Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of 3' non-coding regions.

abstract：:In order to investigate the modes of DNA synthesis supported by the 66 and 51 kDa subunits of equine infectious anemia virus reverse transcriptase (EIAV RT), recombinant p66 polypeptides containing a modified ribonuclease H (RNase H) domain were purified and evaluated. Defined heteropolymeric template-primer combinati...

journal_title：Journal of molecular biology

authors： Rausch JW,Arts EJ,Wöhrl BM,Le Grice SF

更新日期：1996-04-05 00:00:00

abstract：:Guanine-uracil (G.U) wobble base-pairs are a detrimental lesion in DNA. Previous investigations have shown that such wobble base-pairs are more prone to base-opening than the normal G.C base-pairs. To investigate the sequence-dependence of base-pair opening we have performed 5ns molecular dynamics simulations on G.U w...

journal_title：Journal of molecular biology

authors： Seibert E,Ross JB,Osman R

更新日期：2003-07-18 00:00:00

abstract：:Chagasin is a protein produced by Trypanosoma cruzi, the parasite that causes Chagas' disease. This small protein belongs to a recently defined family of cysteine protease inhibitors. Although resembling well-known inhibitors like the cystatins in size (110 amino acid residues) and function (they all inhibit papain-li...

journal_title：Journal of molecular biology

authors： Ljunggren A,Redzynia I,Alvarez-Fernandez M,Abrahamson M,Mort JS,Krupa JC,Jaskolski M,Bujacz G

更新日期：2007-08-03 00:00:00

abstract：:Intercellular contacts in multicellular organisms are maintained by membrane receptors called cell adhesion molecules (CAMs), which are expressed on cell surfaces. One interesting feature of CAMs is that almost all of their extracellular regions contain repeating copies of structural domains. It is not clear why so ma...

journal_title：Journal of molecular biology

authors： Chen J,Wu Y

更新日期：2017-04-07 00:00:00

abstract：:We describe light-induced switches for the catalytic activity of the small, RNA-cleaving 8-17 deoxyribozyme (DNAzyme), based on photochemically induced cis-trans isomerization of azobenzene (Az) moieties covalently tethered at various locations within the DNAzyme. Prior studies have shown that trans-azobenzene is able...

journal_title：Journal of molecular biology

authors： Liu Y,Sen D

更新日期：2004-08-20 00:00:00

abstract：:The eukaryotic 26S proteasome is a large multisubunit complex that degrades the majority of proteins in the cell under normal conditions. The 26S proteasome can be divided into two subcomplexes: the 19S regulatory particle and the 20S core particle. Most substrates are first covalently modified by ubiquitin, which the...

journal_title：Journal of molecular biology

authors： Budenholzer L,Cheng CL,Li Y,Hochstrasser M

更新日期：2017-11-10 00:00:00

abstract：:Experimental approaches for the identification of functionally important regions on the surface of a protein involve mutagenesis, in which exposed residues are replaced one after another while the change in binding to other proteins or changes in activity are recorded. However, practical considerations limit the use o...

journal_title：Journal of molecular biology

authors： Armon A,Graur D,Ben-Tal N

更新日期：2001-03-16 00:00:00

abstract：:Calmodulin (CaM) is a calcium binding protein that regulates a wide range of enzymes. Recently the structures of a number of complexes between CaM and synthetic target peptides have been determined. The peptides correspond to the CaM-binding domain of skeletal and smooth muscle myosin light-chain kinase (MLCK) and cal...

journal_title：Journal of molecular biology

authors： Afshar M,Caves LS,Guimard L,Hubbard RE,Calas B,Grassy G,Haiech J

更新日期：1994-12-16 00:00:00

abstract：:Bacteriophage capsid assembly pathways provide excellent model systems to study large-scale conformational changes and other mechanisms that regulate the formation of macromolecular complexes. These capsids are formed from proheads: relatively fragile precursor particles which mature by undergoing extensive remodeling...

journal_title：Journal of molecular biology

authors： Conway JF,Duda RL,Cheng N,Hendrix RW,Steven AC

更新日期：1995-10-13 00:00:00

abstract：:Protein kinase A (PKA) anchoring proteins (AKAPs) tether PKA to various subcellular locations. AKAP121, which tethers PKAII to the outer mitochondrial membrane, includes a K homology (KH) RNA-binding motif. Purified AKAP121 KH domain binds the 3' untranslated regions (3'UTRs) of transcripts encoding the Fo-f subunit o...

