Transcription from the rha operon psr promoter.

abstract：:A maltodextrin-binding protein from Pyrococcus furiosus (PfuMBP) has been overproduced in Escherichia coli, purified, and crystallized. The crystal structure of the protein bound to an oligosaccharide ligand was determined to 1.85 A resolution. The fold of PfuMBP is very similar to that of the orthologous MBP from E. ...

journal_title：Journal of molecular biology

authors： Evdokimov AG,Anderson DE,Routzahn KM,Waugh DS

更新日期：2001-01-26 00:00:00

abstract：:dCMP-deaminase-deficient V79/dC hamster cells have highly imbalanced deoxyribonucleoside triphosphate (dNTP) pools, i.e. a 17-fold larger dCTP pool, a slightly reduced dTTP and a very low dGTP pool, compared to dCMP-deaminase-proficient V79/p cells. Nevertheless, the two lines showed the same rates of spontaneous muta...

journal_title：Journal of molecular biology

authors： Darè E,Zhang LH,Jenssen D,Bianchi V

更新日期：1995-10-06 00:00:00

abstract：:Four new crystal forms of the anti-T lectin from jackfruit (Artocarpus integrifolia) have been prepared and characterized. Three of them, two monoclinic (P21, a = 59.4 A, b = 83.3 A, c = 63.5 A, beta = 107.7 degrees; C2, a = 106.1 A, b = 53.9 A, c = 128.0 A, beta = 95.0 A) and one orthorhombic (C222(1), a = 98.1 A, b ...

journal_title：Journal of molecular biology

authors： Banerjee R,Dhanaraj V,Mahanta SK,Surolia A,Vijayan M

更新日期：1991-10-05 00:00:00

abstract：:Small RNAs were selected from a highly degenerate library on the basis of their ability to bind tightly to Escherichia coli phenylalanyl-tRNA synthetase (FRS). The 63 nucleotide library consisted of the acceptor stem and portions of the D and T stems of E. coli tRNA(Phe) flanking a 32 nucleotide randomized region. Bec...

journal_title：Journal of molecular biology

authors： Peterson ET,Pan T,Coleman J,Uhlenbeck OC

更新日期：1994-09-23 00:00:00

abstract：:An understanding of the structural basis for protein-protein interactions, and molecular recognition in general, requires complete characterization of binding energetics. Not only does this include quantification of the changes that occur in all of the thermodynamic parameters upon binding, including the enthalpy, ent...

journal_title：Journal of molecular biology

authors： Baker BM,Murphy KP

更新日期：1997-05-02 00:00:00

abstract：:The first naturally occurring split intein was found in the dnaE gene of Synechocystis sp. PCC6803 and belongs to a subclass of inteins without a penultimate histidine residue. We describe two high-resolution crystal structures, one derived from an excised Ssp DnaE intein and the second from a splicing-deficient precu...

journal_title：Journal of molecular biology

authors： Sun P,Ye S,Ferrandon S,Evans TC,Xu MQ,Rao Z

更新日期：2005-11-11 00:00:00

abstract：:We report the solution of the crystal structure of a mutant of the immunoglobulin VL domain of the antibody McPC603, in which the complementarity-determining region 1 segment is replaced with that of a different antibody. The wild-type and mutant crystal structures have been refined to a crystallographic R-factor of 1...

journal_title：Journal of molecular biology

authors： Steipe B,Plückthun A,Huber R

更新日期：1992-06-05 00:00:00

abstract：:UL9 protein and ICP8 encoded by the herpes simplex virus type 1 (HSV-1) were shown to catalyze a highly active, non-origin-dependent unwinding of DNA. UL9 protein, the HSV-1 origin binding protein, as a modest helicase activity that is greatly stimulated by the HSV-1 single strand (ss) binding protein, ICP8. Here, ele...

journal_title：Journal of molecular biology

authors： Makhov AM,Boehmer PE,Lehman IR,Griffith JD

更新日期：1996-05-24 00:00:00

abstract：:The endA gene encoding the membrane nuclease of Streptococcus pneumoniae, which is necessary for DNA uptake in genetic transformation, was cloned in a streptococcal vector. This was accomplished by insertional mutagenesis of the gene, cloning of the mutant allele, and substitution of the wild-type allele by chromosoma...

journal_title：Journal of molecular biology

authors： Puyet A,Greenberg B,Lacks SA

更新日期：1990-06-20 00:00:00

abstract：:Ribosomes are large nucleoproteins of approximately 3 X 10(6) Mr. In contrast to helical or spherical nucleoproteins (viruses) of similar size (which consist of several hundred small asymmetric units reproduced by symmetry), ribosomes are completely asymmetric; therefore, the amount of structural information (defined ...

