A 50th order reaction predicted and observed for sickle hemoglobin nucleation.

abstract：:Switching between the active (ATP and DNA bound) and inactive conformations of the homologous recombination RecA protein is regulated by ATP hydrolysis. First, we use the homologous pairing domain of RecA derived from its mobile loop L2 to show that the interaction of this random coil peptide with the gamma-phosphate ...

journal_title：Journal of molecular biology

authors： Voloshin ON,Wang L,Camerini-Otero RD

更新日期：2000-11-10 00:00:00

abstract：:Archaebacterial respiratory chains are poorly understood at the molecular level. We have cloned and sequenced a cluster of five new genes from the archaebacterium Sulfolobus acidocaldarius, four of them coding for redox proteins: a Rieske iron-sulphur protein, a cytochrome b, a subunit II of cytochrome oxidase and a b...

journal_title：Journal of molecular biology

authors： Castresana J,Lübben M,Saraste M

更新日期：1995-07-07 00:00:00

abstract：:The side-chains of phenylalanine and tyrosine residues in proteins are frequently found to be involved in pairwise interactions. These occur both within repeating elements of secondary structure and in tertiary and quaternary interactions. It has been suggested that they are important in protein folding and stability,...

journal_title：Journal of molecular biology

authors： Serrano L,Bycroft M,Fersht AR

更新日期：1991-03-20 00:00:00

abstract：:The nucleotide sequences of nine genes corresponding to tRNA(Ser)4 or tRNA(Ser)7 of Drosophila melanogaster were determined. Eight of the genes compose the major tRNA(Ser)4,7 cluster at 12DE on the X chromosome, while the other is from 23E on the left arm of chromosome 2. Among the eight X-linked genes, five different...

journal_title：Journal of molecular biology

authors： Cribbs DL,Leung J,Newton CH,Hayashi S,Miller RC Jr,Tener GM

更新日期：1987-10-05 00:00:00

abstract：:The protection of telomeres 1 (Pot1) proteins specifically recognize the single-stranded 3' end of the telomere, an activity essential for sustained cellular viability and proliferation. The current model for the telomeric single-stranded DNA (ssDNA) binding activity of Schizosaccharomyces pombe Pot1 is based on a 20 ...

journal_title：Journal of molecular biology

authors： Croy JE,Podell ER,Wuttke DS

更新日期：2006-08-04 00:00:00

abstract：:Promiscuous mutant EcoRI endonucleases produce lethal to sublethal effects because they cleave Escherichia coli DNA despite the presence of the EcoRI methylase. Three promiscuous mutant forms, Ala138Thr, Glu192Lys and His114Tyr, have been characterized with respect to their binding affinities and first-order cleavage ...

journal_title：Journal of molecular biology

authors： Sapienza PJ,Dela Torre CA,McCoy WH 4th,Jana SV,Jen-Jacobson L

更新日期：2005-04-29 00:00:00

abstract：:Telomerase is a ribonucleoprotein complex that replicates the 3' ends of linear chromosomes by successive additions of telomere repeat DNA. The telomerase holoenzyme contains two essential components for catalysis, a telomerase reverse transcriptase (TERT) and telomerase RNA (TER). The TER includes a template for telo...

journal_title：Journal of molecular biology

authors： Kim NK,Zhang Q,Zhou J,Theimer CA,Peterson RD,Feigon J

更新日期：2008-12-31 00:00:00

abstract：:During homologous recombination, genetic information is physically exchanged between parental DNAs via crossing single strands of the same polarity within a four-way DNA junction called a Holliday structure. This process is terminated by the endonucleolytic activity of resolvases, which convert the four-way DNA back t...

journal_title：Journal of molecular biology

authors： Birkenbihl RP,Kemper B

更新日期：2002-08-02 00:00:00

abstract：:Sho1p, an integral membrane protein, plays a vital role in the high-osmolarity glycerol (HOG) mitogen-activated protein kinase pathway in the yeast Saccharomyces cerevisiae. Activated under conditions of high osmotic stress, it interacts with other HOG pathway proteins to mediate cell signaling events, ensuring that y...

journal_title：Journal of molecular biology

authors： Lam MH,Snider J,Rehal M,Wong V,Aboualizadeh F,Drecun L,Wong O,Jubran B,Li M,Ali M,Jessulat M,Deineko V,Miller R,Lee Me,Park HO,Davidson A,Babu M,Stagljar I

更新日期：2015-06-05 00:00:00

abstract：:Dihydropyrimidinases (hydantoinases) catalyse the reversible hydrolytic ring-opening of cyclic diamides such as dihydropyrimidines in the catabolism of pyrimidines. In biotechnology, these enzymes find application in the enantiospecific production of amino acids from racemic hydantoins. The crystal structure of a D-en...

