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Cryo-electron tomography of clathrin-coated vesicles: structural implications for coat assembly.

Abstract：

:Clathrin-coated vesicles mediate vesicular traffic in cells. Three-dimensional image reconstructions of homogenous populations of in vitro assembled clathrin coats have yielded a molecular model for clathrin and its interactions with some of its partners. The intrinsic averaging required for those calculations has precluded detailed analysis of heterogeneous populations of clathrin-coated vesicles isolated from cells. We have therefore used cryo-electron tomography to study the lattice organization of individual clathrin-coated vesicles and the disposition of the captured vesicle with respect to the surrounding coat. We find a wide range of designs for the clathrin lattice, with different patterns of pentagonal, hexagonal, and occasionally heptagonal facets. Many coats, even smaller ones, enclose membrane vesicles, which are generally offset from the center of the clathrin shell. The electron density distribution between the coat and the underlying vesicle is not uniform, and the number of apparent contacts that anchor the clathrin lattice to the vesicle membrane is significantly less than the number of clathrin heavy chains in the assembly. We suggest that the eccentric position of the vesicle reflects the polarity of assembly, from initiation of coat formation to membrane pinching.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Cheng Y,Boll W,Kirchhausen T,Harrison SC,Walz T

doi

10.1016/j.jmb.2006.10.036

subject

Has Abstract

pub_date

2007-01-19 00:00:00

pages

892-9

issue

3

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(06)01414-8

journal_volume

365

pub_type

杂志文章

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