当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Structural analysis of T4 DNA helix destabilizing protein (gp32 I) crystal by electron microscopy.

Structural analysis of T4 DNA helix destabilizing protein (gp32 I) crystal by electron microscopy.

Abstract：

:Low dose electron diffraction and imaging techniques have been applied to the study of the crystalline structure of gp32*I, a DNA helix destabilizing protein derived from bacteriophage T4 gene 32 protein. A quantitative analysis of intensities from electron diffraction patterns from tilted, multilayered gp32*I crystal has provided the unit cell thickness of the crystal. The three-dimensional phases indicate that the space group P2(1)2(1)2. By taking into account the unit cell volume and the solvent content in the crystal, it was deduced that there is one gp32*I molecule in each asymmetric unit. A projected density map of unstained, glucose-embedded gp32*I crystal was synthesized with amplitudes from electron diffraction intensities and phases from electron images with reflections out to 7.6 A. Because of the similarity in the scattering density between glucose and protein, this projected map cannot be interpreted with certainty. A low resolution three-dimensional reconstruction shows that the protein molecule is about 90 A long and about 20 A in diameter. Because the dimer is formed around a dyad axis, the protein molecules comprising it must be arranged head-to-head. This dimeric arrangement of the proteins in the unit cell may be implicated as one of the conformational states of this protein in solution.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Cohen HA,Chiu W,Hosoda J

doi

10.1016/s0022-2836(83)80182-x

subject

Has Abstract

pub_date

1983-09-05 00:00:00

pages

235-48

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(83)80182-X

journal_volume

169

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Transcriptional repressor CopR: the structured acidic C terminus is important for protein stability.

    abstract：:The transcriptional repressor CopR is one of the two copy-number control components of plasmid pIP501. CopR binds as a dimer at two consecutive major grooves on the same face of the DNA. Previously, equilibrium dissociation constants of CopR dimers and the CopR-DNA complex and the intracellular CopR concentration were...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3929

    authors： Kuhn K,Steinmetzer K,Brantl S

    更新日期：2000-07-28 00:00:00

  • Polymer chain statistics and conformational analysis of DNA molecules with bends or sections of different flexibility.

    abstract：:The worm-like chain model has often been employed to describe the average conformation of long, intrinsically straight polymer molecules, including DNA. The present study extends the applicability of the worm-like chain model to polymers containing bends or sections of different flexibility. Several cases have been ex...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.1830

    authors： Rivetti C,Walker C,Bustamante C

    更新日期：1998-07-03 00:00:00

  • Phosphorylation and mutation of phospholamban alter physical interactions with the sarcoplasmic reticulum calcium pump.

    abstract：:Phospholamban physically interacts with the sarcoplasmic reticulum calcium pump (SERCA) and regulates contractility of the heart in response to adrenergic stimuli. We studied this interaction using electron microscopy of 2D crystals of SERCA in complex with phospholamban. In earlier studies, phospholamban oligomers we...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.11.014

    authors： Glaves JP,Trieber CA,Ceholski DK,Stokes DL,Young HS

    更新日期：2011-01-21 00:00:00

  • Peroxisome proliferator-activated receptor alpha controls hepatic heme biosynthesis through ALAS1.

    abstract：:Heme is an essential prosthetic group of proteins involved in oxygen transport, energy metabolism and nitric oxide production. ALAS1 (5-aminolevulinate synthase) is the rate-limiting enzyme in heme synthesis in the liver and is highly regulated to adapt to the metabolic demand of the hepatocyte. In the present study, ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,收录出版

    doi：10.1016/j.jmb.2009.03.024

    authors： Degenhardt T,Väisänen S,Rakhshandehroo M,Kersten S,Carlberg C

    更新日期：2009-05-01 00:00:00

  • TissueCoCoPUTs: Novel Human Tissue-Specific Codon and Codon-Pair Usage Tables Based on Differential Tissue Gene Expression.

    abstract：:Protein expression in multicellular organisms varies widely across tissues. Codon usage in the transcriptome of each tissue is derived from genomic codon usage and the relative expression level of each gene. We created a comprehensive computational resource that houses tissue-specific codon, codon-pair, and dinucleoti...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.01.011

    authors： Kames J,Alexaki A,Holcomb DD,Santana-Quintero LV,Athey JC,Hamasaki-Katagiri N,Katneni U,Golikov A,Ibla JC,Bar H,Kimchi-Sarfaty C

    更新日期：2020-05-15 00:00:00

  • The three-dimensional structure at 2.4 A resolution of glycosylated proteinase A from the lysosome-like vacuole of Saccharomyces cerevisiae.