journal_title：Journal of molecular biology

authors： Ginsberg MD,Feliciello A,Jones JK,Avvedimento EV,Gottesman ME

更新日期：2003-04-04 00:00:00

abstract：:The three-dimensional structure of the bacteriophage T7 head-to-tail connector has been obtained at 8A resolution using cryo-electron microscopy and single-particle analysis from purified recombinant connectors. The general morphology of the T7 connector is that of a 12-folded toroidal homopolymer with a channel that ...

journal_title：Journal of molecular biology

authors： Agirrezabala X,Martín-Benito J,Valle M,González JM,Valencia A,Valpuesta JM,Carrascosa JL

更新日期：2005-04-15 00:00:00

abstract：:A central challenge in structure-based ligand design is the accurate prediction of binding free energies. Here we apply alchemical free energy calculations in explicit solvent to predict ligand binding in a model cavity in T4 lysozyme. Even in this simple site, there are challenges. We made systematic improvements, be...

journal_title：Journal of molecular biology

authors： Mobley DL,Graves AP,Chodera JD,McReynolds AC,Shoichet BK,Dill KA

更新日期：2007-08-24 00:00:00

abstract：:The rpsO mRNA of E. coli encoding ribosomal protein S15 is destabilized by poly(A) tails posttranscriptionally added by poly(A)polymerase I. We demonstrate here that polyadenylation also contributes to the rapid degradation of mRNA fragments generated by RNase E. It was already known that an RNase E cleavage occurring...

journal_title：Journal of molecular biology

authors： Hajnsdorf E,Régnier P

更新日期：1999-03-05 00:00:00

abstract：:We have developed a simple and efficient method for creation of novel protein functions in an existing protein scaffold. The in vitro coevolution method involves design of a hypothetical pathway for the target function followed by stepwise directed evolution of the corresponding protein along the pathway. As a test ca...

journal_title：Journal of molecular biology

authors： Chen Z,Zhao H

更新日期：2005-05-20 00:00:00

abstract：:We describe experiments that enable us to track the presence and direction of the DNA bend induced by Escherichia coli catabolite activator protein (CAP) through the intermediate stages of transcription initiation at the lac promoter. Transcriptional complexes examined were formed on superhelical templates to enhance ...

journal_title：Journal of molecular biology

authors： Zinkel SS,Crothers DM

更新日期：1991-05-20 00:00:00

abstract：:Escherichia coli thioesterase I (TAP) is a multifunctional enzyme possessing activities of thioesterase, esterase, arylesterase, protease, and lysophospholipase. In particular, TAP has stereoselectivity for amino acid derivative substrates, hence it is useful for the kinetic resolution of racemic mixtures of industria...

journal_title：Journal of molecular biology

authors： Lo YC,Lin SC,Shaw JF,Liaw YC

更新日期：2003-07-11 00:00:00

abstract：:Mannan-binding lectin (MBL) is the archetypical pathogen recognition molecule of the innate immune defense. Upon binding to microorganisms, reactions leading to the destruction of the offender ensue. MBL is an oligomer of structural subunits each composed of three identical polypeptides. We used atomic force microscop...

journal_title：Journal of molecular biology

authors： Jensenius H,Klein DC,van Hecke M,Oosterkamp TH,Schmidt T,Jensenius JC

更新日期：2009-08-07 00:00:00

abstract：:Four new crystal forms of the anti-T lectin from jackfruit (Artocarpus integrifolia) have been prepared and characterized. Three of them, two monoclinic (P21, a = 59.4 A, b = 83.3 A, c = 63.5 A, beta = 107.7 degrees; C2, a = 106.1 A, b = 53.9 A, c = 128.0 A, beta = 95.0 A) and one orthorhombic (C222(1), a = 98.1 A, b ...

journal_title：Journal of molecular biology

authors： Banerjee R,Dhanaraj V,Mahanta SK,Surolia A,Vijayan M

更新日期：1991-10-05 00:00:00

abstract：:To determine the specific interaction sites of topoisomerase II within the DNA region defined by the footprint of the enzyme, we have investigated the cleavage reaction on double-stranded DNA substrates containing nicks and deletions. Topoisomerase II-mediated cleavage of the DNA substrates is suicidal as the enzyme i...

journal_title：Journal of molecular biology

authors： Alsner J,Sorensen HV,Schmidt VK,Sorensen BS,Westergaard O

更新日期：1996-06-14 00:00:00

abstract：:The SfiI endonuclease is a prototype for DNA looping. It binds two copies of its recognition sequence and, if Mg(2+) is present, cuts both concertedly. Looping was examined here on supercoiled and relaxed forms of a 5.5 kb plasmid with three SfiI sites: sites 1 and 2 were separated by 0.4 kb, and sites 2 and 3 by 2.0 ...

