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The alkali molten globule state of horse ferricytochrome c: observation of cold denaturation.

Abstract：

:Here, we present the basic structural properties and the thermodynamic description of a previously unknown alkali molten globule state of horse "ferricytochrome c". Both sodium and guanidinium cations stabilize the alkali-denatured state at pH 13, presumably by a charge screening mechanism. The Na(+)-stabilized conformation (B state) clearly meets with the molecular organizational definition of the generic molten globule state. The B state exhibits highly cooperative thermal unfolding transitions monitored by both near and far-UV CD. Analyses of these transitions show substantial heat capacity change, suggesting that the hydrophobic effect contributes considerably to its energetic stability. At low salt concentration where molten globules are less stable, the B state undergoes reversible cold denaturation.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Kumar R,Prabhu NP,Rao DK,Bhuyan AK

doi

10.1016/j.jmb.2006.09.025

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

483-95

issue

3

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(06)01216-2

journal_volume

364

pub_type

杂志文章

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