Structurally constrained residues outside the binding motif are essential in the interaction of 14-3-3 and phosphorylated partner.

abstract：:Thorough knowledge of the molecular principles of protein-protein recognition is essential to our understanding of protein function at the cellular level. Whereas interactions of ordered proteins have been analyzed in great detail, complexes of intrinsically unstructured/disordered proteins (IUPs) have hardly been add...

journal_title：Journal of molecular biology

authors： Mészáros B,Tompa P,Simon I,Dosztányi Z

更新日期：2007-09-14 00:00:00

abstract：:Clathrin-coated vesicles mediate vesicular traffic in cells. Three-dimensional image reconstructions of homogenous populations of in vitro assembled clathrin coats have yielded a molecular model for clathrin and its interactions with some of its partners. The intrinsic averaging required for those calculations has pre...

journal_title：Journal of molecular biology

authors： Cheng Y,Boll W,Kirchhausen T,Harrison SC,Walz T

更新日期：2007-01-19 00:00:00

abstract：:SH3 domains are molecular-recognition modules that function by interacting with proteins containing sequences in polyproline II (PPII) conformation. The main limitation in designing short-ligand peptides to interact with these domains is the preservation of this helical arrangement, for which a high content of proline...

journal_title：Journal of molecular biology

authors： Cobos ES,Pisabarro MT,Vega MC,Lacroix E,Serrano L,Ruiz-Sanz J,Martinez JC

更新日期：2004-09-03 00:00:00

abstract：:The region of bacteriophage P1 DNA containing a lytic (vegetative) replicon has been identified by cloning P1 fragments into a phage lambda vector. We present the sequence of that replicon. Using a novel fusion vector containing two P1 loxP recombination sites, we have developed a transformation assay for replicon fun...

journal_title：Journal of molecular biology

authors： Sternberg N,Cohen G

更新日期：1989-05-05 00:00:00

abstract：:Eukaryotic cells contain three cytoskeletal filament systems that exhibit very distinct assembly properties, supramolecular architectures, dynamic behaviour and mechanical properties. Microtubules and microfilaments are relatively stiff polar structures whose assembly is modulated by the state of hydrolysis of the bou...

journal_title：Journal of molecular biology

authors： Mücke N,Kreplak L,Kirmse R,Wedig T,Herrmann H,Aebi U,Langowski J

更新日期：2004-01-30 00:00:00

abstract：:Pre-steady-state chemical quenched-flow techniques were used to study DNA unwinding catalyzed by Escherichia coli UvrD helicase (helicase II), a member of the SF1 helicase superfamily. Single turnover experiments, with respect to unwinding of a DNA oligonucleotide, were used to examine the DNA substrate and UvrD conce...

journal_title：Journal of molecular biology

authors： Ali JA,Maluf NK,Lohman TM

更新日期：1999-11-05 00:00:00

abstract：:Interactions of transmembrane helices play an important role in folding and oligomerization of integral membrane proteins. The interfacial residues of these helices frequently correspond to heptad repeat motifs. In order to uncover novel mechanisms underlying these interactions, we randomised a heptad repeat pattern w...

journal_title：Journal of molecular biology

authors： Ridder A,Skupjen P,Unterreitmeier S,Langosch D

更新日期：2005-12-09 00:00:00

abstract：:Alpha-actinin is the major F-actin crosslinking protein in both muscle and non-muscle cells. We report the crystal structure of the actin binding domain of human muscle alpha-actinin-3, which is formed by two consecutive calponin homology domains arranged in a "closed" conformation. Structural studies and available bi...

journal_title：Journal of molecular biology

authors： Franzot G,Sjöblom B,Gautel M,Djinović Carugo K

更新日期：2005-04-22 00:00:00

abstract：:Although numerous studies have been directed at understanding early folding events through the characterization of folding intermediates, there are few reports on the very late folding events, i.e. on the events taking place on the native side of the folding barrier and on alternative conformations of the folded state...

journal_title：Journal of molecular biology

authors： Otzen DE,Oliveberg M

更新日期：2002-02-01 00:00:00

abstract：:Mammalian Cdc25 phosphatase is responsible for the dephosphorylation of Cdc2 and other cyclin-dependent kinases at Thr14 and Tyr15, thus activating the kinase and allowing cell cycle progression. The catalytic domain of this dual-specificity phosphatase has recently been mapped to the 180 most C-terminal amino acids. ...

