A 50th order reaction predicted and observed for sickle hemoglobin nucleation.


:Sickle hemoglobin polymerization exhibits a striking sensitivity to initial concentration, with characteristic reaction times that exhibit 30th power dependence on concentration. This extraordinary reaction order is encompassed by a novel double nucleation mechanism that predicts 50th power dependence of the homogeneous nucleation rate. Using a technique that allows individual homogeneous nucleation events to be monitored, we have measured a concentration dependence of 47+/-, in excellent agreement with the predictions of the model. Absolute nucleation rates agree with predictions as well.


J Mol Biol


Cao Z,Ferrone FA




Has Abstract


1996-02-23 00:00:00














  • Three-dimensional structure of rotavirus.

    abstract::The three-dimensional structures of double and single-shelled simian rotavirus have been determined to a resolution of 40 A by image processing electron micrographs of unstained, unfixed virus particles embedded in vitreous ice. This study demonstrates that the icosahedral surface lattices in these structures have a t...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Prasad BV,Wang GJ,Clerx JP,Chiu W

    更新日期:1988-01-20 00:00:00

  • Implications of the aquaporin-4 structure on array formation and cell adhesion.

    abstract::Aquaporin-4 (AQP4) is the predominant water channel in the mammalian brain and an important drug target for treatment of cerebral edema, bipolar disorder and mesial temporal lobe epilepsy. We determined the AQP4 structure by electron crystallography of double-layered, two-dimensional (2D) crystals. The structure allow...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Hiroaki Y,Tani K,Kamegawa A,Gyobu N,Nishikawa K,Suzuki H,Walz T,Sasaki S,Mitsuoka K,Kimura K,Mizoguchi A,Fujiyoshi Y

    更新日期:2006-01-27 00:00:00

  • The overall architecture and receptor binding of pneumococcal carbohydrate-antigen-hydrolyzing enzymes.

    abstract::The TIGR4 and SP3-BS71 strains of Streptococcus pneumoniae each produce family 98 glycoside hydrolases, called Sp4GH98 and Sp3GH98, respectively, which have different modular architectures and substrate specificities. Sp4GH98 degrades the Lewis(Y) antigen and possesses three C-terminal family 47 carbohydrate-binding m...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Higgins MA,Ficko-Blean E,Meloncelli PJ,Lowary TL,Boraston AB

    更新日期:2011-09-02 00:00:00

  • Structural Basis for the Interaction between the IUS-SPRY Domain of RanBPM and DDX-4 in Germ Cell Development.

    abstract::RanBPM and RanBP10 are non-canonical members of the Ran binding protein family that lack the Ran binding domain and do not associate with Ran GTPase in vivo. Rather, they have been shown to be scaffolding proteins that are important for a variety of cellular processes, and both of these proteins contain a SPRY domain,...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Hong SK,Kim KH,Song EJ,Kim EE

    更新日期:2016-10-23 00:00:00

  • Determination of the cleavage site of the phage T4 prohead protease in gene product 68. Influence of protein secondary structure on cleavage specificity.

    abstract::The cleavage site of the T4 prohead protease in gene product 68 of bacteriophage T4 has been determined by direct protein sequencing. It is located close to the carboxy-terminal end of a predicted alpha-helix in the sequence Asn-Val-Glu-Ala between the Glu and Ala residues. Secondary structure seems to be more importa...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Keller B,Kellenberger E,Bickle TA,Tsugita A

    更新日期:1985-12-05 00:00:00

  • Accommodation of amino acid insertions in an alpha-helix of T4 lysozyme. Structural and thermodynamic analysis.

    abstract::One to four alanines were inserted by site-directed mutagenesis at three different locations within the alpha-helix comprising residues 39 to 50 in bacteriophage T4 lysozyme. All insertion mutants were correctly folded and catalytically active although the insertions led to a thermal destabilization by 1.1 to 4.2 kcal...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Heinz DW,Baase WA,Zhang XJ,Blaber M,Dahlquist FW,Matthews BW

    更新日期:1994-02-25 00:00:00

  • Crystallization and preliminary X-ray data for the A-isozyme of O-acetylserine sulfhydrylase from Salmonella typhimurium.

