A 50th order reaction predicted and observed for sickle hemoglobin nucleation.


:Sickle hemoglobin polymerization exhibits a striking sensitivity to initial concentration, with characteristic reaction times that exhibit 30th power dependence on concentration. This extraordinary reaction order is encompassed by a novel double nucleation mechanism that predicts 50th power dependence of the homogeneous nucleation rate. Using a technique that allows individual homogeneous nucleation events to be monitored, we have measured a concentration dependence of 47+/-, in excellent agreement with the predictions of the model. Absolute nucleation rates agree with predictions as well.


J Mol Biol


Cao Z,Ferrone FA




Has Abstract


1996-02-23 00:00:00














  • Refined crystal structure of the catalytic domain of dihydrolipoyl transacetylase (E2p) from Azotobacter vinelandii at 2.6 A resolution.

    abstract::Dihydrolipoyl transacetylase (E2p) is both structurally and functionally the central enzyme of the pyruvate dehydrogenase multienzyme complex. The crystal structure of the catalytic domain, i.e. residues 382 to 637, of Azotobacter vinelandii E2p (E2pCD) was solved by multiple isomorphous replacement and refined by ene...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Mattevi A,Obmolova G,Kalk KH,Westphal AH,de Kok A,Hol WG

    更新日期:1993-04-20 00:00:00

  • The Isoniazid Paradigm of Killing, Resistance, and Persistence in Mycobacterium tuberculosis.

    abstract::Isoniazid (INH) was the first synthesized drug that mediated bactericidal killing of the bacterium Mycobacterium tuberculosis, a major clinical breakthrough. To this day, INH remains a cornerstone of modern tuberculosis (TB) chemotherapy. This review describes the serendipitous discovery of INH, its effectiveness on T...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章,评审


    authors: Vilchèze C,Jacobs WR Jr

    更新日期:2019-08-23 00:00:00

  • The structure of Arabidopsis thaliana OST1 provides insights into the kinase regulation mechanism in response to osmotic stress.

    abstract::SnRK [SNF1 (sucrose non-fermenting-1)-related protein kinase] 2.6 [open stomata 1 (OST1)] is well characterized at molecular and physiological levels to control stomata closure in response to water-deficit stress. OST1 is a member of a family of 10 protein kinases from Arabidopsis thaliana (SnRK2) that integrates absc...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Yunta C,Martínez-Ripoll M,Zhu JK,Albert A

    更新日期:2011-11-18 00:00:00

  • Ancylostoma ceylanicum excretory-secretory protein 2 adopts a netrin-like fold and defines a novel family of nematode proteins.

    abstract::Hookworms are human parasites that have devastating effects on global health, particularly in underdeveloped countries. Ancylostoma ceylanicum infects humans and animals, making it a useful model organism to study disease pathogenesis. A. ceylanicum excretory-secretory protein 2 (AceES-2), a highly immunoreactive mole...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Kucera K,Harrison LM,Cappello M,Modis Y

    更新日期:2011-04-22 00:00:00

  • Structural transitions and thermodynamics of a glycine-dependent riboswitch from Vibrio cholerae.

    abstract::Riboswitches are complex folded RNA domains found in noncoding regions of mRNA that regulate gene expression upon small molecule binding. Recently, Breaker and coworkers reported a tandem aptamer riboswitch (VCI-II) that binds glycine cooperatively. Here, we use hydroxyl radical footprinting and small-angle X-ray scat...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Lipfert J,Das R,Chu VB,Kudaravalli M,Boyd N,Herschlag D,Doniach S

    更新日期:2007-02-02 00:00:00

  • Protein three-dimensional structure determination and sequence-specific assignment of 13C and 15N-separated NOE data. A novel real-space ab initio approach.

    abstract::The sequence-specific assignment of resonances is considered to be a requirement for the determination of the three-dimensional (3D) structure of a protein in solution by nuclear magnetic resonance methods. The main source of structural information is the nuclear Overhauser effect spectroscopy (NOESY) spectrum, which ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Kraulis PJ

