SecA, the motor of the secretion machine, binds diverse partners on one interactive surface.

abstract：:We have determined the nucleotide sequence of three positive single-stranded RNA coliphages and have used this information, together with the known sequences of the related phages Q beta and SP, to construct a secondary structure model for the two distal domains of Q beta RNA. The 3' terminal domain, which is about 10...

journal_title：Journal of molecular biology

authors： Beekwilder MJ,Nieuwenhuizen R,van Duin J

更新日期：1995-04-14 00:00:00

abstract：:Membrane-bound proteases are involved in various regulatory functions. Our previous study indicated that the N-terminal region of an open reading frame, PH1510 (residues 16-236, designated as 1510-N) from the hyperthermophilic archaeon Pyrococcus horikoshii, is a serine protease with a catalytic Ser-Lys dyad that spec...

journal_title：Journal of molecular biology

authors： Yokoyama H,Matsui E,Akiba T,Harata K,Matsui I

更新日期：2006-05-12 00:00:00

abstract：:We developed a novel genetic method for finding functional regions of a protein by the analysis of chimeras formed between homologous proteins. Sets of chimeric genes were made by intramolecular homologous recombination in a linearized plasmid DNA carrying both recA genes of Escherichia coli and Pseudomonas aeruginosa...

journal_title：Journal of molecular biology

authors： Ogawa T,Shinohara A,Ogawa H,Tomizawa J

更新日期：1992-08-05 00:00:00

abstract：:Phage Mu transposes promiscuously, employing protein MuB for target capture. MuB forms stable filaments on A/T-rich DNA, and a correlation between preferred MuB binding and Mu integration has been observed. We have investigated the relationship between MuB-binding and Mu insertion into hot and cold Mu targets within t...

journal_title：Journal of molecular biology

authors： Ge J,Harshey RM

更新日期：2008-07-18 00:00:00

abstract：:As a test of the hypothesis that folding mechanisms are better conserved than sequences in TIM barrels, the equilibrium and kinetic folding mechanisms of indole-3-glycerol phosphate synthase (sIGPS) from the thermoacidophilic archaebacterium Sulfolobus solfataricus were compared to the well-characterized models of the...

journal_title：Journal of molecular biology

authors： Forsyth WR,Matthews CR

更新日期：2002-07-26 00:00:00

abstract：:Eukaryotic replication origins are activated at different times during the S phase of the cell cycle, following a temporal program that is stably transmitted to daughter cells. Although the mechanisms that control initiation at the level of individual origins are now well understood, much less is known on how cells co...

journal_title：Journal of molecular biology

authors： Yoshida K,Poveda A,Pasero P

更新日期：2013-11-29 00:00:00

abstract：:Sirtuins are NAD+-dependent protein deacetylase enzymes that are broadly conserved from bacteria to human, and have been implicated to play important roles in gene regulation, metabolism and longevity. cobB is a bacterial sirtuin that deacetylates acetyl-CoA synthetase (Acs) at an active site lysine to stimulate its e...

journal_title：Journal of molecular biology

authors： Zhao K,Chai X,Marmorstein R

更新日期：2004-03-26 00:00:00

abstract：:DNA polymerases that initiate replication by protein-priming are able to catalyze terminal protein (TP)-primed initiation, the following transition steps and finally DNA-primed elongation. Therefore, their structures must be able to position sequentially both primers, TP and DNA, at a common binding site. For DNA-temp...

journal_title：Journal of molecular biology

authors： Truniger V,Lázaro JM,Blanco L,Salas M

更新日期：2002-04-19 00:00:00

abstract：:Hepatocyte nuclear factor 1 (HNF1) is a dimeric homeoprotein expressed in hepatocytes and in a few other epithelial cells where it helps regulate the expression of a specific subset of genes. In an attempt to identify novel target genes for HNF1 and to assess the distribution of its target sites within the vertebrate ...

journal_title：Journal of molecular biology

authors： Tronche F,Ringeisen F,Blumenfeld M,Yaniv M,Pontoglio M

更新日期：1997-02-21 00:00:00

abstract：:Histone deacetylases (HDACs) modify core histones and participate in large regulatory complexes that both suppress and enhance transcription. Recent studies indicate that some HDACs can act on non-histone proteins as well. Interest in these enzymes is growing because HDAC inhibitors appear to be promising therapeutic ...

journal_title：Journal of molecular biology

authors： Gregoretti IV,Lee YM,Goodson HV

更新日期：2004-04-16 00:00:00

abstract：:The effects of several agents, sugars, isotonic KCl, and a variety of drugs, on the structure of the axonal membranes of unmyelinated pike olfactory nerve have been studied by synchrotron radiation X-ray scattering experiments. The main effects of the sugars are: (i) to increase the electron density of the extra-axona...