    abstract::The A-isozyme of O-acetylserine sulfhydrylase, a pyridoxal phosphate-dependent enzyme isolated from Salmonella typhimurium catalyzes the synthesis of L-cysteine from O-acetyl-L-serine and sulfide. The pyridoxal form of the enzyme has been crystallized in two different forms. One form is in the orthorhombic space group...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Rao GS,Mottonen J,Goldsmith EJ,Cook PF

    更新日期:1993-06-20 00:00:00

  • Effect of Mg(2+) and Na(+) on the nucleic acid chaperone activity of HIV-1 nucleocapsid protein: implications for reverse transcription.

    abstract::The human immunodeficiency virus type 1 (HIV-1) nucleocapsid protein (NC) is an essential protein for retroviral replication. Among its numerous functions, NC is a nucleic acid (NA) chaperone protein that catalyzes NA rearrangements leading to the formation of thermodynamically more stable conformations. In vitro, NC ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Vo MN,Barany G,Rouzina I,Musier-Forsyth K

    更新日期:2009-02-27 00:00:00

  • Vibrio cholerae YaeO is a Structural Homologue of RNA Chaperone Hfq that Inhibits Rho-dependent Transcription Termination by Dissociating its Hexameric State.

    abstract::Rho-dependent transcription termination is a well-conserved process in bacteria. The Psu and YaeO proteins are the two established inhibitors of the ATP-dependent RNA helicase Rho protein of Escherichia coli. Here, we show a detailed sequence and phylogenetic analysis demonstrating that Vibrio cholerae YaeO (VcYaeO) i...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Pal K,Yadav M,Jain S,Ghosh B,Sen R,Sen U

    更新日期:2019-12-06 00:00:00

  • The structure of Arabidopsis thaliana OST1 provides insights into the kinase regulation mechanism in response to osmotic stress.

    abstract::SnRK [SNF1 (sucrose non-fermenting-1)-related protein kinase] 2.6 [open stomata 1 (OST1)] is well characterized at molecular and physiological levels to control stomata closure in response to water-deficit stress. OST1 is a member of a family of 10 protein kinases from Arabidopsis thaliana (SnRK2) that integrates absc...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Yunta C,Martínez-Ripoll M,Zhu JK,Albert A

    更新日期:2011-11-18 00:00:00

  • Base sequence and helix structure variation in B and A DNA.

    abstract::The observed propeller twist in base-pairs of crystalline double-helical DNA oligomers improves the stacking overlap along each individual helix strand. But, as proposed by Calladine, it also leads to clash or steric hindrance between purines at adjacent base-pairs on opposite strands of the helix. This clash can be r...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Dickerson RE

    更新日期:1983-05-25 00:00:00

  • Oxygen transport by fetal bovine hemoglobin.

    abstract::The functional properties of fetal bovine hemoglobin have been studied as a function of temperature, chloride and 2,3-diphosphoglycerate (DPG) concentration. The fetal bovine erythrocyte has six times the concentration of the allosteric modulator DPG compared with the adult cell, and yet the oxygen affinity of the fet...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Clementi ME,Scatena R,Mordente A,Condò SG,Castagnola M,Giardina B

    更新日期:1996-01-12 00:00:00

  • Electron microscopy of decorated crystals for the determination of crystallographic rotation and translation parameters in large protein complexes.

    abstract::The lumazine synthase/riboflavin synthase complex of Bacillus subtilis consists of an icosahedral capsid of 60 beta subunits enclosing a core of 3 alpha subunits. The preparation of reconstituted hollow capsids consisting of 60 beta subunits and their crystallization in a hexagonal (space group P6(3)22) and in a monoc...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Bacher A,Weinkauf S,Bachmann L,Ritsert K,Baumeister W,Huber R,Ladenstein R

    更新日期:1992-06-20 00:00:00

  • Molecular structure of D-hydantoinase from Bacillus sp. AR9: evidence for mercury inhibition.

    abstract::Stereospecific conversion of hydantoins into their carbamoyl acid derivatives could be achieved by using the enzyme hydantoinase. Specific hydantoinases convert either the D-form or the L-form of the hydantoin and the amino acids responsible for stereospecificity have not been identified. Structural studies on hydanto...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Radha Kishan KV,Vohra RM,Ganesan K,Agrawal V,Sharma VM,Sharma R