journal_title：Journal of molecular biology

authors： Hofmann K,Bucher P,Kajava AV

更新日期：1998-09-11 00:00:00

abstract：:In the present work, we have studied in vitro replication of N-2-acetylaminofluorene (AAF) or cis-diamminedichloroplatinum II (cis-DDP) single modified DNA templates. We used the holoenzyme (pol III HE) or the alpha subunit of DNA polymerase III, which is involved in SOS mutagenesis, and other DNA polymerases in order...

journal_title：Journal of molecular biology

authors： Belguise-Valladier P,Maki H,Sekiguchi M,Fuchs RP

更新日期：1994-02-11 00:00:00

abstract：:Mitochondrial respiratory chain complex I undergoes transitions from active to de-activated forms. We have investigated the phenomenon in sub-mitochondrial particles from Neurospora crassa wild-type and a null-mutant lacking the 29.9 kDa nuclear-coded subunit of complex I. Based on enzymatic activities, genetic crosse...

journal_title：Journal of molecular biology

authors： Ushakova AV,Duarte M,Vinogradov AD,Videira A

更新日期：2005-08-12 00:00:00

abstract：:We reported recently that activation of the inositol 1,4,5-triphosphate receptor (IP3R) is required for efficient HIV-1 Gag trafficking and viral particle release. IP3R activation requires phospholipase C (PLC)-catalyzed hydrolysis of PI(4,5)P(2) to IP3 and diacylglycerol. We show that Sprouty2 (Spry2), which binds PI...

journal_title：Journal of molecular biology

authors： Ehrlich LS,Medina GN,Carter CA

更新日期：2011-07-22 00:00:00

abstract：:The mathematical basis of the hypothesis that type-2 topoisomerases recognize and act at specific DNA juxtapositions has been investigated by coarse-grained lattice polymer models, showing that selective segment passages at hooked juxtapositions can result in dramatic reductions in catenane and knot populations. The l...

journal_title：Journal of molecular biology

authors： Liu Z,Zechiedrich L,Chan HS

更新日期：2010-07-30 00:00:00

abstract：:The histone N tails correspond to conserved amino acid sequences that are peripherally located in the nucleosome and undergo a variety of post-synthetic modifications during cell cycle. These N tails have been recently recognized as directly interacting with transcription-related proteins. We show here, based on circu...

journal_title：Journal of molecular biology

authors： Banères JL,Martin A,Parello J

更新日期：1997-10-31 00:00:00

abstract：:The crystal structure of the allosteric tetrameric hemoglobin from Scapharca inaequivalvis (HbII) has been determined in the carbonmonoxy liganded state using a combination of anomalous scattering and molecular replacement. The molecular model has been refined at 2.0 A resolution to a conventional R-factor of 0.173 an...

journal_title：Journal of molecular biology

authors： Royer WE Jr,Heard KS,Harrington DJ,Chiancone E

更新日期：1995-10-13 00:00:00

abstract：:We have previously described methodology that makes it possible to genetically encode a wide array of unnatural amino acids in both prokaryotic and eukaryotic organisms. Here, we report the systematic optimization of a Saccharomyces cerevisiae expression system for the production of mutant proteins containing unnatura...

journal_title：Journal of molecular biology

authors： Chen S,Schultz PG,Brock A

更新日期：2007-08-03 00:00:00

abstract：:Recent studies on iron-loaded transferrins have revealed a conformational change upon binding iron due to a domain closure. It has been suggested that the domain closure may be the key for the receptor recognition of the metal loaded transferrin (Grossmann et al., 1992). Small angle X-ray scattering has been used to p...

journal_title：Journal of molecular biology

authors： Grossmann JG,Neu M,Evans RW,Lindley PF,Appel H,Hasnain SS

更新日期：1993-02-05 00:00:00

abstract：:In bacteriophages of the cystovirus family, the polymerase complex (PX) encodes a 75-kDa RNA-directed RNA polymerase (P2) that transcribes the double-stranded RNA genome. Also a constituent of the PX is the essential protein P7 that, in addition to accelerating PX assembly and facilitating genome packaging, plays a re...

journal_title：Journal of molecular biology

authors： Alphonse S,Arnold JJ,Bhattacharya S,Wang H,Kloss B,Cameron CE,Ghose R

更新日期：2014-07-15 00:00:00

abstract：:Six mutants of Escherichia coli K12 that are sensitive to visible light have been isolated. Five of them, including an amber mutant, are defective in a gene that maps near 11 minutes on the linkage map of the chromosome, and this gene has been designated visA. The sixth mutant, which was isolated from bacteria that ca...