journal_title：Journal of molecular biology

authors： Luzzati V,Benoit E,Charpentier G,Vachette P

更新日期：2004-10-08 00:00:00

abstract：:Proteomics research has delivered many novel tumor targets. However, due to key limitations, it does not specifically identify targets that are most accessible for drug delivery, such as cell-surface antigens. A novel tumor antigen discovery platform based on screening a single domain antibody (sdAb) library against t...

journal_title：Journal of molecular biology

authors： Zhang J,Li Q,Nguyen TD,Tremblay TL,Stone E,To R,Kelly J,Roger MacKenzie C

更新日期：2004-07-30 00:00:00

abstract：:Insertion sequence IS1 contains two reading frames, insA and B'-insB, which are responsible for its transposition, and was previously shown to express two proteins. The first, InsA, is the product of insA. The second, InsA-B'-InsB is a fusion of InsA with the product of B'-insB. Synthesis of this protein occurs by a -...

journal_title：Journal of molecular biology

authors： Matsutani S

更新日期：1994-07-01 00:00:00

abstract：:Four previously isolated mutations in Salmonella phage P22 tailspike protein were used to study the relationship between protein stability, folding, and function. Tailspike protein binds and hydrolyzes the repetitive O-antigen structure in Salmonella lipopolysaccharide. Four mutations (V331G, V331A, A334V, A334I) are ...

journal_title：Journal of molecular biology

authors： Baxa U,Steinbacher S,Weintraub A,Huber R,Seckler R

更新日期：1999-10-29 00:00:00

abstract：:The structure and function of the apolipoproteins are of interest because of their central role in lipid metabolism. We report the complete primary structure of the human apo AII and CIII genes. These, like apo AI, contain three introns located at conserved positions. This may reflect their evolutionary and functional...

journal_title：Journal of molecular biology

authors： Shelley CS,Sharpe CR,Baralle FE,Shoulders CC

更新日期：1985-11-05 00:00:00

abstract：:The interaction sites of rabbit skeletal troponin I (TnI) with troponin C (TnC), troponin T (TnT), tropomyosin (Tm) and actin were mapped systematically using nine single cysteine residue TnI mutants with mutation sites at positions 6, 48, 64, 89, 104, 121, 133, 155 or 179 (TnI6, TnI48 etc.). Each mutant was labeled w...

journal_title：Journal of molecular biology

authors： Luo Y,Wu JL,Li B,Langsetmo K,Gergely J,Tao T

更新日期：2000-02-25 00:00:00

abstract：:Crystals of NADPH:FMN oxidoreductase from Vibrio harveyi have been obtained and characterized by X-ray diffraction. This enzyme plays a role in the generation of light in luminescent bacteria by providing reduced FMN to luciferase. Large, high quality crystals were grown using polyethylene glycol 6000 at pH 7.0. They ...

journal_title：Journal of molecular biology

authors： Tanner J,Lei B,Liu M,Tu SC,Krause KL

更新日期：1994-08-12 00:00:00

abstract：:Progress in the structure determination of bacteriorhodopsin, the protein component of purple membrane from Halobacterium halobium has been limited by the lack of three-dimensional phase information between 6 and 3 A resolution. By analogy with X-ray methods, it is possible that heavy-atom labelling of the membrane cr...

journal_title：Journal of molecular biology

authors： Ceska TA,Henderson R

更新日期：1990-06-05 00:00:00

abstract：:Nuclear protein-coding genes of euglenids (Discoba, Euglenozoa, Euglenida) contain conventional (spliceosomal) and nonconventional introns. The latter have been found only in euglenozoans. A unique feature of nonconventional introns is the ability to form a stable and slightly conserved RNA secondary structure bringin...

journal_title：Journal of molecular biology

authors： Gumińska N,Zakryś B,Milanowski R

更新日期：2021-02-05 00:00:00

abstract：:Methyltransferases (MTases) from the Erm family catalyze S-adenosyl-L-methionine-dependent modification of a specific adenine residue in bacterial 23S rRNA, thereby conferring resistance to clinically important macrolide, lincosamide and streptogramin B antibiotics. Despite the available structural data and functional...

journal_title：Journal of molecular biology

authors： Maravić G,Feder M,Pongor S,Flögel M,Bujnicki JM

更新日期：2003-09-05 00:00:00

abstract：:Antibody Z13e1 is a relatively broadly neutralizing anti-human immunodeficiency virus type 1 antibody that recognizes the membrane-proximal external region (MPER) of the human immunodeficiency virus type 1 envelope glycoprotein gp41. Based on the crystal structure of an MPER epitope peptide in complex with Z13e1 Fab, ...

