Abstract:
:The location of the single cysteinyl residue of the alkali light chain on the myosin head was determined by electron microscopy. The cysteinyl residue of isolated alkali light chain 2 was biotinylated and the light chain was exchanged with that of heavy meromyosin in 4.7 M-NH4Cl. Avidin was attached to the biotin in the heavy meromyosin and the complex was rotary shadowed and observed in the electron microscope. The distance from the head-rod junction to the centre of avidin was 8(+/- 3) nm (mean value +/- standard deviation: n = 105).
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Yamamoto K,Tokunaga M,Sutoh K,Wakabayashi T,Sekine Tdoi
10.1016/0022-2836(85)90222-0subject
Has Abstractpub_date
1985-05-25 00:00:00pages
287-90issue
2eissn
0022-2836issn
1089-8638pii
0022-2836(85)90222-0journal_volume
183pub_type
杂志文章abstract::The projected structure of the vanadate-induced crystalline aggregates of Ca2+-ATPase molecules in isolated sarcoplasmic reticulum membranes has been determined. The molecules form tubular crystals with an oblique surface lattice having cell dimensions a = 65.9 A, b = 114.4 A and gamma = 77.9 degrees. The space group ...
journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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更新日期:1995-02-03 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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更新日期:1994-01-14 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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更新日期:1999-02-05 00:00:00
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journal_title:Journal of molecular biology
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更新日期:2011-11-25 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/0022-2836(87)90038-6
更新日期:1987-08-05 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1006/jmbi.1994.1600
更新日期:1994-09-30 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章,评审
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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abstract::The mitochondrial oxoglutarate carrier (OGC) plays an important role in the malate-aspartate shuttle, the oxoglutarate-isocitrate shuttle and gluconeogenesis. To establish amino acid residues that are important for function, each residue in the transmembrane alpha-helices H1, H3 and H5 was replaced systematically by a...
journal_title:Journal of molecular biology
pub_type: 杂志文章
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abstract::The free form of ribonuclease T1 (RNase T1) has been crystallized at neutral pH, and the three-dimensional structure of the enzyme has been determined at 1.5 A nominal resolution. Restrained least-squares refinement yielded an R value of 14.3% for 12,623 structure amplitudes. The high resolution of the structure analy...
journal_title:Journal of molecular biology
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abstract::The active sites of aspartate transcarbamoylase from Escherichia coli were titrated by measuring the decrease in the enzyme-catalyzed arsenolysis of N-carbamoyl-L-aspartate caused by the addition of the tight-binding inhibitor, N-phosphonacetyl-L-aspartate. Because the enzyme is a poor catalyst for this non-physiologi...
journal_title:Journal of molecular biology
pub_type: 杂志文章
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更新日期:1985-11-05 00:00:00
abstract::Clostridium botulinum C3 exoenzyme inactivates the small GTP-binding protein family Rho by ADP-ribosylating asparagine 41, which depolymerizes the actin cytoskeleton. C3 thus represents a major family of the bacterial toxins that transfer the ADP-ribose moiety of NAD to specific amino acids in acceptor proteins to mod...
journal_title:Journal of molecular biology
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更新日期:2001-01-05 00:00:00
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journal_title:Journal of molecular biology
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abstract::The mechanism of initiation of DNA replication in eukaryotic rolling- circle replicons is still poorly understood in molecular terms. Geminiviruses, a family of plant DNA viruses, which use this strategy during part of their replicative cycle, replicate in the nucleus and are amenable to molecular studies. Except for ...
journal_title:Journal of molecular biology
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更新日期:1999-07-16 00:00:00
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journal_title:Journal of molecular biology
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更新日期:2010-02-12 00:00:00