    更新日期:1994-11-04 00:00:00

  • A complex RNA-cleaving DNAzyme that can efficiently cleave a pyrimidine-pyrimidine junction.

    abstract::Several RNA-cleaving deoxyribozymes (DNAzymes) have been reported for efficient cleavage of purine-containing junctions, but none is able to efficiently cleave pyrimidine-pyrimidine (Pyr-Pyr) junctions. We hypothesize that a stronger Pyr-Pyr cleavage activity requires larger DNAzymes with complex structures that are d...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Lam JC,Withers JB,Li Y

    更新日期:2010-07-23 00:00:00

  • Flexible stoichiometry and asymmetry of the PIDDosome core complex by heteronuclear NMR spectroscopy and mass spectrometry.

    abstract::Homotypic death domain (DD)-DD interactions are important in the assembly of oligomeric signaling complexes such as the PIDDosome that acts as a platform for activation of caspase-2-dependent apoptotic signaling. The structure of the PIDDosome core complex exhibits an asymmetric three-layered arrangement containing fi...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Nematollahi LA,Garza-Garcia A,Bechara C,Esposito D,Morgner N,Robinson CV,Driscoll PC

    更新日期:2015-02-27 00:00:00

  • Stochastic switching in gene networks can occur by a single-molecule event or many molecular steps.

    abstract::Due to regulatory feedback, biological networks can exist stably in multiple states, leading to heterogeneous phenotypes among genetically identical cells. Random fluctuations in protein numbers, tuned by specific molecular mechanisms, have been hypothesized to drive transitions between these different states. We deve...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Choi PJ,Xie XS,Shakhnovich EI

    更新日期:2010-02-12 00:00:00

  • RNA-DNA hybrid formation at a bacteriophage T4 replication origin.

    abstract::The bacteriophage T4 replication origins ori(uvsY) and ori(34) each contain two distinct components: a T4 middle-mode promoter that is strictly required for replication and a downstream region of about 50 bp that is required for maximal levels of replication. Here, we present evidence that structure of the downstream ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Carles-Kinch K,Kreuzer KN

    更新日期:1997-03-14 00:00:00

  • An in-the-groove view of DNA structures in complexes with proteins.

    abstract::DNA structures bound to transcription factors are studied by using the crystal coordinates of complexes. Structural characteristics which are found at the sites where a protein secondary structure, an alpha-helix or a beta-sheet binds, can be understood in terms of fitting of the concave surface of the DNA major groov...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Suzuki M,Yagi N

    更新日期:1996-02-09 00:00:00

  • A neutral molecule in a cation-binding site: specific binding of a PEG-SH to acetylcholinesterase from Torpedo californica.

    abstract::The crystal structure of acetylcholinesterase from Torpedo californica complexed with the uncharged inhibitor, PEG-SH-350 (containing mainly heptameric polyethylene glycol with a terminal thiol group) is determined at 2.3 A resolution. This is an untypical acetylcholinesterase inhibitor, since it lacks the cationic mo...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Koellner G,Steiner T,Millard CB,Silman I,Sussman JL

    更新日期:2002-07-19 00:00:00

  • Translation initiation factor 3 regulates switching between different modes of ribosomal subunit joining.

    abstract::Ribosomal subunit joining is a key checkpoint in the bacterial translation initiation pathway during which initiation factors (IFs) regulate association of the 30S initiation complex (IC) with the 50S subunit to control formation of a 70S IC that can enter into the elongation stage of protein synthesis. The GTP-bound ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: MacDougall DD,Gonzalez RL Jr

    更新日期:2015-05-08 00:00:00

  • Structure-function defects of the TWINKLE linker region in progressive external ophthalmoplegia.

    abstract::TWINKLE is the helicase at the mitochondrial DNA (mtDNA) replication fork in mammalian cells. Mutations in the PEO1 gene, which encodes TWINKLE, cause autosomal dominant progressive external ophthalmoplegia (AdPEO), a disorder associated with deletions in mtDNA. Here, we characterized seven different AdPEO-causing mut...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Korhonen JA,Pande V,Holmlund T,Farge G,Pham XH,Nilsson L,Falkenberg M

