Crystallization and preliminary X-ray analysis of Gc, the vitamin D-binding protein in serum.

abstract：:Normal type I collagen is a heterotrimer of two alpha1(I) and one alpha2(I) chains, but various genetic and environmental factors result in synthesis of homotrimers that consist of three alpha1(I) chains. The homotrimers completely replace the heterotrimers only in rare recessive disorders. In the general population, ...

journal_title：Journal of molecular biology

authors： Han S,McBride DJ,Losert W,Leikin S

更新日期：2008-10-31 00:00:00

abstract：:The contribution of the tRNA "core" to aminoacylation is beginning to be recognized. One example is the core region of Escherichia coli tRNA(Cys), which has been shown by biochemical studies to be important for aminoacylation. This core has several layers of unusual base-pairs, which are revealed by the recent crystal...

journal_title：Journal of molecular biology

authors： Hamann CS,Hou YM

更新日期：2000-01-28 00:00:00

abstract：:The Gram-negative organism Campylobacter jejuni is the major cause of food poisoning. Unlike Escherichia coli, which has two major porins, OmpC and OmpF, C. jejuni has one, termed major outer membrane protein (MOMP) through which nutrients and antibiotics transit. We report the 2.1-Å crystal structure of C. jejuni MOM...

journal_title：Journal of molecular biology

authors： Ferrara LGM,Wallat GD,Moynié L,Dhanasekar NN,Aliouane S,Acosta-Gutiérrez S,Pagès JM,Bolla JM,Winterhalter M,Ceccarelli M,Naismith JH

更新日期：2016-11-06 00:00:00

abstract：:The regulation of disulphide stress in actinomycetes such as Streptomyces coelicolor is known to involve the zinc-containing anti-sigma factor RsrA that binds and inactivates the redox-regulated sigma factor sigmaR. However, it is not known how RsrA senses disulphide stress nor what role the metal ion plays. Using in ...

journal_title：Journal of molecular biology

authors： Li W,Bottrill AR,Bibb MJ,Buttner MJ,Paget MS,Kleanthous C

更新日期：2003-10-17 00:00:00

abstract：:Bloom syndrome protein forms an oligomeric ring structure and belongs to a group of DNA helicases showing extensive homology to the Escherichia coli DNA helicase RecQ, a suppressor of illegitimate recombination. After over-production in E.coli, we have purified the RecQ core of BLM consisting of the DEAH, RecQ-Ct and ...

journal_title：Journal of molecular biology

authors： Janscak P,Garcia PL,Hamburger F,Makuta Y,Shiraishi K,Imai Y,Ikeda H,Bickle TA

更新日期：2003-06-27 00:00:00

abstract：:The evolution of new or improved enzyme specificities in prokaryotes has been proposed to involve gene duplication, followed by silencing of one of the duplicates at the transcriptional or translational level. Such "silent gene intermediates" are distinct from "cryptic" genes, which are proposed to have a different ro...

journal_title：Journal of molecular biology

authors： Burns DM,Beacham IR

更新日期：1986-11-20 00:00:00

abstract：:The TOPRIM domain found in many nucleotidyl transferases contains a DxD motif involved in magnesium ion coordination for catalysis. Medium- to high-copy-number plasmid clones of Yersinia pestis topoisomerase I (YpTOP) with Asp-to-Asn substitution at the first aspartate residue (D117N) of this motif could not be genera...

journal_title：Journal of molecular biology

authors： Cheng B,Annamalai T,Sorokin E,Abrenica M,Aedo S,Tse-Dinh YC

更新日期：2009-01-16 00:00:00

abstract：:The formation of two-dimensional crystals of the membrane-bound enzyme microsomal glutathione transferase is sensitive to fractional changes in the lipid-to-protein ratio. Variation of this parameter results in crystal polymorphism. The projection structure of a p6 crystal form of the enzyme has been determined by the...

journal_title：Journal of molecular biology

authors： Schmidt-Krey I,Murata K,Hirai T,Mitsuoka K,Cheng Y,Morgenstern R,Fujiyoshi Y,Hebert H

更新日期：1999-04-30 00:00:00

abstract：:DNase I digestion of unlabelled chromatin has been used to determine the orientation of nucleosomes in the higher-order structure of chromatin. DNA digestion patterns were analysed quantitatively and compared with simulation curves that were generated with the experimentally obtained rate constants for cutting inside ...

journal_title：Journal of molecular biology

authors： Staynov DZ,Proykova YG

更新日期：1998-05-29 00:00:00

abstract：:Bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) is an important intracellular signaling molecule that affects diverse physiological processes in bacteria. The intracellular levels of c-di-GMP are controlled by proteins acting as diguanylate cyclase (DGC) and phosphodiesterase (PDE) enzymes that synthesiz...