    abstract：:The crystal structures of glycosylated native proteinase A, an aspartic proteinase found in the vacuole of Saccharomyces cerevisiae, and its complex with a difluorostatone-containing tripeptide have been determined by molecular replacement to 3.5 A and 2.4 A resolutions, respectively. Superposition of the bound and na...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0880

    authors： Aguilar CF,Cronin NB,Badasso M,Dreyer T,Newman MP,Cooper JB,Hoover DJ,Wood SP,Johnson MS,Blundell TL

    更新日期：1997-04-11 00:00:00

  • Single molecule force spectroscopy of spectrin repeats: low unfolding forces in helix bundles.

    abstract：:Spectrin repeats fold into triple helical coiled-coils comprising approximately 106 amino acid residues. Using an AFM-related technique we measured the force required to mechanically unfold these repeats to be 25 to 35 pN. Under tension, individual spectrin repeats unfold independently and in an all-or-none process. T...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2466

    authors： Rief M,Pascual J,Saraste M,Gaub HE

    更新日期：1999-02-19 00:00:00

  • Structural and functional analysis of a human 7 S K RNA gene.

    abstract：:Using purified RNA from HeLa cells, we have synthesized and cloned a cDNA encoding an almost entire 7 S K RNA. This cDNA probe was used to isolate 7 S K RNA gene sequences from a human genomic library by high-stringency colony hybridization. In order to differentiate between functional genes and related sequences, we ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90325-1

    authors： Krüger W,Benecke BJ

    更新日期：1987-05-05 00:00:00

  • A thermodynamic and kinetic analysis of the folding pathway of an SH3 domain entropically stabilised by a redesigned hydrophobic core.

    abstract：:The folding thermodynamics and kinetics of the alpha-spectrin SH3 domain with a redesigned hydrophobic core have been studied. The introduction of five replacements, A11V, V23L, M25V, V44I and V58L, resulted in an increase of 16% in the overall volume of the side-chains forming the hydrophobic core but caused no remar...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00273-0

    authors： Cobos ES,Filimonov VV,Vega MC,Mateo PL,Serrano L,Martínez JC

    更新日期：2003-04-18 00:00:00

  • Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin.

    abstract：:The interplay between metal binding, carbohydrate binding activity, stability and structure of the lectin from Pterocarpus angolensis was investigated. Removal of the metals leads to a more flexible form of the protein with significantly less conformational stability. Crystal structures of this metal-free form show si...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.06.006

    authors： Garcia-Pino A,Buts L,Wyns L,Loris R

    更新日期：2006-08-04 00:00:00

  • Molecular Highways-Navigating Collisions of DNA Motor Proteins.

    abstract：:Fundamental biological processes require concurrent sharing of DNA by numerous motor proteins and complexes. Thus, collision, congestion, and roadblocks are inescapable on these busy "molecular highways." The consequences of these traffic problems are diverse, resulting in complex cellular mechanisms to resolve threat...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2018.08.006

    authors： Le TT,Wang MD

    更新日期：2018-10-26 00:00:00

  • Crystal structure of muconate lactonizing enzyme at 3 A resolution.

    abstract：:The crystal structure of muconate lactonizing enzyme has been solved at 3 A resolution, and an unambiguous alpha-carbon backbone chain trace made. The enzyme contains three domains; the central domain is a parallel-stranded alpha-beta barrel, which has previously been reported in six other enzymes, including triose ph...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90723-6

    authors： Goldman A,Ollis DL,Steitz TA

    更新日期：1987-03-05 00:00:00

  • Zinc-mediated Reversible Multimerization of Hsp31 Enhances the Activity of Holding Chaperone.

    abstract：:Hsp31 protein, belonging to the DJ-1/ThiJ/PfpI superfamily, increases the survival of Escherichia coli under various stresses. While it was reported as a holding chaperone, Hsp31 was also shown to exhibit the glyoxalase III activity in subsequent study. Here, we describe our finding that Hsp31 undergoes a Zn+2-mediate...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2018.04.029

    authors： Kim J,Choi D,Cha SY,Oh YM,Hwang E,Park C,Ryu KS

    更新日期：2018-06-08 00:00:00

  • Structurally constrained residues outside the binding motif are essential in the interaction of 14-3-3 and phosphorylated partner.

    abstract：:14-3-3 proteins participate in many key cellular processes after binding to disordered phospho-partners. Usually, the phosphorylated state is an essential target for the binding. Here, we show for the first time residues other than those in the 14-3-3 binding motif that are essential for the binding between 14-3-3 and...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.12.043

    authors： Uhart M,Iglesias AA,Bustos DM

    更新日期：2011-03-04 00:00:00

  • Supramolecular Assembly of Human Pulmonary Surfactant Protein SP-D.