    更新日期:2005-03-18 00:00:00

  • Celastrol inhibits Tat-mediated human immunodeficiency virus (HIV) transcription and replication.

    abstract::Current drugs used for antiretroviral therapy against human immunodeficiency virus (HIV) have a narrow spectrum of activity and, more often, have associated toxicities and severe side effects in addition to developing resistance. Thus, there is a need to develop new therapeutic strategies against HIV/AIDS to complemen...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Narayan V,Ravindra KC,Chiaro C,Cary D,Aggarwal BB,Henderson AJ,Prabhu KS

    更新日期:2011-07-29 00:00:00

  • Cross-linkage sites in type I collagen fibrils studied by neutron diffraction.

    abstract::Cross-links in tendon collagen are essential for the biomechanical strength of healthy tissue. The nature and position of these cross-links has long been a subject for conjecture. We have approached this problem in a non-destructive manner, by studying neutron diffraction from collagen fibrils that have been specifica...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Wess TJ,Miller A,Bradshaw JP

    更新日期:1990-05-05 00:00:00

  • Direct binding of glyceraldehyde 3-phosphate dehydrogenase to telomeric DNA protects telomeres against chemotherapy-induced rapid degradation.

    abstract::Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that displays several non-glycolytic activities, including the maintenance and/or protection of telomeres. In this study, we determined the molecular mechanism and biological role of the interaction between GAPDH and human telomeric DNA. Using gel...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Demarse NA,Ponnusamy S,Spicer EK,Apohan E,Baatz JE,Ogretmen B,Davies C

    更新日期:2009-12-11 00:00:00

  • Evolution of protein binding modes in homooligomers.

    abstract::The evolution of protein interactions cannot be deciphered without a detailed analysis of interaction interfaces and binding modes. We performed a large-scale study of protein homooligomers in terms of their symmetry, interface sizes, and conservation of binding modes. We also focused specifically on the evolution of ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Dayhoff JE,Shoemaker BA,Bryant SH,Panchenko AR

    更新日期:2010-01-29 00:00:00

  • Quasi-symmetry in the cryo-EM structure of EmrE provides the key to modeling its transmembrane domain.

    abstract::Small multidrug resistance (SMR) transporters contribute to bacterial resistance by coupling the efflux of a wide range of toxic aromatic cations, some of which are commonly used as antibiotics and antiseptics, to proton influx. EmrE is a prototypical small multidrug resistance transporter comprising four transmembran...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Fleishman SJ,Harrington SE,Enosh A,Halperin D,Tate CG,Ben-Tal N

    更新日期:2006-11-17 00:00:00

  • Crystal structure of the dog lipocalin allergen Can f 2: implications for cross-reactivity to the cat allergen Fel d 4.

    abstract::The dog lipocalin allergen Can f 2 is an important cause of allergic sensitization in humans worldwide. Here, the first crystal structure of recombinant rCan f 2 at 1.45 A resolution displays a classical lipocalin fold with a conserved Gly-Xaa-Trp motif, in which Trp19 stabilizes the overall topology of the monomeric ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Madhurantakam C,Nilsson OB,Uchtenhagen H,Konradsen J,Saarne T,Högbom E,Sandalova T,Grönlund H,Achour A

    更新日期:2010-08-06 00:00:00

  • Crystal structure of Hsc20, a J-type Co-chaperone from Escherichia coli.

    abstract::Hsc20 is a 20 kDa J-protein that regulates the ATPase activity and peptide-binding specificity of Hsc66, an hsp70-class molecular chaperone. We report herein the crystal structure of Hsc20 from Escherichia coli determined to a resolution of 1.8 A using a combination of single isomorphous replacement (SIR) and multi-wa...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Cupp-Vickery JR,Vickery LE

    更新日期:2000-12-15 00:00:00

  • Ligand-induced conformational changes via flexible linkers in the amino-terminal region of the inositol 1,4,5-trisphosphate receptor.

    abstract::Cytoplasmic Ca2+ signals are highly regulated by various ion transporters, including the inositol 1,4,5-trisphosphate (IP(3)) receptor (IP(3)R), which functions as a Ca2+ release channel on the endoplasmic reticulum membrane. Crystal structures of the two N-terminal regulatory regions from type 1 IP(3)R have been repo...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Chan J,Whitten AE,Jeffries CM,Bosanac I,Mal TK,Ito J,Porumb H,Michikawa T,Mikoshiba K,Trewhella J,Ikura M