journal_title：Journal of molecular biology

authors： Pallegar P,Canuti M,Langille E,Peña-Castillo L,Lang AS

更新日期：2020-08-07 00:00:00

abstract：:Many viruses employ ATP-powered motors for genome packaging. We combined genetic, biochemical, and single-molecule techniques to confirm the predicted Walker-B ATP-binding motif in the phage λ motor and to investigate the roles of the conserved residues. Most changes of the conserved hydrophobic residues resulted in >...

journal_title：Journal of molecular biology

authors： delToro D,Ortiz D,Ordyan M,Pajak J,Sippy J,Catala A,Oh CS,Vu A,Arya G,Smith DE,Catalano CE,Feiss M

更新日期：2019-11-08 00:00:00

abstract：:The presence of bound water in the solution structure of the IgG binding domain of streptococcal protein G has been investigated by nuclear magnetic resonance using three-dimensional 1H rotating frame Overhauser 1H-15N multiple quantum coherence spectroscopy. The backbone amide protons of three residues, Ala20, Gln32 ...

journal_title：Journal of molecular biology

authors： Clore GM,Gronenborn AM

更新日期：1992-02-20 00:00:00

abstract：:Export of protein into the periplasm of Escherichia coli via the general secretory system is achieved by action of a ternary complex comprising the polypeptide ligand, the chaperone SecB and SecA, a peripheral component of the membrane translocon, which is itself an ATPase. The unfolded ligand is captured initially by...

journal_title：Journal of molecular biology

authors： Crane JM,Mao C,Lilly AA,Smith VF,Suo Y,Hubbell WL,Randall LL

更新日期：2005-10-21 00:00:00

abstract：:The active site of influenza virus neuraminidase (NA) is formed by 11 universally conserved residues. A guanidino group incorporated into two unrelated NA inhibitors was previously reported to occupy different negatively charged sites in the NA active site, A new inhibitor containing two guanidino groups was synthesiz...

journal_title：Journal of molecular biology

authors： Sudbeck EA,Jedrzejas MJ,Singh S,Brouillette WJ,Air GM,Laver WG,Babu YS,Bantia S,Chand P,Chu N,Montgomery JA,Walsh DA,Luo M

更新日期：1997-04-04 00:00:00

abstract：:Numerous experiments demonstrate a high level of promiscuity and structural disorder in organismal proteomes. Here, we ask the question what makes a protein promiscuous, that is, prone to nonspecific interactions, and structurally disordered. We predict that multi-scale correlations of amino acid positions within prot...

journal_title：Journal of molecular biology

authors： Afek A,Shakhnovich EI,Lukatsky DB

更新日期：2011-06-10 00:00:00

abstract：:Solution NMR structures for sperm whale carbonmonoxy myoglobin have been calculated using 1301 distance restraints determined from nuclear Overhauser enhancement (NOE) measurements on 15N-labeled protein and chemical shift calculations for 385 protons. Starting structures included four crystal forms of myoglobin and 1...

journal_title：Journal of molecular biology

authors： Osapay K,Theriault Y,Wright PE,Case DA

更新日期：1994-11-25 00:00:00

abstract：:The expression of the gene thrS encoding threonyl-tRNA synthetase is under the control of two apparently different regulatory loops: translational feedback regulation and growth rate-dependent control. The translational feedback regulation is due to the binding of threonyl-tRNA synthetase to a site located in the lead...

journal_title：Journal of molecular biology

authors： Comer MM,Dondon J,Graffe M,Yarchuk O,Springer M

更新日期：1996-08-16 00:00:00

abstract：:The structures of transition states and intermediates in protein folding may be analysed by protein engineering methods that remove simple interactions that stabilize the folded state. We have now extended the range and reliability of the procedure by using the COSMIC (Combination of Sequential Mutant Interaction Cycl...

journal_title：Journal of molecular biology

authors： Horovitz A,Serrano L,Fersht AR

更新日期：1991-05-05 00:00:00

abstract：:Phase transitions that alter the physical state of ribonucleoprotein particles contribute to the spacial and temporal organization of the densely packed intracellular environment. This allows cells to organize biologically coupled processes as well as respond to environmental stimuli. RNA plays a key role in phase sep...

journal_title：Journal of molecular biology

authors： Fay MM,Anderson PJ

更新日期：2018-11-02 00:00:00

abstract：:Experimental and theoretical thermodynamic studies of the consequences of 2'-hydroxyl substitution in the RNA UUCG tetraloop show distinct position dependence consistent with the diverse structural contexts of the four-loop ribose hydroxyls in this motif. The results suggest that even for simple substitutions, such as...

journal_title：Journal of molecular biology

authors： Williams DJ,Hall KB

更新日期：2000-03-17 00:00:00

abstract：:The bacteriophage P1 recombinase Cre mediates site-specific recombination between loxP sites. The loxP site consists of two 13 base-pair inverted repeats separated by an eight base-pair spacer region. When DNA containing the loxP site is incubated with Cre, specific cleavages occur within the spacer region, creating a...