    abstract：:Pulmonary surfactant protein D (SP-D) is a glycoprotein from the collectin family that is a component of the lung surfactant system. It exhibits host defense and immune regulatory functions in addition to contributing to the homeostasis of the surfactant pool within the alveolar airspaces. It is known that the SP-D mo...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2018.03.027

    authors： Arroyo R,Martín-González A,Echaide M,Jain A,Brondyk WH,Rosenbaum J,Moreno-Herrero F,Pérez-Gil J

    更新日期：2018-05-11 00:00:00

  • Synthesis and degradation of the mRNA of the Tn21 mer operon.

    abstract：:The mercury resistance locus encoded by Tn21 on the monocopy IncFII plasmid R100 (merTn21) consists of a metal-responsive activator/repressor, merR, which controls initiation of a polycistronic message that includes genes for the uptake (merTPC) and reduction (merA) of Hg2+ and merD, which may also play a minor regula...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90919-b

    authors： Gambill BD,Summers AO

    更新日期：1992-05-20 00:00:00

  • Analysis of putative RNase P RNA from orthopoxviruses.

    abstract：:A putative RNase P RNA gene in camelpox virus, one of the orthopoxviruses, was cloned and transcribed in vitro. No RNase P activity could be detected in vitro from camelpox virus RNase P RNA alone, or by addition of the Escherichia coli RNase P protein subunit to reaction mixtures. Camelpox virus RNase P RNA reconstit...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.09.020

    authors： Yang L,Wesolowski D,Li Y,Altman S

    更新日期：2005-12-02 00:00:00

  • Mutation spectrum following transfection of ultraviolet-irradiated single-stranded or double-stranded shuttle vector DNA into monkey cells.

    abstract：:We designed a shuttle vector system that allowed a comparison of the mutation spectrum on the supF target gene after transfection of single-stranded or double-stranded DNA into monkey cells. Single-strand-derived plasmids exhibited a spontaneous mutation frequency tenfold higher than double-strand-derived ones. These ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90252-2

    authors： Madzak C,Sarasin A

    更新日期：1991-04-20 00:00:00

  • Crystal structures of cobalamin-independent methionine synthase (MetE) from Streptococcus mutans: a dynamic zinc-inversion model.

    abstract：:Cobalamin-independent methionine synthase (MetE) catalyzes the direct transfer of a methyl group from methyltetrahydrofolate to l-homocysteine to form methionine. Previous studies have shown that the MetE active site coordinates a zinc atom, which is thought to act as a Lewis acid and plays a role in the activation of...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2011.08.005

    authors： Fu TM,Almqvist J,Liang YH,Li L,Huang Y,Su XD

    更新日期：2011-09-30 00:00:00

  • Antibody-antigen interactions: contact analysis and binding site topography.

    abstract：:We have analysed antigen-contacting residues and combining site shape in the antibody Fv and Fab crystal structures now available from the Protein Data Bank. Antigen-contacting propensities are presented for each antibody residue, allowing a new definition for the complementarity determining regions (CDRs) to be propo...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0548

    authors： MacCallum RM,Martin AC,Thornton JM

    更新日期：1996-10-11 00:00:00

  • Self-splicing of a Podospora anserina group IIA intron in vitro. Effects of 3'-terminal intron alterations on cleavage at the 5' and 3' splice site.

    abstract：:A shortened derivative of the group IIA intron from the mitochondrial cytochrome-c-oxidase subunit I gene (COI I1) of the ascomycete Podospora anserina can undergo self-splicing in vitro. When compared to self-splicing group IIB introns from yeast mitochondria (aI5c, bI1) the autocatalytic reaction shows a lower effic...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1309

    authors： Schmidt U,Sägebarth R,Schmelzer C,Stahl U

    更新日期：1993-06-05 00:00:00

  • Mechanism and energy landscape of domain swapping in the B1 domain of protein G.

    abstract：:Three-dimensional domain swapping has emerged as a ubiquitous process for homo-oligomer formation in many unrelated proteins, but the molecular mechanism of this process is still poorly understood. Here we present a mechanism for the swapping reaction in the B1 domain of the immunoglobulin G binding protein from group...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.06.025

    authors： Malevanets A,Sirota FL,Wodak SJ

    更新日期：2008-09-26 00:00:00

  • Structure and mechanism of the magnesium-independent aromatic prenyltransferase CloQ from the clorobiocin biosynthetic pathway.