    更新日期:2007-11-09 00:00:00

  • Virtual interaction profiles of proteins.

    abstract::We have developed a new method for the prediction of peptide sequences that bind to a protein, given a three-dimensional structure of the protein in complex with a peptide. By applying a recently developed sequence prediction algorithm and a novel ensemble averaging calculation, we generate a diverse collection of pep...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Wollacott AM,Desjarlais JR

    更新日期:2001-10-19 00:00:00

  • Pactamycin resistance mutations in functional sites of 16 S rRNA.

    abstract::Mutants of an archaeon Halobacterium halobium, resistant to the universal inhibitor of translation, pactamycin, were isolated. Pactamycin resistance correlated with the presence of mutations in the 16 S rRNA gene of H. halobium single rRNA operon. Three types of mutations were found in pactamycin resistant cells, A694...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章,评审


    authors: Mankin AS

    更新日期:1997-11-21 00:00:00

  • Phosphorylation and mutation of phospholamban alter physical interactions with the sarcoplasmic reticulum calcium pump.

    abstract::Phospholamban physically interacts with the sarcoplasmic reticulum calcium pump (SERCA) and regulates contractility of the heart in response to adrenergic stimuli. We studied this interaction using electron microscopy of 2D crystals of SERCA in complex with phospholamban. In earlier studies, phospholamban oligomers we...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Glaves JP,Trieber CA,Ceholski DK,Stokes DL,Young HS

    更新日期:2011-01-21 00:00:00

  • Shape-specific nucleotide binding of single-stranded RNA by the GLD-1 STAR domain.

    abstract::Proteins containing the STAR RNA-binding domain fulfill vital roles in RNA biogenesis, yet a detailed understanding of STAR domain RNA binding specificity is lacking. In Caenorhabditis elegans, the STAR protein GLD-1 directly binds the 28 nucleotide recognition element TGE within the 3' untranslated region of tra-2 mR...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Lehmann-Blount KA,Williamson JR

    更新日期:2005-02-11 00:00:00

  • A critical assessment of putative gatekeeper interactions in the villin headpiece helical subdomain.

    abstract::The helical subdomain of the villin headpiece (HP36) is one of the smallest naturally occurring proteins that folds cooperatively. Its small size, rapid folding, and simple three-helix topology have made it an extraordinary popular model system for computational, theoretical, and experimental studies of protein foldin...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Xiao S,Raleigh DP

    更新日期:2010-08-13 00:00:00

  • DNA sequences required for the initiation of adenovirus type 4 DNA replication in vitro.

    abstract::In-vivo studies have demonstrated that adenovirus type 2 and adenovirus type 4 have different DNA sequence requirements for the initiation of DNA replication. To investigate the basis of these differences an in-vitro system has been developed which will faithfully initiate adenovirus type 4 DNA replication. A plasmid ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Harris MP,Hay RT

    更新日期:1988-05-05 00:00:00

  • Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of 3' non-coding regions.

    abstract::We have isolated and sequenced a cloned complementary DNA insert complementary to the messenger RNA of a cytoplasmic actin expressed in human epidermal cells. This provides the first cytoplasmic actin complementary DNA sequence for a vertebrate organism. The actin amino acid sequence predicted from this complementary ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Hanukoglu I,Tanese N,Fuchs E

    更新日期:1983-02-05 00:00:00

  • Interaction Analyses of the Integrin β2 Cytoplasmic Tail with the F3 FERM Domain of Talin and 14-3-3ζ Reveal a Ternary Complex with Phosphorylated Tail.

    abstract::Integrins, which are heterodimeric (α and β subunits) signal-transducer proteins, are essential for cell adhesion and migration. β cytosolic tails (β-CTs) of integrins interact with a number of cytosolic proteins including talin, Dok1, and 14-3-3ζ. The formation of multiprotein complexes with β-CTs is involved in the ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Chatterjee D,Zhiping LL,Tan SM,Bhattacharjya S

    更新日期:2016-10-09 00:00:00