Crystallization and preliminary X-ray analysis of human alpha-galactosidase A complex.

abstract：:Galanthus nivalis agglutinin (GNA, a 50 kDa tetramer) is a mannose-specific lectin of the Amaryllidaceae family of bulb lectins. Crystal structures of GNA complexed with methyl-alpha-D-mannose (MeMan) and mannose-alpha 1,3-D-mannose-alpha-OMe (MeMan-2) have been determined and analyzed in terms of internal structural ...

journal_title：Journal of molecular biology

authors： Hester G,Wright CS

更新日期：1996-10-04 00:00:00

abstract：:In all living cells, regulated passage across membranes of specific proteins occurs through a universally conserved secretory channel. In bacteria and chloroplasts, the energy for the mechanical work of moving polypeptides through that channel is provided by SecA, a regulated ATPase. Here, we use site-directed spin la...

journal_title：Journal of molecular biology

authors： Cooper DB,Smith VF,Crane JM,Roth HC,Lilly AA,Randall LL

更新日期：2008-09-26 00:00:00

abstract：:Here, we present the basic structural properties and the thermodynamic description of a previously unknown alkali molten globule state of horse "ferricytochrome c". Both sodium and guanidinium cations stabilize the alkali-denatured state at pH 13, presumably by a charge screening mechanism. The Na(+)-stabilized confor...

journal_title：Journal of molecular biology

authors： Kumar R,Prabhu NP,Rao DK,Bhuyan AK

更新日期：2006-12-01 00:00:00

abstract：:Recombination between RNAs associated with turnip crinkle virus is thought to occur during plus-strand synthesis at motifs resembling the 5'-ends of genomic, subgenomic and satellite RNAs. Common structural regions encompassing the motifs have been found for major crossover sites on two different minus-strand template...

journal_title：Journal of molecular biology

authors： Carpenter CD,Oh JW,Zhang C,Simon AE

更新日期：1995-02-03 00:00:00

abstract：:Two major methods are currently being used to characterize transient intermediates during protein folding at the level of individual residues. Nuclear magnetic resonance (n.m.r.) measurements on the protection of peptide NH hydrogens against exchange with solvent during refolding can provide information about secondar...

journal_title：Journal of molecular biology

authors： Matouschek A,Serrano L,Meiering EM,Bycroft M,Fersht AR

更新日期：1992-04-05 00:00:00

abstract：:The structure of the integral membrane protein OmpX from Escherichia coli reconstituted in 60 kDa DHPC micelles (OmpX/DHPC) was calculated from 526 NOE upper limit distance constraints. The structure determination was based on complete sequence-specific assignments for the amide protons and the Val, Leu, and Ile(delta...

journal_title：Journal of molecular biology

authors： Fernández C,Hilty C,Wider G,Güntert P,Wüthrich K

更新日期：2004-03-05 00:00:00

abstract：:We find that tRNA gene transcription is controlled by a region much larger than the previously described internal segments known as A and B boxes. In a Bombyx mori silkworm tRNA2Ala gene, transcriptionally important sequences extend at least from -13 to +146, and thus include sequences both upstream and downstream fro...

journal_title：Journal of molecular biology

authors： Wilson ET,Larson D,Young LS,Sprague KU

更新日期：1985-05-25 00:00:00

abstract：:Bloom syndrome protein (BLM) is one of five human RecQ helicases that participate in DNA metabolism. RecQ C-terminal (RQC) domain is the main DNA binding module of BLM and specifically recognizes G-quadruplex (G4) DNA structures. Because G4 processing by BLM is essential for regulating replication and transcription, b...

journal_title：Journal of molecular biology

authors： Lee S,Lee AR,Ryu KS,Lee JH,Park CJ

更新日期：2019-02-15 00:00:00

abstract：:It has been suggested that modified nucleosides of the xm5(s2)U(m)34-type restrict the wobble capacity of the base, and that their function is to prevent misreading in the third position of the codon in mixed codon family boxes that encode two different amino acids. In this study in Escherichia coli, the misreading in...

journal_title：Journal of molecular biology

authors： Hagervall TG,Pomerantz SC,McCloskey JA

更新日期：1998-11-20 00:00:00

abstract：:The structure of a metastable folding intermediate of human insulin-like growth factor I (IGF-I) and an engineered model are investigated by circular dichroism and two-dimensional 1H NMR spectroscopy. The intermediate, which contains two of three native disulfide bonds, was trapped by acid quenching and isolated by re...