journal_title：Journal of molecular biology

authors： Hoppe W,Oettl H,Tietz HR

更新日期：1986-11-20 00:00:00

abstract：:We used site-specific recombination catalyzed by the bacteriophage lambda Int system to probe DNA structure and metabolism in vivo. In vitro, the complexity of catenated products was linearly proportional to substrate supercoil density. A system was developed that gave efficient, controlled Int recombination in Escher...

journal_title：Journal of molecular biology

authors： Bliska JB,Cozzarelli NR

更新日期：1987-03-20 00:00:00

abstract：:Recombination between RNAs associated with turnip crinkle virus is thought to occur during plus-strand synthesis at motifs resembling the 5'-ends of genomic, subgenomic and satellite RNAs. Common structural regions encompassing the motifs have been found for major crossover sites on two different minus-strand template...

journal_title：Journal of molecular biology

authors： Carpenter CD,Oh JW,Zhang C,Simon AE

更新日期：1995-02-03 00:00:00

abstract：:We have studied the use of nearest-neighbor classifiers to predict the secondary structure of proteins. The nearest-neighbor rule states that a test instance is classified according to the classifications of "nearby" training examples from a database of known structures. In the context of secondary structure predictio...

journal_title：Journal of molecular biology

authors： Yi TM,Lander ES

更新日期：1993-08-20 00:00:00

abstract：:The evolution of protein interactions cannot be deciphered without a detailed analysis of interaction interfaces and binding modes. We performed a large-scale study of protein homooligomers in terms of their symmetry, interface sizes, and conservation of binding modes. We also focused specifically on the evolution of ...

journal_title：Journal of molecular biology

authors： Dayhoff JE,Shoemaker BA,Bryant SH,Panchenko AR

更新日期：2010-01-29 00:00:00

abstract：:GTP and Ca2+, two well-known modulators of intracellular signaling pathways, control a structural/functional switch between two vital and mutually exclusive activities, cross-linking and Galpha activity, in the same enzyme. The enzyme, a brain-derived tissue-type transglutaminase (TGase), was recently cloned by us in ...

journal_title：Journal of molecular biology

authors： Monsonego A,Friedmann I,Shani Y,Eisenstein M,Schwartz M

更新日期：1998-10-02 00:00:00

abstract：:Staphylococcus aureus is an opportunistic human pathogen able to transfer virulence genes to other cells through the mobilization of S. aureus pathogenicity islands (SaPIs). SaPIs are derepressed and packaged into phage-like transducing particles by helper phages like 80α or φNM1. Phages 80α and φNM1 encode structural...

journal_title：Journal of molecular biology

authors： Hill RLL,Vlach J,Parker LK,Christie GE,Saad JS,Dokland T

更新日期：2017-05-19 00:00:00

abstract：:The assignment of the 1H nuclear magnetic resonance (n.m.r.) spectrum of the protease inhibitor IIA from bull seminal plasma is described and documented. The assignments are based entirely on the amino acid sequence and on two-dimensional n.m.r. experiments at 500 MHz. Individual assignments were obtained at 18 degree...

journal_title：Journal of molecular biology

authors： Strop P,Wider G,Wüthrich K

更新日期：1983-06-05 00:00:00

abstract：:The structure of tobacco mosaic virus (TMV) has been determined by fiber diffraction methods at 2.9 A resolution, and refined by restrained least-squares to an R-factor of 0.096. Protein-nucleic acid interactions are clearly visible. The final model contains all of the non-hydrogen atoms of the RNA and the protein, 71...

journal_title：Journal of molecular biology

authors： Namba K,Pattanayek R,Stubbs G

更新日期：1989-07-20 00:00:00

abstract：:Native proteins exhibit precise geometric packing of atoms in their hydrophobic interiors. Nonetheless, controversy remains about the role of core side-chain packing in specifying and stabilizing the folded structures of proteins. Here we investigate the role of core packing in determining the conformation and stabili...

journal_title：Journal of molecular biology

authors： Liu J,Cao W,Lu M

更新日期：2002-05-03 00:00:00

abstract：:Microtubule (MT) stability is related to the hydrolysis of the guanosine triphosphate nucleotide (NT) bound to β-tubulin. However, the molecular mechanism by which the NT state influences the stability of the contacts in the MT lattice remains elusive. Here, we present large-scale atomistic simulations of different tu...