journal_title：Journal of molecular biology

authors： Abendroth J,Niefind K,Schomburg D

更新日期：2002-06-28 00:00:00

abstract：:Atomic contact potentials are derived by statistical analysis of atomic surface contact areas versus atom type in a database of non-homologous protein structures. The atomic environment is characterized by the surface area accessible to solvent and the surface of contacts with polar and non-polar atoms. Four types of ...

journal_title：Journal of molecular biology

authors： Delarue M,Koehl P

更新日期：1995-06-09 00:00:00

abstract：:Tumor suppressor p53 plays a central role in tumor suppression. p53 is the most frequently mutated gene in human cancer, and over half of human cancers contain p53 mutations. Majority of p53 mutations in cancer are missense mutations, leading to the expression of full-length mutant p53 (mutp53) protein. While the crit...

journal_title：Journal of molecular biology

authors： Yue X,Zhao Y,Xu Y,Zheng M,Feng Z,Hu W

更新日期：2017-06-02 00:00:00

abstract：:The steps of UUC recognition by tRNAPhe were analysed by temperature-jump measurements. At ion concentrations close to physiological conditions we found three relaxation processes, which we assigned to (1) formation of codon-anticodon complexes, (2) a conformational change of the anticodon loop coupled with Mg2+ bindi...

journal_title：Journal of molecular biology

authors： Labuda D,Striker G,Porschke D

更新日期：1984-04-25 00:00:00

abstract：:Kinetic models were developed to describe the influence of prolyl peptide bond isomerization on the kinetics of reversible protein folding for cases in which structural intermediates do not occur. In the simulations, the number of prolyl residues and the relative rates of folding and isomerization were varied. The exp...

journal_title：Journal of molecular biology

authors： Kiefhaber T,Kohler HH,Schmid FX

更新日期：1992-03-05 00:00:00

abstract：:The structure of a class II fructose-1,6-bisphosphate aldolase in complex with the substrate analogue and inhibitor phosphoglycolohydroxamate (PGH) has been determined using X-ray diffraction terms to a resolution of 2.0 A (1 A=0.1 nm). The crystals are trigonal, space group P3121 with a=b=78.24 A, c=289.69 A. The asy...

journal_title：Journal of molecular biology

authors： Hall DR,Leonard GA,Reed CD,Watt CI,Berry A,Hunter WN

更新日期：1999-03-26 00:00:00

abstract：:New methods for collecting cross-relaxation data from proteins and nucleic acids make it possible to improve the accuracy and precision of interproton distance measurements used as input for NMR solution structure determinations. It thus is of interest to determine whether such experimentally achievable improvements i...

journal_title：Journal of molecular biology

authors： Hoogstraten CG,Markley JL

更新日期：1996-05-03 00:00:00

abstract：:Phosphofructokinase-1 and -2 (Pfk-1 and Pfk-2, respectively) from Escherichia coli belong to different homologous superfamilies. However, in spite of the lack of a common ancestor, they share the ability to catalyze the same reaction and are inhibited by the substrate MgATP. Pfk-2, an ATP-dependent 6-phosphofructokina...

journal_title：Journal of molecular biology

authors： Cabrera R,Ambrosio AL,Garratt RC,Guixé V,Babul J

更新日期：2008-11-14 00:00:00

abstract：:The pfkB gene of Escherichia coli is known to specify a minor phosphofructokinase, Pfk-2, in the wild-type strain; the pfkB1 mutation causes a 25-fold increase in the amount of Pfk-2 so that it adequately substitutes for mutational loss of the major phosphofructokinase, Pfk-1 (specified by pfkA); and another closely l...

journal_title：Journal of molecular biology

authors： Daldal F

更新日期：1983-08-05 00:00:00

abstract：:Active variant surface glycoprotein (VSG) gene chromatin is preferentially digested by the restriction enzyme HinfI in nuclei of bloodstream variants of Trypanosoma brucei. HinfI sensitivity of VSG gene chromatin is not observed in nuclei of relapse variants in which the VSG gene has been inactivated in situ. Active V...

journal_title：Journal of molecular biology

authors： Greaves DR,Borst P

更新日期：1987-10-05 00:00:00

abstract：:The structure of subfragment 1 (S1) bound to F-actin has been compared to the structure of free S1 using neutron scattering. The F-actin was rendered "invisible" to neutrons by selective deuteration and solvent contrast matching. Highly deuterated actin was purified from the slime mold Dictyostelium discoideum, which ...

journal_title：Journal of molecular biology

authors： Curmi PM,Stone DB,Schneider DK,Spudich JA,Mendelson RA

更新日期：1988-10-05 00:00:00

abstract：:The recent growth in protein databases has revealed the functional diversity of many protein superfamilies. We have assessed the functional variation of homologous enzyme superfamilies containing two or more enzymes, as defined by the CATH protein structure classification, by way of the Enzyme Commission (EC) scheme. ...

