protease of the human gamma-herpesvirus Epstein-Barr virus (EBV) was expressed in Escherichia coli and purified. Circular di...

journal_title：Journal of molecular biology

authors： Buisson M,Valette E,Hernandez JF,Baudin F,Ebel C,Morand P,Seigneurin JM,Arlaud GJ,Ruigrok RW

更新日期：2001-08-03 00:00:00

abstract：:Transmembrane proteins (TMPs) play important roles in many biological processes, such as cell recognition and communication. Their structures are crucial for revealing complex functions but are hard to obtain. A variety of computational algorithms have been proposed to fill the gap by predicting structures from primar...

journal_title：Journal of molecular biology

authors： Feng SH,Zhang WX,Yang J,Yang Y,Shen HB

更新日期：2020-02-14 00:00:00

abstract：:At high concentrations, type I pN-collagen, pC-collagen and procollagen (the first 2 generated from procollagen by enzymic cleavage of C-propeptides and N-propeptides, respectively) can all be made to assemble in vitro into thin D-periodic sheets or tapes. Scanning transmission electron microscopy mass measurements sh...

journal_title：Journal of molecular biology

authors： Holmes DF,Mould AP,Chapman JA

更新日期：1991-07-05 00:00:00

abstract：:The MotA protein of Escherichia coli is a component of the flagellum that functions, together with MotB, in transmembrane proton conduction. MotA and MotB are believed to form the stator of the flagellar motor. They are integral membrane proteins; MotA has a large (ca 22 kDa) domain in the cytoplasm, and MotB a much s...

journal_title：Journal of molecular biology

authors： Zhou J,Blair DF

更新日期：1997-10-24 00:00:00

abstract：:Human RNA polymerase II recognizes a strong transcriptional pause signal in the initially transcribed region of HIV-1. We report the use of a limited-step transcription assay to dissect the mechanism underlying recognition of and escape from this HIV-1 pause. Our results suggest that the primary determinant of transcr...

journal_title：Journal of molecular biology

authors： Palangat M,Landick R

更新日期：2001-08-10 00:00:00

abstract：:Anterior gradient-2 (AGR2) functions in a range of biological systems, including goblet cell formation, limb regeneration, inhibition of p53, and metastasis. There are no well-validated binding proteins for AGR2 protein despite the wealth of data implicating an important cellular function in vertebrates. The yeast two...

journal_title：Journal of molecular biology

authors： Maslon MM,Hrstka R,Vojtesek B,Hupp TR

更新日期：2010-12-03 00:00:00

abstract：:The C repeat family was first observed in the rabbit beta-like gene family. We have estimated the repetition frequency of the C repeats, determined the nucleotide sequence of three intact members and one truncated member, and have investigated the size, tissue specificity, and intracellular localization of C repeat tr...

journal_title：Journal of molecular biology

authors： Cheng JF,Printz R,Callaghan T,Shuey D,Hardison RC

更新日期：1984-06-15 00:00:00

abstract：:By expressing a mutant trpR gene in an Escherichia coli strain that is trpR and has beta-galactosidase activity fused to the trp promoter/operator, thus putting the beta-galactosidase activity under the control of the Trp repressor, we can determine quantitatively the relative repression activity of such mutant(s). We...

journal_title：Journal of molecular biology

authors： Storbakk N,Fenton C,Riise HM,Nilsen IW,El-Gewely MR

更新日期：1996-03-15 00:00:00

abstract：:The 90-kDa heat shock proteins (Hsp90s) assist the maturation of many key regulators of signal transduction pathways and cellular control circuits like protein kinases and transcription factors and chaperone their stability and activity. In this function, Hsp90s cooperate with some 30 cochaperones and they are themsel...

journal_title：Journal of molecular biology

authors： Nguyen MTN,Knieß RA,Daturpalli S,Le Breton L,Ke X,Chen X,Mayer MP

更新日期：2017-03-10 00:00:00

abstract：:The N-terminal segment (FR-H1) of the heavy chain (V(H)) of antibodies shows significant conformational variability correlating with the nature of the amino acids H6, H7 and H10 (Kabat H9). In this study, we have established a causal relationship between the local sequence and the structure of this framework region an...

journal_title：Journal of molecular biology

authors： Jung S,Spinelli S,Schimmele B,Honegger A,Pugliese L,Cambillau C,Plückthun A

更新日期：2001-06-08 00:00:00

abstract：:Binding of the histone (H2A, H2B) dimer with chicken erythrocyte DNA has been studied by salt-titration spectroscopy in equilibrium conditions. The circular dichroism of DNA near 275 nm is depressed by the interaction with (H2A, H2B) at low concentrations of salt. The depression increases with increasing amounts of (H...

journal_title：Journal of molecular biology

authors： Oohara I,Wada A

更新日期：1987-07-20 00:00:00

abstract：:The NusA transcription elongation protein, which binds RNA, contains sequences corresponding to the S1 and KH classes of identified RNA binding domains. An essential function in E. coli, NusA is also one of the host factors required for action of the N transcription antitermination protein of lambda. Tandem KH domains...

journal_title：Journal of molecular biology

authors： Zhou Y,Mah TF,Greenblatt J,Friedman DI

更新日期：2002-05-17 00:00:00

abstract：:"Positive control" mutants of the cI protein of bacteriophage lambda (lambda cI) bind DNA but, unlike the wild-type protein, fail to activate transcription. According to the original interpretation of Ptashne and co-workers, these mutants bear amino acid substitutions that disrupt a stimulatory interaction between lam...