journal_title：Journal of molecular biology

authors： Stanfield RL,Julien JP,Pejchal R,Gach JS,Zwick MB,Wilson IA

更新日期：2011-12-02 00:00:00

abstract：:Transcription of the rabbit alpha-globin gene begins primarily at the cap site, although some upstream start sites are also observed. Analysis by RNA polymerase run-on assays in nuclei shows that transcription continues at a high level past the polyadenylation site, after which the polymerase density actually increase...

journal_title：Journal of molecular biology

authors： Vandenbergh DJ,James-Pederson M,Hardison RC

更新日期：1991-07-20 00:00:00

abstract：:Escherichia coli pyridoxine 5'-phosphate oxidase catalyzes the terminal step in the biosynthesis of pyridoxal 5'-phosphate by the FMN oxidation of pyridoxine 5'-phosphate forming FMNH(2) and H(2)O(2). Recent studies have shown that in addition to the active site, pyridoxine 5'-phosphate oxidase contains a non-catalyti...

journal_title：Journal of molecular biology

authors： Safo MK,Musayev FN,di Salvo ML,Schirch V

更新日期：2001-07-20 00:00:00

abstract：:Muscular dystrophies arise with various mutations in dystrophin, implicating this protein in force transmission in normal muscle. With 24 three-helix, spectrin repeats interspersed with proline-rich hinges, dystrophin's large size is an impediment to gene therapy, prompting the construction of mini-dystrophins. Result...

journal_title：Journal of molecular biology

authors： Bhasin N,Law R,Liao G,Safer D,Ellmer J,Discher BM,Sweeney HL,Discher DE

更新日期：2005-09-30 00:00:00

abstract：:Cyclins and cyclin-dependent kinases (CDKs) are key components of signaling pathways essential for cell growth and survival. The cyclin-CDK Pho80-Pho85 inactivates the transcription factor Pho4 in budding yeast by phosphorylating it on five sites. We isolated seven single amino acid substitutions outside of the phosph...

journal_title：Journal of molecular biology

authors： Byrne M,Miller N,Springer M,O'Shea EK

更新日期：2004-01-02 00:00:00

abstract：:C-tailed membrane proteins insert into the bilayer post-translationally because the hydrophobic anchor segment leaves the ribosome at the end of translation. Nevertheless, we find here evidence that the targeting of SciP to the membrane of Escherichia coli occurs co-translationally since signal elements in the N-termi...

journal_title：Journal of molecular biology

authors： Pross E,Soussoula L,Seitl I,Lupo D,Kuhn A

更新日期：2016-10-09 00:00:00

abstract：:beta-Glucosyltransferase (BGT) is a DNA-modifying enzyme encoded by bacteriophage T4 that transfers glucose from uridine diphosphoglucose to 5-hydroxymethyl cytosine bases of phage T4 DNA. We report six X-ray structures of the substrate-free and the UDP-bound enzyme. Four also contain metal ions which activate the enz...

journal_title：Journal of molecular biology

authors： Moréra S,Larivière L,Kurzeck J,Aschke-Sonnenborn U,Freemont PS,Janin J,Rüger W

更新日期：2001-08-17 00:00:00

abstract：:Vinculin is a key regulator of the actin cytoskeleton attachment to the cell membrane at cellular adhesion sites, which is crucial for processes such as cell motility and migration, development, survival, and wound healing. Vinculin loss results in embryonic lethality, cardiovascular diseases, and cancer. Its tail dom...

journal_title：Journal of molecular biology

authors： Lee JH,Rangarajan ES,Vonrhein C,Bricogne G,Izard T

更新日期：2012-10-05 00:00:00

abstract：:We have previously described methodology that makes it possible to genetically encode a wide array of unnatural amino acids in both prokaryotic and eukaryotic organisms. Here, we report the systematic optimization of a Saccharomyces cerevisiae expression system for the production of mutant proteins containing unnatura...

journal_title：Journal of molecular biology

authors： Chen S,Schultz PG,Brock A

更新日期：2007-08-03 00:00:00

abstract：:Protein-protein interactions within the membrane, partially or fully mediated by transmembrane (TM) domains, are involved in many vital cellular processes. Since the unique feature of the membrane environment enables protein-protein assembly that otherwise is not energetically favored in solution, the structural restr...

journal_title：Journal of molecular biology

authors： Sal-Man N,Gerber D,Shai Y

更新日期：2004-11-26 00:00:00