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Trypanosoma brucei variant-specific glycoprotein gene chromatin is sensitive to single-strand-specific endonuclease digestion.

Abstract：

:Active variant surface glycoprotein (VSG) gene chromatin is preferentially digested by the restriction enzyme HinfI in nuclei of bloodstream variants of Trypanosoma brucei. HinfI sensitivity of VSG gene chromatin is not observed in nuclei of relapse variants in which the VSG gene has been inactivated in situ. Active VSG gene chromatin is preferentially degraded by the single-strand-specific endonucleases S1 and Bal31. This sensitivity is not the result of pre-existing single-strand breaks or a detectably altered nucleosomal organization. Trypanosome nuclei in which the run-on transcription of VSG genes has been specifically shut down have been used to show that Hinfl and Bal31 sensitivity is not dependent upon continued transcription of the VSG gene. The presence of single-stranded DNA regions within VSG gene chromatin is consistent with a model in which VSG genes are activated by increased torsional stress.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Greaves DR,Borst P

doi

10.1016/0022-2836(87)90559-6

subject

Has Abstract

pub_date

1987-10-05 00:00:00

pages

471-83

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(87)90559-6

journal_volume

197

pub_type

杂志文章

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