    更新日期:2008-03-28 00:00:00

  • Sequence-specific DNA binding of the phage Mu C protein: footprinting analysis reveals altered DNA conformation upon protein binding.

    abstract::The mom gene of bacteriophage Mu, which codes for a DNA modification function, is regulated in a complex manner at both transcriptional and translational levels. The phage-encoded C protein functions as an activator of mom transcription. The mom promoter has features of an activator-dependent weak promoter, and the C ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Ramesh V,Nagaraja V

    更新日期:1996-07-05 00:00:00

  • phi29 DNA polymerase active site: role of residue Val250 as metal-dNTP complex ligand and in protein-primed initiation.

    abstract::DNA polymerases require two acidic residues to coordinate metal ions A and B at their polymerisation active site during catalysis of nucleotide incorporation. Crystallographic resolution of varphi29 DNA polymerase ternary complex showed that metal B coordination also depends on the carbonyl group of Val250 that belong...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Pérez-Arnaiz P,Lázaro JM,Salas M,de Vega M

    更新日期:2010-01-15 00:00:00

  • How Widespread is Metabolite Sensing by Ribosome-Arresting Nascent Peptides?

    abstract::In order to colonize a niche and compete for scarce resources, microorganisms have evolved means to adjust the expression levels of their biosynthetic enzymes in response to the changing levels of metabolites available to them. To do so, they often rely on transcription factors or structured RNAs that directly sense t...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章,评审


    authors: Seip B,Innis CA

    更新日期:2016-05-22 00:00:00

  • Crystal structure of Hsc20, a J-type Co-chaperone from Escherichia coli.

    abstract::Hsc20 is a 20 kDa J-protein that regulates the ATPase activity and peptide-binding specificity of Hsc66, an hsp70-class molecular chaperone. We report herein the crystal structure of Hsc20 from Escherichia coli determined to a resolution of 1.8 A using a combination of single isomorphous replacement (SIR) and multi-wa...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Cupp-Vickery JR,Vickery LE

    更新日期:2000-12-15 00:00:00

  • The crystal structure of a sulfurtransferase from Azotobacter vinelandii highlights the evolutionary relationship between the rhodanese and phosphatase enzyme families.

    abstract::Rhodanese is an ubiquitous enzyme that in vitro catalyses the transfer of a sulfur atom from suitable donors to nucleophilic acceptors by way of a double displacement mechanism. During the catalytic process the enzyme cycles between a sulfur-free and a persulfide-containing form, via formation of a persulfide linkage ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Bordo D,Deriu D,Colnaghi R,Carpen A,Pagani S,Bolognesi M

    更新日期:2000-05-12 00:00:00

  • First glimpses at structure-function relationships of the nucleocapsid protein of retroviruses.

    abstract::Retroviruses are a family of widespread small animal viruses about 110 nm in diameter, composed of an inner core surrounded by an outer envelope formed of a lipid bilayer of cellular origin in which are anchored viral glycoproteins. The inner core is formed by an outer shell of capsid protein molecules (CA protein) su...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章,评审


    authors: Darlix JL,Lapadat-Tapolsky M,de Rocquigny H,Roques BP

    更新日期:1995-12-08 00:00:00

  • Recognition and manipulation of branched DNA structure by junction-resolving enzymes.

    abstract::The junction-resolving enzymes are a class of nucleases that introduce paired cleavages into four-way DNA junctions. They are important in DNA recombination and repair, and are found throughout nature, from eubacteria and their bacteriophages through to higher eukaryotes and their viruses. These enzymes exhibit struct...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章,评审


    authors: White MF,Giraud-Panis MJ,Pöhler JR,Lilley DM

    更新日期:1997-06-27 00:00:00

  • Kinetic buffering of cross talk between bacterial two-component sensors.

    abstract::Two-component systems are a class of sensors that enable bacteria to respond to environmental and cell-state signals. The canonical system consists of a membrane-bound sensor histidine kinase that autophosphorylates in response to a signal and transfers the phosphate to an intracellular response regulator. Bacteria ty...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Groban ES,Clarke EJ,Salis HM,Miller SM,Voigt CA