journal_title：Journal of molecular biology

authors： Hua QX,Narhi L,Jia W,Arakawa T,Rosenfeld R,Hawkins N,Miller JA,Weiss MA

更新日期：1996-06-07 00:00:00

abstract：:When influenza A virus infects host cells, its integral matrix protein M2 forms a proton-selective channel in the viral envelope. Although X-ray crystallography and NMR studies using fragment peptides have suggested that M2 stably forms a tetrameric channel irrespective of pH, the oligomeric states of the full-length ...

journal_title：Journal of molecular biology

authors： Kawano K,Yano Y,Matsuzaki K

更新日期：2014-07-15 00:00:00

abstract：:The telomeric d(GGGGTT).d(AACCCC) repeat tracts (G4T2 repeats) in Tetrahymena thermophila macronuclei were shown previously to be packaged in a non-nucleosomal DNA-protein complex. Here, we demonstrate that these telomeric repeats, together with a short region of the immediately adjacent non-telomeric sequence, exist ...

journal_title：Journal of molecular biology

authors： Cohen P,Blackburn EH

更新日期：1998-07-17 00:00:00

abstract：:A multiple alignment of five (beta/alpha)8-barrel enzymes has been derived from their structure. The eight beta-strands and eight alpha-helices of the (beta/alpha)8-barrel are correctly aligned and the equivalenced residues in these regions fulfil similar structural roles. Each beta-strand has a central core of usuall...

journal_title：Journal of molecular biology

authors： Pickett SD,Saqi MA,Sternberg MJ

更新日期：1992-11-05 00:00:00

abstract：:S1 nuclease mapping experiments performed with RNA extracted from cell lines that were unable to metabolize L-rhamnose demonstrated that L-rhamnose and not a metabolite was the inducer of the L-rhamnose operons of Escherichia coli. In vitro transcription studies showed that purified RhaR activates transcription from t...

journal_title：Journal of molecular biology

authors： Tobin JF,Schleif RF

更新日期：1990-01-05 00:00:00

abstract：:Determining the critical structural features a ligand must possess in order to bind to its receptor is of key importance to the understanding of vital biological processes and to the rational design of small molecule therapeutics to modulate receptor function. We have developed a general strategy for determining such ...

journal_title：Journal of molecular biology

authors： Booth V,Slupsky CM,Clark-Lewis I,Sykes BD

更新日期：2003-03-21 00:00:00

abstract：:Based on structural analyses and on the behavior of mutants, we suggest that the polymerase domain of HIV-1 reverse transcriptase (RT) plays a critical role in holding and appropriately positioning the template-primer both at the polymerase active site and at the RNase H active site. For RT to successfully copy the vi...

journal_title：Journal of molecular biology

authors： Gao HQ,Boyer PL,Arnold E,Hughes SH

更新日期：1998-04-03 00:00:00

abstract：:Efficacy and sequence specificity are two major requirements in the use of antisense nucleic acids and ribozymes. For long-chain complementary RNA sequences (>30 nt), effects in living cells are correlated with the association rate of the complementary RNA in vitro, but not with the stability of the formed double stra...

journal_title：Journal of molecular biology

authors： Kronenwett R,Haas R,Sczakiel G

更新日期：1996-06-21 00:00:00

abstract：:Prion diseases are assumed to be caused by the infectious isoform, PrPsc, of a single cellular surface protein, PrPc. PrPsc is an insoluble form of PrPc and is believed to possess a different three-dimensional fold. It may propagate by causing PrPc to adopt its own infectious conformation by an unknown mechanism. Stud...

journal_title：Journal of molecular biology

authors： Hornemann S,Glockshuber R

更新日期：1996-09-06 00:00:00

abstract：:By expressing a mutant trpR gene in an Escherichia coli strain that is trpR and has beta-galactosidase activity fused to the trp promoter/operator, thus putting the beta-galactosidase activity under the control of the Trp repressor, we can determine quantitatively the relative repression activity of such mutant(s). We...

journal_title：Journal of molecular biology

authors： Storbakk N,Fenton C,Riise HM,Nilsen IW,El-Gewely MR

更新日期：1996-03-15 00:00:00

abstract：:Single crystals of the soluble glucose dehydrogenase (GDH) from Acinetobacter calcoaceticus have been grown by the vapour diffusion method. These crystals diffract to beyond 2.1 A and are suitable for X-ray crystallography. The space group was determined to be P2(1) with unit cell parameters a = 55.5 A, b = 104.5 A, c...