journal_title：Journal of molecular biology

authors： Hoess RH,Abremski K

更新日期：1985-02-05 00:00:00

abstract：:The critical role of the ubiquitin-26S proteasome system in regulation of protein homeostasis in eukaryotes is well established. In contrast, the impact of the ubiquitin-independent proteolytic activity of proteasomes is poorly understood. Through biochemical analysis of mammalian lysates, we find that the 20S proteas...

journal_title：Journal of molecular biology

authors： Baugh JM,Viktorova EG,Pilipenko EV

更新日期：2009-02-27 00:00:00

abstract：:DNA gyrase negatively supercoils DNA in a reaction coupled to the binding and hydrolysis of ATP. Limited supercoiling can be achieved in the presence of the non-hydrolysable ATP analogue, 5'-adenylyl beta,gamma-imidodiphosphate (ADPNP). In order to negatively supercoil DNA, gyrase must wrap a length of DNA around itse...

journal_title：Journal of molecular biology

authors： Heddle JG,Mitelheiser S,Maxwell A,Thomson NH

更新日期：2004-03-26 00:00:00

abstract：:Kinetics, thermodynamics and structural aspects of human alpha-thrombin (thrombin) inhibition by newly synthesized low molecular weight derivatives of alpha-azalysine have been investigated. The thrombin catalyzed hydrolysis of N-ethoxycarbonyl-D-Phe-Pro-alpha-azaLys p-nitrophenyl ester (Eoc-D-Phe-Pro-azaLys-ONp) and ...

journal_title：Journal of molecular biology

authors： De Simone G,Balliano G,Milla P,Gallina C,Giordano C,Tarricone C,Rizzi M,Bolognesi M,Ascenzi P

更新日期：1997-06-20 00:00:00

abstract：:We have used nuclear magnetic resonance (NMR) to obtain the structure of an RNA "kissing" hairpin complex formed between the HIV-2 TAR hairpin loop and a hairpin with a complementary loop sequence. Kissing hairpins are important in natural antisense reactions; their complex is a specific target for protein binding. Th...

journal_title：Journal of molecular biology

authors： Chang KY,Tinoco I Jr

更新日期：1997-05-30 00:00:00

abstract：:The 90-kDa heat shock proteins (Hsp90s) assist the maturation of many key regulators of signal transduction pathways and cellular control circuits like protein kinases and transcription factors and chaperone their stability and activity. In this function, Hsp90s cooperate with some 30 cochaperones and they are themsel...

journal_title：Journal of molecular biology

authors： Nguyen MTN,Knieß RA,Daturpalli S,Le Breton L,Ke X,Chen X,Mayer MP

更新日期：2017-03-10 00:00:00

abstract：:Crystals of the mouse major urinary protein (MUP) and rat alpha-2u globulin (AMG) have been grown from solutions of polyethylene glycol 3350 and CdCl2, respectively. The crystals differ both in their morphologies and space groups but have very similar unit cell sizes. AMG crystallized in P2(1) (a = 56.6 A, b = 103.8 A...

journal_title：Journal of molecular biology

authors： Böcskei Z,Findlay JB,North AC,Phillips SE,Somers WS,Wright CE,Lionetti C,Tirindelli R,Cavaggioni A

更新日期：1991-04-20 00:00:00

abstract：:The cylindrical chaperonin GroEL and its cofactor GroES mediate ATP-dependent protein folding in Escherichia coli by transiently encapsulating non-native substrate in a nano-cage formed by the GroEL ring cavity and the lid-shaped GroES. Mechanistic studies of GroEL/ES with heterologous protein substrates suggested tha...

journal_title：Journal of molecular biology

authors： Singh AK,Balchin D,Imamoglu R,Hayer-Hartl M,Hartl FU

更新日期：2020-03-27 00:00:00

abstract：:We present the DNA sequence alterations due to seven lamB missense mutations yielding resistance to phages lambda and K10. They reveal five different amino acid positions in the LamB protein. Three positions (245, 247 and 249) define a new region required for phage adsorption. The two other positions (148 and 152) bel...

journal_title：Journal of molecular biology

authors： Charbit A,Clement JM,Hofnung M

更新日期：1984-05-25 00:00:00

abstract：:Designed retroaldolases have utilized a nucleophilic lysine to promote carbon-carbon bond cleavage of β-hydroxy-ketones via a covalent Schiff base intermediate. Previous computational designs have incorporated a water molecule to facilitate formation and breakdown of the carbinolamine intermediate to give the Schiff b...

journal_title：Journal of molecular biology

authors： Bjelic S,Kipnis Y,Wang L,Pianowski Z,Vorobiev S,Su M,Seetharaman J,Xiao R,Kornhaber G,Hunt JF,Tong L,Hilvert D,Baker D

更新日期：2014-01-09 00:00:00