Abstract：
:EmrE is a bacterial multidrug transporter of the small multidrug resistance family, which extrudes large hydrophobic cations such as tetraphenylphosphonium (TPP(+)) out of the cell by a proton antiport mechanism. Binding measurements were performed on purified EmrE solubilized in dodecylmaltoside to determine the stoichiometry of TPP(+) binding; the data showed that one TPP(+) molecule bound per EmrE dimer. Reconstitution of purified EmrE at low lipid:protein ratios in either the presence or the absence of TPP(+) produced well ordered two-dimensional crystals. Electron cryo-microscopy was used to collect images of frozen hydrated EmrE crystals and projection maps were determined by image processing to 7A resolution. An average native EmrE projection structure was calculated from the c222 and p222(1) crystals, which was subsequently subtracted from the average of two independent p2 projection maps of EmrE with TPP(+) bound. The interpretation of the difference density image most consistent with biochemical data suggested that TPP(+) bound at the monomer-monomer interface in the centre of the EmrE dimer, and resulted in the movement of at least one transmembrane alpha-helix.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Tate CG,Ubarretxena-Belandia I,Baldwin JMdoi
10.1016/s0022-2836(03)00895-7keywords:
subject
Has Abstractpub_date
2003-09-05 00:00:00pages
229-42issue
1eissn
0022-2836issn
1089-8638pii
S0022283603008957journal_volume
332pub_type
杂志文章abstract：:Anthracycline antibiotics daunomycin and adriamycin are among the most widely used in cancer chemotherapy and DNA is believed to be the primary target of their biological action. The crystal structure of a morpholino derivative of adriamycin bound to the DNA hexamer d(CGTACG) has been determined at 1.5 A resolution. T...
journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1993.1208
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1994.0144
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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journal_title：Journal of molecular biology
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/0022-2836(86)90438-9
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pub_type： 杂志文章
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：2013-01-23 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.2000.3844
更新日期：2000-07-07 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/j.jmb.2009.03.072
更新日期：2009-06-12 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/j.jmb.2005.11.012
更新日期：2006-02-10 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：2017-12-08 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/j.jmb.2004.01.060
更新日期：2004-03-26 00:00:00
abstract：:DNA methyltransferases flip their target base out of the DNA helix. Here, we have investigated base flipping by wild-type EcoRV DNA methyltransferase (M.EcoRV) and five M.EcoRV variants (D193A, Y196A, S229A, W231R and Y258A). These variants bind to DNA and S-adenosylmethionine but have a severely reduced catalytic eff...
journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1998.2389
更新日期：1999-01-22 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.2000.4405
更新日期：2001-02-23 00:00:00
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journal_title：Journal of molecular biology
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journal_title：Journal of molecular biology
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journal_title：Journal of molecular biology
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更新日期：1999-09-17 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1993-05-05 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：1988-11-05 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：1992-03-05 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：2007-02-23 00:00:00
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journal_title：Journal of molecular biology
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更新日期：1989-04-20 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：2007-11-02 00:00:00
abstract：:Long terminal repeat elements and retroviruses require primers for initiation of minus and plus-strand DNA synthesis by reverse transcriptase. Here we demonstrate genetically that plus-strand DNA synthesis of the yeast Ty1 element is initiated at two sites located at the 5' boundary of the 3' long terminal repeat (PPT...
journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1995.0553
更新日期：1995-10-20 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/s0022-2836(05)80154-8
更新日期：1995-04-07 00:00:00