journal_title：Journal of molecular biology

authors： Schlunegger MP,Grütter MG,Streiff MB,Olsthoorn AJ,Duine JA

更新日期：1993-10-20 00:00:00

abstract：:The regulation of disulphide stress in actinomycetes such as Streptomyces coelicolor is known to involve the zinc-containing anti-sigma factor RsrA that binds and inactivates the redox-regulated sigma factor sigmaR. However, it is not known how RsrA senses disulphide stress nor what role the metal ion plays. Using in ...

journal_title：Journal of molecular biology

authors： Li W,Bottrill AR,Bibb MJ,Buttner MJ,Paget MS,Kleanthous C

更新日期：2003-10-17 00:00:00

abstract：:Single crystals of ribonuclease I from Escherichia coli have been obtained by the vapor diffusion method. The crystals belong to the tetragonal space group P4(1)2(1)2 or its enantiomer P4(3)2(1)2 and have cell parameters a = b = 119.01 A and c = 34.40 A. There is one 27,000 dalton monomer in the asymmetric unit. The c...

journal_title：Journal of molecular biology

authors： Lim LW,Mathur S,Cannistraro VJ,Kennell D

更新日期：1993-11-20 00:00:00

abstract：:Allosteric regulatory processes are implicated at all levels of biological function. Recent advances in our understanding of the diverse and functionally significant class of intrinsically disordered proteins have identified a multitude of ways in which disordered proteins function within the confines of the allosteri...

journal_title：Journal of molecular biology

authors： Berlow RB,Dyson HJ,Wright PE

更新日期：2018-08-03 00:00:00

abstract：:The kinetics of the signal recognition particle(SRP)-mediated process of protein translocation across the endoplasmic reticulum membrane was studied by mathematical modeling and complementary experiments. The following results were obtained. (1) A model according to which SRP directs the ribosome, rather than the mRNA...

journal_title：Journal of molecular biology

authors： Rapoport TA,Heinrich R,Walter P,Schulmeister T

更新日期：1987-06-05 00:00:00

abstract：:The plague-causing bacterium Yersinia pestis utilizes a type III secretion system to deliver effector proteins into mammalian cells where they interfere with signal transduction pathways that mediate phagocytosis and the inflammatory response. Effector proteins are injected through a hollow needle structure composed o...

journal_title：Journal of molecular biology

authors： Sun P,Tropea JE,Austin BP,Cherry S,Waugh DS

更新日期：2008-03-28 00:00:00

abstract：:Human serum albumin (HSA) is an abundant plasma protein that transports a wide variety of drugs and endogenous compounds. The complex binding capacity of HSA has made it a challenging system to study in detail but in order to develop our understanding of the interactions between ligands for HSA, the locations and rela...

journal_title：Journal of molecular biology

authors： Simard JR,Zunszain PA,Hamilton JA,Curry S

更新日期：2006-08-11 00:00:00

abstract：:Understanding the interaction of Arf and Hdm2 has recently become a central issue in cancer biology. In response to hyperproliferative signals, p14(Arf) stabilizes p53 by binding to Hdm2 and inhibits the ubiquitination and subsequent proteosome-dependent degradation of p53. The medical importance of the Arf-Hdm2-p53 r...

journal_title：Journal of molecular biology

authors： Bothner B,Lewis WS,DiGiammarino EL,Weber JD,Bothner SJ,Kriwacki RW

更新日期：2001-11-23 00:00:00

abstract：:Phenol-soluble modulin α3 (PSMα3) is a functional amyloid secreted by the pathogenic bacterium Staphylococcus aureus. This 22-residue peptide serves as a key virulence determinant, toxic to human cells via the formation of unique cross-α amyloid-like fibrils. We demonstrate that bilayer vesicles accelerated PSMα3 fibr...

journal_title：Journal of molecular biology

authors： Malishev R,Tayeb-Fligelman E,David S,Meijler MM,Landau M,Jelinek R

更新日期：2018-05-11 00:00:00

abstract：:The powerful explanatory paradigm of molecular biology requiring form to co-evolve with function has again been proven successful when, over the recent two decades, a wealth of biological functions have been uncovered for RNA. Previously considered as a mere mediator of the genetic code, RNA is now acknowledged as a k...

journal_title：Journal of molecular biology

authors： Hermann T,Patel DJ

更新日期：1999-12-10 00:00:00

abstract：:Structural analysis of delta131delta, a fragment model of the denatured state of staphylococcal nuclease, has been extended by obtaining long-range distance restraints between chain segments by paramagnetic relaxation enhancement. Fourteen unique PROXYL spin labels were introduced at sites that are solvent-exposed in ...

journal_title：Journal of molecular biology

authors： Gillespie JR,Shortle D

更新日期：1997-04-25 00:00:00