journal_title：Journal of molecular biology

authors： Whipple FW,Ptashne M,Hochschild A

更新日期：1997-01-24 00:00:00

abstract：:Epistasis is a key factor in evolution since it determines which combinations of mutations provide adaptive solutions and which mutational pathways toward these solutions are accessible by natural selection. There is growing evidence for the pervasiveness of sign epistasis--a complete reversion of mutational effects, ...

journal_title：Journal of molecular biology

authors： Dellus-Gur E,Elias M,Caselli E,Prati F,Salverda ML,de Visser JA,Fraser JS,Tawfik DS

更新日期：2015-07-17 00:00:00

abstract：:Single crystals of ribonuclease I from Escherichia coli have been obtained by the vapor diffusion method. The crystals belong to the tetragonal space group P4(1)2(1)2 or its enantiomer P4(3)2(1)2 and have cell parameters a = b = 119.01 A and c = 34.40 A. There is one 27,000 dalton monomer in the asymmetric unit. The c...

journal_title：Journal of molecular biology

authors： Lim LW,Mathur S,Cannistraro VJ,Kennell D

更新日期：1993-11-20 00:00:00

abstract：:Polyglutamine (polyQ) amyloid fibrils are observed in disease tissue and have been implicated as toxic agents responsible for neurodegeneration in expanded CAG repeat diseases such as Huntington's disease. Despite intensive efforts, the mechanism of amyloid toxicity remains unknown. As a novel approach to probing poly...

journal_title：Journal of molecular biology

authors： Kar K,Arduini I,Drombosky KW,van der Wel PC,Wetzel R

更新日期：2014-02-20 00:00:00

abstract：:Microtubule (MT) stability is related to the hydrolysis of the guanosine triphosphate nucleotide (NT) bound to β-tubulin. However, the molecular mechanism by which the NT state influences the stability of the contacts in the MT lattice remains elusive. Here, we present large-scale atomistic simulations of different tu...

journal_title：Journal of molecular biology

authors： Grafmüller A,Noya EG,Voth GA

更新日期：2013-06-26 00:00:00

abstract：:The type A domain of the von Willebrand Factor is found also in the complement proteins factor B (FB), C2, CR3 and CR4, the integrins, collagen types VI, VII, XII and XIV, and other proteins. FB is a component of the alternative pathway of the complement system of immune defence, and is cleaved into the fragments Bb a...

journal_title：Journal of molecular biology

authors： Perkins SJ,Smith KF,Williams SC,Haris PI,Chapman D,Sim RB

更新日期：1994-04-22 00:00:00

abstract：:Kinetic and crystallographic studies have characterized the effect of 2-deoxy-glucose 6-phosphate on the catalytic and structural properties of glycogen phosphorylase b. Previous work on the binding of glucose 6-phosphate, a potent physiological inhibitor of the enzyme, to T state phosphorylase b in the crystal showed...

journal_title：Journal of molecular biology

authors： Oikonomakos NG,Zographos SE,Johnson LN,Papageorgiou AC,Acharya KR

更新日期：1995-12-15 00:00:00

abstract：:We have studied the reversible dissociation of core size DNA from chicken erythrocyte nucleosome core particles in solutions containing 0 X 1 M to 0 X 6 M-NaCl. Dissociation increases with increasing NaCl concentration, increasing temperature and decreasing particle concentration. At high particle concentrations, no f...

journal_title：Journal of molecular biology

authors： Ausio J,Seger D,Eisenberg H

更新日期：1984-06-15 00:00:00

abstract：:A simple continuum model of a de novo designed model of a four-helix bundle is presented. The thermodynamics and kinetics of the model are studied using Langevin simulations. We use a three-letter minimal off-lattice representation of a de novo designed four-helix bundle protein. The native state of the model, which c...

journal_title：Journal of molecular biology

authors： Guo Z,Thirumalai D

更新日期：1996-10-25 00:00:00

abstract：:Restriction endonuclease BcnI cleaves duplex DNA containing the sequence CC/SGG (S stands for C or G, / designates a cleavage position) to generate staggered products with single nucleotide 5'-overhangs. Here, we show that BcnI functions as a monomer that interacts with its target DNA in 1:1 molar ratio and report cry...

journal_title：Journal of molecular biology

authors： Sokolowska M,Kaus-Drobek M,Czapinska H,Tamulaitis G,Szczepanowski RH,Urbanke C,Siksnys V,Bochtler M

更新日期：2007-06-08 00:00:00

abstract：:The backbone dynamics of the EF-hand Ca(2+)-binding protein, calbindin D9k, has been investigated in the apo, (Cd2+)1 and (Ca2+)2 states by measuring the rate constants for amide proton exchange with solvent. 15N-1H correlation spectroscopy was utilized to follow direct 1H-->2H exchange of the slowly exchanging amide ...

journal_title：Journal of molecular biology

authors： Skelton NJ,Kördel J,Akke M,Chazin WJ

更新日期：1992-10-20 00:00:00

abstract：:FGF21 is a member of a unique subfamily of fibroblast growth factors that function as endocrine hormones and regulate a variety of metabolic activities. Unlike paracrine FGFs, FGF21 does not bind heparin and requires βKlotho as a co-receptor to activate FGFR signaling. In the presence of βKlotho, FGF21 is able to acti...

journal_title：Journal of molecular biology

authors： Gupte J,Yang L,Wu X,Weiszmann J,Hecht R,Lemon B,Lindberg R,Wang Z,Li Y

更新日期：2011-05-06 00:00:00