journal_title：Journal of molecular biology

authors： Embleton ML,Vologodskii AV,Halford SE

更新日期：2004-05-21 00:00:00

abstract：:The accumulation of senile plaques composed of amyloid β (Aβ) fibrils is a hallmark of Alzheimer's disease, although prefibrillar oligomeric species are believed to be the primary neurotoxic congeners in the pathogenesis of Alzheimer's disease. Uncertainty regarding the mechanistic relationship between Aβ oligomer and...

journal_title：Journal of molecular biology

authors： Doran TM,Anderson EA,Latchney SE,Opanashuk LA,Nilsson BL

更新日期：2012-08-10 00:00:00

abstract：:Many gram-negative bacteria that are important human pathogens possess type III secretion systems as part of their required virulence factor repertoire. During the establishment of infection, these pathogens coordinately assemble greater than 20 different proteins into a macromolecular structure that spans the bacteri...

journal_title：Journal of molecular biology

authors： Zhang L,Wang Y,Picking WL,Picking WD,De Guzman RN

更新日期：2006-06-02 00:00:00

abstract：:Polycystin-1 is a large membrane-associated protein that interacts with polycystin-2 in the primary cilia of renal epithelial cells to form a mechanosensitive ion channel. Bending of the cilia induces calcium flow into the cells, mediated by the polycystin complex. Antibodies to polycystin-1 and polycystin-2 abolish t...

journal_title：Journal of molecular biology

authors： Forman JR,Qamar S,Paci E,Sandford RN,Clarke J

更新日期：2005-06-17 00:00:00

abstract：:The X-ray crystal structure of the sulfide derivative of ferric Lucina pectinata hemoglobin component I (HbI) has been determined at 1.9 A resolution (R-factor 0.186). The heme pocket structural organization of HbI is in keeping with its ligand binding properties. The fast sulfide association rate constant can be rela...

journal_title：Journal of molecular biology

authors： Rizzi M,Wittenberg JB,Coda A,Ascenzi P,Bolognesi M

更新日期：1996-04-26 00:00:00

abstract：:The Integrases of bacteriophages lambda and HK022 promote recombination between DNA molecules that carry attachment sites. The two integrases are about 70% identical in sequence and catalyze nearly identical reactions, but recognize different sets of sites. To identify the amino acids that determine this difference in...

journal_title：Journal of molecular biology

authors： Dorgai L,Yagil E,Weisberg RA

更新日期：1995-09-15 00:00:00

abstract：:The oriC DNA replication origin in bacterial chromosomes, the location of which appears to be physically identified, is genetically regulated by relevant molecular machinery. In contrast, the location of the terminus remains obscure for many bacterial replicons, except for terC, the proposed and well-studied chromosom...

journal_title：Journal of molecular biology

authors： Kono N,Arakawa K,Sato M,Yoshikawa H,Tomita M,Itaya M

更新日期：2014-08-12 00:00:00

abstract：:The histone N tails correspond to conserved amino acid sequences that are peripherally located in the nucleosome and undergo a variety of post-synthetic modifications during cell cycle. These N tails have been recently recognized as directly interacting with transcription-related proteins. We show here, based on circu...

journal_title：Journal of molecular biology

authors： Banères JL,Martin A,Parello J

更新日期：1997-10-31 00:00:00

abstract：:Vinculin is a key regulator of the actin cytoskeleton attachment to the cell membrane at cellular adhesion sites, which is crucial for processes such as cell motility and migration, development, survival, and wound healing. Vinculin loss results in embryonic lethality, cardiovascular diseases, and cancer. Its tail dom...

journal_title：Journal of molecular biology

authors： Lee JH,Rangarajan ES,Vonrhein C,Bricogne G,Izard T

更新日期：2012-10-05 00:00:00

abstract：:Spider dragline silk, one of the strongest polymers in nature, is composed of proteins termed major ampullate spidroin (MaSp) 1 and MaSp2. The N-terminal (NT) domain of MaSp1 produced by the nursery web spider Euprosthenops australis acts as a pH-sensitive relay, mediating spidroin assembly at around pH 6.3. Using ami...

journal_title：Journal of molecular biology

authors： Landreh M,Askarieh G,Nordling K,Hedhammar M,Rising A,Casals C,Astorga-Wells J,Alvelius G,Knight SD,Johansson J,Jörnvall H,Bergman T

更新日期：2010-11-26 00:00:00

abstract：:We previously reported that in Micrococcus luteus, a Gram-positive eubacterium with high genomic G + C content, certain codons ending with A did not appear in coding frames, including termination sites, and tRNAs that translate these codons were not detected. These facts suggest that at least some of them are unassign...

journal_title：Journal of molecular biology

authors： Kano A,Ohama T,Abe R,Osawa S

更新日期：1993-03-05 00:00:00