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FLP recombinase of the 2 microns circle plasmid of Saccharomyces cerevisiae bends its DNA target. Isolation of FLP mutants defective in DNA bending.

Abstract：

:The FLP recombinase is encoded by the yeast plasmid 2 microns circle and catalyses a site-specific recombination reaction that results in inversion of a segment of the 2 micron plasmid. We describe a method for the isolation of inactivating mutations in the FLP gene. The analysis of the recombination and binding activity of defective FLP proteins in vitro resulted in the identification of two classes of mutations: those that completely abolish FLP function by interfering with DNA binding and others that block recombination after the binding step. We have shown that FLP-mediated recombination is accompanied by bending of the DNA target and that mutations in the FLP recombinase that block bending also eliminate recombination.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Schwartz CJ,Sadowski PD

doi

10.1016/0022-2836(89)90310-0

subject

Has Abstract

pub_date

1989-02-20 00:00:00

pages

647-58

issue

4

eissn

0022-2836

issn

1089-8638

pii

0022-2836(89)90310-0

journal_volume

205

pub_type

杂志文章

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