journal_title：Journal of molecular biology

authors： Todd AE,Orengo CA,Thornton JM

更新日期：2001-04-06 00:00:00

abstract：:Cytochrome P450cam catalyzes the hydroxylation of camphor in a complex process involving two electron transfers (ETs) from the iron-sulfur protein putidaredoxin. The enzymatic control of the successive steps of catalysis is critical for a highly efficient reaction. The injection of the successive electrons is part of ...

journal_title：Journal of molecular biology

authors： Hiruma Y,Hass MA,Kikui Y,Liu WM,Ölmez B,Skinner SP,Blok A,Kloosterman A,Koteishi H,Löhr F,Schwalbe H,Nojiri M,Ubbink M

更新日期：2013-11-15 00:00:00

abstract：:Alpha-tocopherol transfer protein (alpha-TTP) is a liver protein responsible for the selective retention of alpha-tocopherol from dietary vitamin E, which is a mixture of alpha, beta, gamma, and delta-tocopherols and the corresponding tocotrienols. The alpha-TTP-mediated transfer of alpha-tocopherol into nascent VLDL ...

journal_title：Journal of molecular biology

authors： Meier R,Tomizaki T,Schulze-Briese C,Baumann U,Stocker A

更新日期：2003-08-15 00:00:00

abstract：:Three fundamentally different chloroplast ATP synthase samples of increasing complexity were visualized by atomic force microscopy. The samples are distinguishable in respect to the isolation technique, the detergent employed, and the final subunit composition. The homo-oligomer III was isolated following SDS treatmen...

journal_title：Journal of molecular biology

authors： Seelert H,Dencher NA,Müller DJ

更新日期：2003-10-17 00:00:00

abstract：:Crystals of NADPH:FMN oxidoreductase from Vibrio harveyi have been obtained and characterized by X-ray diffraction. This enzyme plays a role in the generation of light in luminescent bacteria by providing reduced FMN to luciferase. Large, high quality crystals were grown using polyethylene glycol 6000 at pH 7.0. They ...

journal_title：Journal of molecular biology

authors： Tanner J,Lei B,Liu M,Tu SC,Krause KL

更新日期：1994-08-12 00:00:00

abstract：:The periplasmic ribose and glucose-galactose receptors (binding proteins) of Gram-negative bacteria compete for a common inner membrane receptor in bacterial chemotaxis, as well as being the essential primary receptors for their respective membrane transport systems. The high-resolution structures of the periplasmic r...

journal_title：Journal of molecular biology

authors： Mowbray SL

更新日期：1992-09-20 00:00:00

abstract：:The Hippo pathway plays a crucial role in growth control, proliferation and tumor suppression. Activity of the signaling pathway is associated with cell density sensing and tissue organization. Furthermore, the Hippo pathway helps to coordinate cellular processes through crosstalk with growth-factor-mediated signaling...

journal_title：Journal of molecular biology

authors： Grannas K,Arngården L,Lönn P,Mazurkiewicz M,Blokzijl A,Zieba A,Söderberg O

更新日期：2015-10-23 00:00:00

abstract：:Crystal structures of the enzyme creatine amidinohydrolase (creatinase, EC 3.5.3.3) with two different inhibitors, the reaction product sarcosine and the substrate creatine, bound have been analyzed by X-ray diffraction methods. With the inhibitor carbamoyl sarcosine, two different crystal forms at different pH values...

journal_title：Journal of molecular biology

authors： Coll M,Knof SH,Ohga Y,Messerschmidt A,Huber R,Moellering H,Rüssmann L,Schumacher G

更新日期：1990-07-20 00:00:00

abstract：:Activation of family A G-protein-coupled receptors involves a rearrangement of a conserved interhelical cytoplasmic hydrogen bond network between the E(D)RY motif on transmembrane helix 3 (H3) and residues on H6, which is commonly termed the cytoplasmic "ionic lock." Glu134(3.49) of the E(D)RY motif also forms an intr...

journal_title：Journal of molecular biology

authors： Vogel R,Mahalingam M,Lüdeke S,Huber T,Siebert F,Sakmar TP

更新日期：2008-07-18 00:00:00

abstract：:Domains are considered as the basic units of protein folding, evolution, and function. Decomposing each protein into modular domains is thus a basic prerequisite for accurate functional classification of biological molecules. Here, we present ADDA, an automatic algorithm for domain decomposition and clustering of all ...

journal_title：Journal of molecular biology

authors： Heger A,Holm L

更新日期：2003-05-02 00:00:00