Location of the SH group of the alkali light chain on the myosin head as revealed by electron microscopy.

Abstract:

:The location of the single cysteinyl residue of the alkali light chain on the myosin head was determined by electron microscopy. The cysteinyl residue of isolated alkali light chain 2 was biotinylated and the light chain was exchanged with that of heavy meromyosin in 4.7 M-NH4Cl. Avidin was attached to the biotin in the heavy meromyosin and the complex was rotary shadowed and observed in the electron microscope. The distance from the head-rod junction to the centre of avidin was 8(+/- 3) nm (mean value +/- standard deviation: n = 105).

journal_name

J Mol Biol

authors

Yamamoto K,Tokunaga M,Sutoh K,Wakabayashi T,Sekine T

doi

10.1016/0022-2836(85)90222-0

subject

Has Abstract

pub_date

1985-05-25 00:00:00

pages

287-90

issue

2

eissn

0022-2836

issn

1089-8638

pii

0022-2836(85)90222-0

journal_volume

183

pub_type

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