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Crystallization and preliminary crystallographic analysis of NADPH:FMN oxidoreductase from Vibrio harveyi.

Abstract：

:Crystals of NADPH:FMN oxidoreductase from Vibrio harveyi have been obtained and characterized by X-ray diffraction. This enzyme plays a role in the generation of light in luminescent bacteria by providing reduced FMN to luciferase. Large, high quality crystals were grown using polyethylene glycol 6000 at pH 7.0. They crystallize in the monoclinic space group P2(1) with cell dimensions a = 51.2 A, b = 85.9 A, c = 58.1 A, beta = 109.3 degrees, and diffract to 1.8 A. We expect two molecules per asymmetric unit. High resolution data sets have been recorded and a search is under way for heavy-atom derivatives.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Tanner J,Lei B,Liu M,Tu SC,Krause KL

doi

10.1006/jmbi.1994.1501

subject

Has Abstract

pub_date

1994-08-12 00:00:00

pages

283-7

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(84)71501-4

journal_volume

241

pub_type

杂志文章

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