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The mathematics of SELEX against complex targets.

Abstract：

:We have developed a computer model for the simulation of simultaneous SELEX against multiple targets. The model assumes equilibrium behavior for the formation of binary ligand:target complexes, and that there is no ligand:ligand or target:target interaction. Target concentrations, ligand concentrations, and affinity distributions of the initial ligand pool for each individual target may be set by the user. We have used this program to gain an understanding of how the presence of multiple targets affects the selection process. In most cases, we find that SELEX is capable of generating different ligands for the different targets in a heterogeneous mixture, regardless of large variations in target concentrations and ligand:target affinities. A low relative partitioning efficiency (the efficiency with which ligands complexed with a target are separated from free ligands) for a target in a mixture gives a greatly reduced rate of selection of high-affinity ligands to that target. The ratio of each high-affinity ligand to its individual target within a pool of ligands selected for binding against a mixture of targets is approximately proportional to the concentration of the target multiplied by the ligand:target partitioning efficiency.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Vant-Hull B,Payano-Baez A,Davis RH,Gold L

doi

10.1006/jmbi.1998.1727

subject

Has Abstract

pub_date

1998-05-08 00:00:00

pages

579-97

issue

3

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(98)91727-2

journal_volume

278

pub_type

杂志文章

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