    更新日期:2009-07-17 00:00:00

  • Titration calculations of foot-and-mouth disease virus capsids and their stabilities as a function of pH.

    abstract::Foot-and-mouth disease virus (FMDV), a non-enveloped picornavirus, is sensitive to acidic conditions. At pH values below 7 the icosahedral virus capsid, formed from 60 copies of a protomer containing four polypeptides (VP1 to 4), dissociates into 12 pentamers, releasing the viral RNA. Evidence suggests that this acid ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: van Vlijmen HW,Curry S,Schaefer M,Karplus M

    更新日期:1998-01-16 00:00:00

  • Reconstituted and native iron-cores of bacterioferritin and ferritin.

    abstract::The structural and magnetic properties of the iron-cores of reconstituted horse spleen ferritin and Azotobacter vinelandii bacterioferritin have been investigated by high-resolution transmission electron microscopy, electron diffraction and Mossbauer spectroscopy. The structural properties of native horse spleen ferri...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Mann S,Williams JM,Treffry A,Harrison PM

    更新日期:1987-12-05 00:00:00

  • Supramolecular Assembly of Human Pulmonary Surfactant Protein SP-D.

    abstract::Pulmonary surfactant protein D (SP-D) is a glycoprotein from the collectin family that is a component of the lung surfactant system. It exhibits host defense and immune regulatory functions in addition to contributing to the homeostasis of the surfactant pool within the alveolar airspaces. It is known that the SP-D mo...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Arroyo R,Martín-González A,Echaide M,Jain A,Brondyk WH,Rosenbaum J,Moreno-Herrero F,Pérez-Gil J

    更新日期:2018-05-11 00:00:00

  • Recapitulation of protein family divergence using flexible backbone protein design.

    abstract::We use flexible backbone protein design to explore the sequence and structure neighborhoods of naturally occurring proteins. The method samples sequence and structure space in the vicinity of a known sequence and structure by alternately optimizing the sequence for a fixed protein backbone using rotamer based sequence...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Saunders CT,Baker D

    更新日期:2005-02-18 00:00:00

  • Diversity of molecular transformations involved in the formation of spider silks.

    abstract::Spiders that spin orb webs secrete seven types of silk. Although the spinning process of the dragline thread is beginning to be understood, the molecular events that occur in spiders' opisthosomal glands, which produce the other fibers, are unknown due to a lack of data regarding their initial and final structures. Ta...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Lefèvre T,Boudreault S,Cloutier C,Pézolet M

    更新日期:2011-01-07 00:00:00

  • Fourteen protomers compose the oligomer III of the proton-rotor in spinach chloroplast ATP synthase.

    abstract::Three fundamentally different chloroplast ATP synthase samples of increasing complexity were visualized by atomic force microscopy. The samples are distinguishable in respect to the isolation technique, the detergent employed, and the final subunit composition. The homo-oligomer III was isolated following SDS treatmen...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Seelert H,Dencher NA,Müller DJ

    更新日期:2003-10-17 00:00:00

  • Zinc finger mutations that alter domain interactions in the glucocorticoid receptor.

    abstract::The DNA binding domain of steroid receptors coincides with the cysteine-rich region encompassing the two conserved zinc fingers. In the case of the glucocorticoid receptor (GR), a weak transactivation function has been described to be adjacent or partly overlapping to the DNA binding domain, whereas stronger trans-act...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Zandi E,Galli I,Döbbeling U,Rusconi S

    更新日期:1993-03-05 00:00:00

  • Sampling bottlenecks in de novo protein structure prediction.

    abstract::The primary obstacle to de novo protein structure prediction is conformational sampling: the native state generally has lower free energy than nonnative structures but is exceedingly difficult to locate. Structure predictions with atomic level accuracy have been made for small proteins using the Rosetta structure pred...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Kim DE,Blum B,Bradley P,Baker D

    更新日期:2009-10-16 00:00:00