journal_title：Journal of molecular biology

authors： Miyamoto K,Nakahigashi K,Nishimura K,Inokuchi H

更新日期：1991-06-05 00:00:00

abstract：:Translating ribosomes often stall during elongation. The stalled ribosomes are known to be recycled by tmRNA (SsrA)-mediated trans-translation. Another process that recycles the stalled ribosomes is characterized by peptidyl-tRNA release. However, the mechanism of peptidyl-tRNA release from the stalled ribosomes is no...

journal_title：Journal of molecular biology

authors： Singh NS,Ahmad R,Sangeetha R,Varshney U

更新日期：2008-07-11 00:00:00

abstract：:Intercellular communication via gap junction membrane channels cannot occur until two apposing hemichannels (connexons) meet and dock to form a sealed cell-cell conduit. In particular, an important question is how does the structure at the extracellular surface influence the molecular recognition of the two connexons....

journal_title：Journal of molecular biology

authors： Perkins GA,Goodenough DA,Sosinsky GE

更新日期：1998-03-27 00:00:00

abstract：:DNA opening is an essential step in the initiation of replication via the Cairns mode of replication. The opening reaction was investigated in a gamma ori system by using hyperactive variants of plasmid R6K-encoded initiator protein, pi. Reactivity to KMnO4 (indicative of opening) within gamma ori DNA occurred in both...

journal_title：Journal of molecular biology

authors： Krüger R,Konieczny I,Filutowicz M

更新日期：2001-03-09 00:00:00

abstract：:The mitochondrial inner membrane consists of two morphologically distinct domains, the inner boundary membrane and large invaginations termed cristae. Narrow membrane structures, the crista junctions, link these two domains. Maintenance of this elaborate architecture depends on the evolutionarily conserved mitochondri...

journal_title：Journal of molecular biology

authors： Zerbes RM,Höß P,Pfanner N,van der Laan M,Bohnert M

更新日期：2016-04-24 00:00:00

abstract：:Using synthetic oligonucleotides, we have constructed a collection of Escherichia coli amber suppressor tRNA genes. In order to determine their specificities, these tRNAs were each used to suppress an amber (UAG) nonsense mutation in the E. coli dihydrofolate reductase gene fol. The mutant proteins were purified and s...

journal_title：Journal of molecular biology

authors： Normanly J,Kleina LG,Masson JM,Abelson J,Miller JH

更新日期：1990-06-20 00:00:00

abstract：:Actin polymerization plays a major role in cell movement. The controls of actin sequestration/desequestration and of filament turnover are two important features of cell motility. Actin binding proteins use properties derived from the steady-state monomer-polymer cycle of actin in the presence of ATP, to control the F...

journal_title：Journal of molecular biology

authors： Carlier MF,Pantaloni D

更新日期：1997-06-20 00:00:00

abstract：:We describe improvements of the currently most popular method for prediction of classically secreted proteins, SignalP. SignalP consists of two different predictors based on neural network and hidden Markov model algorithms, where both components have been updated. Motivated by the idea that the cleavage site position...

journal_title：Journal of molecular biology

authors： Bendtsen JD,Nielsen H,von Heijne G,Brunak S

更新日期：2004-07-16 00:00:00

abstract：:Molten globules are partially folded states of proteins which are generally believed to mimic structures formed during the folding process. In order to determine the minimal requirements for the formation of a molten globule state, we have prepared a set of peptide models of the molten globule state of human alpha-lac...

journal_title：Journal of molecular biology

authors： Demarest SJ,Boice JA,Fairman R,Raleigh DP

更新日期：1999-11-19 00:00:00

abstract：:Caspase recruitment domains (CARDs) are members of the death domain superfamily and contain six antiparallel helices in an alpha-helical Greek key topology. We have examined the equilibrium and kinetic folding of the CARD of Apaf-1 (apoptotic protease activating factor 1), which consists of 97 amino acid residues, at ...

journal_title：Journal of molecular biology

authors： Milam SL,Nicely NI,Feeney B,Mattos C,Clark AC

更新日期：2007-05-25 00:00:00

abstract：:The gene V protein of bacteriophage f1 binds to single-stranded nucleic acids and is essential for propagation of phage f1. We tested the function of gene V protein mutants with single amino acid substitutions in two ways: by the ability of the mutant proteins to support phage growth, and by the ability of the mutant ...

journal_title：Journal of molecular biology

authors： Terwilliger TC,Zabin HB,Horvath MP,Sandberg WS,Schlunk PM

更新日期：1994-02-18 00:00:00