    abstract：:CloQ is an aromatic prenyltransferase from the clorobiocin biosynthetic pathway of Streptomyces roseochromogenes var. oscitans. It is involved in the synthesis of the prenylated hydroxybenzoate moiety of the antibiotic, specifically catalyzing the attachment of a dimethylallyl moiety to 4-hydroxyphenylpyruvate. Herein...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.09.067

    authors： Metzger U,Keller S,Stevenson CE,Heide L,Lawson DM

    更新日期：2010-12-10 00:00:00

  • ATP synthase from bovine heart mitochondria. In vitro assembly of a stalk complex in the presence of F1-ATPase and in its absence.

    abstract：:Four subunits of the F1F0-ATPase from bovine heart mitochondria have been produced by heterologous over-expression in Escherichia coli. They are the oligomycin sensitivity conferral protein (OSCP), coupling factor 6 (F6) and subunits b and d. Likewise, fragments b', bI, bC, and bM (amino acid residues 79 to 214, 121 t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1591

    authors： Collinson IR,van Raaij MJ,Runswick MJ,Fearnley IM,Skehel JM,Orriss GL,Miroux B,Walker JE

    更新日期：1994-09-30 00:00:00

  • Atomic resolution (1.0 A) crystal structure of Fusarium solani cutinase: stereochemical analysis.

    abstract：:X-ray data have been recorded to 1.0 A resolution from a crystal of Fusarium solani cutinase using synchrotron radiation and an imaging-plate scanner. The anisotropic treatment of thermal motion led to a fivefold increase in accuracy and to a considerable quality improvement in the electron density maps with respect t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1000

    authors： Longhi S,Czjzek M,Lamzin V,Nicolas A,Cambillau C

    更新日期：1997-05-16 00:00:00

  • X-ray structure of the quinoprotein ethanol dehydrogenase from Pseudomonas aeruginosa: basis of substrate specificity.

    abstract：:The homodimeric enzyme form of quinoprotein ethanol dehydrogenase from Pseudomonas aeruginosa ATCC 17933 crystallizes readily with the space group R3. The X-ray structure was solved at 2.6 A resolution by molecular replacement. Aside from differences in some loops, the folding of the enzyme is very similar to the larg...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3603

    authors： Keitel T,Diehl A,Knaute T,Stezowski JJ,Höhne W,Görisch H

    更新日期：2000-04-07 00:00:00

  • Structure-function analysis of the acyl carrier protein synthase (AcpS) from Mycobacterium tuberculosis.

    abstract：:We have solved the crystal structure of the acyl carrier protein synthase (AcpS) from Mycobacterium tuberculosis (Mtb) at 1.95 A resolution. AcpS, a 4-phosphopantetheinyl transferase, activates two distinct acyl carrier proteins (ACPs) that are present in fatty acid synthase (FAS) systems FAS-I and FAS-II, the ACP-I d...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.08.065

    authors： Dym O,Albeck S,Peleg Y,Schwarz A,Shakked Z,Burstein Y,Zimhony O

    更新日期：2009-11-06 00:00:00

  • Isolation of human antibody repertoires with preservation of the natural heavy and light chain pairing.

    abstract：:The humoral immune system in higher vertebrates is unique in its ability to generate highly diverse antibody responses against most pathogens as well as against certain malignancies. Several technologies have been developed to exploit this vast source of potentially therapeutic antibodies, including hybridoma technolo...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.02.040

    authors： Meijer PJ,Andersen PS,Haahr Hansen M,Steinaa L,Jensen A,Lantto J,Oleksiewicz MB,Tengbjerg K,Poulsen TR,Coljee VW,Bregenholt S,Haurum JS,Nielsen LS

    更新日期：2006-05-05 00:00:00

  • A genetic analysis of crystal growth.

    abstract：:The regulation of crystal morphology by proteins is often observed in biology. It is a central feature in the formation of hard tissues such as bones, teeth and mollusc shells. We have developed a genetic system in the bacterium Escherichia coli to study the protein-mediated control of crystal growth. We have used the...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3682

    authors： Brown S,Sarikaya M,Johnson E

    更新日期：2000-06-09 00:00:00

  • Differential stability of high-density lipoprotein subclasses: effects of particle size and protein composition.

    abstract：:High-density lipoproteins (HDLs) are complexes of proteins (mainly apoA-I and apoA-II) and lipids that remove cholesterol and prevent atherosclerosis. Understanding the distinct properties of the heterogeneous HDL population may aid the development of new diagnostic tools and therapies for atherosclerosis. Mature huma...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.02.036

    authors： Gao X,Yuan S,Jayaraman S,Gursky O

    更新日期：2009-04-03 00:00:00