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Preliminary crystallographic studies of the Fab fragment of an anti-azophenylarsonate antibody.

Abstract：

:Single crystals of the Fab fragment of a murine A/J anti-azophenylarsonate monoclonal antibody have been prepared by the vapor diffusion method. Antibody 3A7 uses the same combination of variable region gene segments (VK, JK, VH, JH) as do anti-azophenylarsonate antibodies bearing a predominant cross-reactive idiotype, but utilizes a different D gene segment. The crystals grow in the presence of beta-octylglucoside as tetragonal bipyramids in the space group of either P4(1)2(1)2 or P4(3)3(1)2 and with unit cell dimensions of a = b = 77.9 A, and c = 146.7 A. They diffract X-rays to better than 2.7 A resolution. Data up to 2.7 A resolution have been collected.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Lin SF,Coll M,Gefter ML,Margolies MN,Wang AH

doi

10.1016/0022-2836(89)90587-1

subject

Has Abstract

pub_date

1989-04-20 00:00:00

pages

789-90

issue

4

eissn

0022-2836

issn

1089-8638

pii

0022-2836(89)90587-1

journal_volume

206

pub_type

杂志文章

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