journal_title：Journal of molecular biology

authors： Grafmüller A,Noya EG,Voth GA

更新日期：2013-06-26 00:00:00

abstract：:The ctc gene of Bacillus subtilis is transcribed in vitro by the minor RNA polymerase holoenzyme forms, E sigma 37 and E sigma 32. To study the expression and regulation of ctc in vivo, we constructed operon and translational fusions of the ctc promoter region to the lacZ gene of Escherichia coli. Our results indicate...

journal_title：Journal of molecular biology

authors： Igo MM,Losick R

更新日期：1986-10-20 00:00:00

abstract：:A protein-protein Förster resonance energy transfer (FRET) system, employing probes at multiple positions, was designed to specifically monitor the dissociation of the H2A-H2B dimer from the nucleosome core particle (NCP). Tryptophan donors and Cys-AEDANS acceptors were chosen because, compared to previous NCP FRET fl...

journal_title：Journal of molecular biology

authors： Hoch DA,Stratton JJ,Gloss LM

更新日期：2007-08-24 00:00:00

abstract：:Low dose electron diffraction and imaging techniques have been applied to the study of the crystalline structure of gp32*I, a DNA helix destabilizing protein derived from bacteriophage T4 gene 32 protein. A quantitative analysis of intensities from electron diffraction patterns from tilted, multilayered gp32*I crystal...

journal_title：Journal of molecular biology

authors： Cohen HA,Chiu W,Hosoda J

更新日期：1983-09-05 00:00:00

abstract：:Oligonucleotides can bind to double-stranded DNA in a sequence-specific manner to form triple helices. Uniformly modified, pyrimidine-rich oligodeoxyribonuclotides containing internucleosidic N3'-P5' phosphoramidate linkages are known to form very stable triplexes with their DNA target. Psoralen-conjugated triple heli...

journal_title：Journal of molecular biology

authors： Faria M,Wood CD,White MR,Hélène C,Giovannangeli C

更新日期：2001-02-09 00:00:00

abstract：:Escherichia coli thioesterase I (TAP) is a multifunctional enzyme possessing activities of thioesterase, esterase, arylesterase, protease, and lysophospholipase. In particular, TAP has stereoselectivity for amino acid derivative substrates, hence it is useful for the kinetic resolution of racemic mixtures of industria...

journal_title：Journal of molecular biology

authors： Lo YC,Lin SC,Shaw JF,Liaw YC

更新日期：2003-07-11 00:00:00

abstract：:The LH1 light harvesting complex has been purified from a mutant of the photosynthetic bacterium Rhodobacter sphaeroides which synthesizes LH1 as the sole pigment protein. Crystallization trials using polyethylene glycol as the precipitant in the presence of the detergent n-octyl glucoside have resulted in the formati...

journal_title：Journal of molecular biology

authors： Nunn RS,Artymiuk PJ,Baker PJ,Rice DW,Hunter CN

更新日期：1992-12-20 00:00:00

abstract：:Site-directed mutations were introduced in the connecting loops and one of the two stem regions of the RNA pseudoknot in the tRNA-like structure of turnip yellow mosaic virus RNA. The kinetic parameters of valylation for each mutated RNA were determined in a cell-free extract from wheat germ. Structure mapping was per...

journal_title：Journal of molecular biology

authors： Mans RM,Van Steeg MH,Verlaan PW,Pleij CW,Bosch L

更新日期：1992-01-05 00:00:00

abstract：:The extracellular matrix in tissues such as bone, tendon and cornea contains ordered, parallel arrays of collagen type I fibrils. Cells embedded in these matrices frequently co-align with the collagen fibrils, suggesting that ordered fibrils provide structural or signalling cues for cell polarization. To study mechani...

journal_title：Journal of molecular biology

authors： Friedrichs J,Taubenberger A,Franz CM,Muller DJ

更新日期：2007-09-21 00:00:00

abstract：:We have constructed complexes of ribosomal proteins S8, S15, S8 + S15 and S8 + S15 + S6 + S18 with 16 S ribosomal RNA, and probed the RNA moiety with a set of structure-specific chemical and enzymatic probes. Our results show the following effects of assembly of proteins on the reactivity of specific nucleotides in 16...

journal_title：Journal of molecular biology

authors： Svensson P,Changchien LM,Craven GR,Noller HF

更新日期：1988-03-20 00:00:00

abstract：:Essentially all cold agglutinins (CA) with red blood cell I/i specificity isolated from patients with CA disease stemming from lymphoproliferative disorders utilize the VH 4-34 (VH 4-21) gene segment. This near universality of the restricted use of a single gene segment is substantially greater than that demonstrated ...

journal_title：Journal of molecular biology

authors： Li Y,Spellerberg MB,Stevenson FK,Capra JD,Potter KN

更新日期：1996-03-01 00:00:00