当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > NMR solution structure of Mob1, a mitotic exit network protein and its interaction with an NDR kinase peptide.

NMR solution structure of Mob1, a mitotic exit network protein and its interaction with an NDR kinase peptide.

Abstract：

:Proteins of the Mob1/phocein family are found in all eukaryotic cells. In yeast, they are activating subunits of Dbf2-related protein kinases involved in cell cycle control. Despite the wide occurrence of these proteins, their biological functions remain poorly understood. Here we report the solution structure of the Mob1 protein from Xenopus laevis solved by heteronuclear multidimensional NMR. The structure reveals a fold constituted by a central left-handed four-helix bundle, one connecting helix, two flanking helices and a long flexible loop. The clustering of two Cys and two His residues, and zinc measurement by atomic absorption spectroscopy support the existence of a zinc ion binding site. Our NMR structure is in good agreement with the recently described X-ray structure of human Mob1-A. Chemical shift perturbations observed upon addition of a peptide encompassing the basic region of the N-terminal regulatory domain of NDR kinase were used to identify and map a specific interaction between Mob1 and this kinase. The chemical shift changes indicate that the main interaction occurs on the acidic and conserved surface of Mob1. This surface was previously hypothesized to be the interaction surface according to the X-ray structure and was identified as functionally important in yeast. Our data suggest that the NDR kinase is a functional Dbf2 homologue in animal cells and contributes to the understanding of the molecular function of Mob1 proteins.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Ponchon L,Dumas C,Kajava AV,Fesquet D,Padilla A

doi

10.1016/j.jmb.2004.01.010

keywords:

subject

Has Abstract

pub_date

2004-03-12 00:00:00

pages

167-82

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022283604000592

journal_volume

337

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Structural assembly of the active site in an aldo-keto reductase by NADPH cofactor.

    abstract：:A 1.9 A resolution X-ray structure of the apo-form of Corynebacterium 2,5-diketo-d-gluconic acid reductase A (2,5-DKGR A), a member of the aldo-keto reductase superfamily, has been determined by molecular replacement using the NADPH-bound form of the same enzyme as the search model. 2,5-DKGR A catalyzes the NADPH-depe...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.4739

    authors： Sanli G,Blaber M

    更新日期：2001-06-22 00:00:00

  • Contribution of hydration to protein folding thermodynamics. I. The enthalpy of hydration.

    abstract：:The enthalpy of hydration of polar and non-polar groups upon protein unfolding has been estimated for four globular proteins in the temperature range 5 to 125 degrees C, using structural information on the groups in these proteins exposed to water in the native and unfolded states and volume-corrected calorimetric inf...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1416

    authors： Makhatadze GI,Privalov PL

    更新日期：1993-07-20 00:00:00

  • Crystallization and preliminary X-ray crystallographic studies of thermostable xylanase crystals isolated from Paecilomyces varioti.

    abstract：:A highly thermostable xylanase isolated from the thermophilic fungus Paecilomyces varioti has been crystallized by the vapour diffusion method. The isolation of this enzyme by crystallization directly from the culture filtrate projects this fungus as an important source for large-scale production of pure xylanase. The...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(94)90052-3

    authors： Eswaramoorthy S,Vithayathil PJ,Viswamitra MA

    更新日期：1994-11-04 00:00:00

  • Characterization of the interaction of GABARAPL-1 with the LIR motif of NBR1.

    abstract：:Selective autophagy requires the specific segregation of targeted proteins into autophagosomes. The selectivity is mediated by autophagy receptors, such as p62 and NBR1, which can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. Recogni...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2011.05.003

    authors： Rozenknop A,Rogov VV,Rogova NY,Löhr F,Güntert P,Dikic I,Dötsch V

    更新日期：2011-07-15 00:00:00

  • Conservation of orientation and sequence in protein domain--domain interactions.

    abstract：:The repertoire of naturally occurring protein structures is usually characterised in structural terms at the domain level by their constituent folds. As structure is acknowledged to be an important stepping stone to the understanding of protein function, an appreciation of how individual domain interactions are built ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.11.011

    authors： Littler SJ,Hubbard SJ

    更新日期：2005-02-04 00:00:00

  • The crystal structure of dihydrodipicolinate synthase from Escherichia coli at 2.5 A resolution.

    abstract：:The crystal structure of dihydrodipicolinate synthase from E. coli was determined by multiple isomorphous replacement methods. The structure was refined at a resolution of 2.5 A and the final R-factor is 19.6% for 32,190 reflections between 10.0 A and 2.5 A and F > 2 sigma (F). The crystallographic asymmetric unit con...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.0078

    authors： Mirwaldt C,Korndörfer I,Huber R

    更新日期：1995-02-10 00:00:00

  • Solution state NMR structure and dynamics of KpOmpA, a 210 residue transmembrane domain possessing a high potential for immunological applications.

    abstract：:The three-dimensional structure of the outer membrane protein A from Klebsiella pneumoniae transmembrane domain was determined by NMR.This protein induces specific humoral and cytotoxic responses, and is a potent carrier protein. This is one of the largest integral membrane proteins(210 residues) for which nearly comp...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.10.021

    authors： Renault M,Saurel O,Czaplicki J,Demange P,Gervais V,Löhr F,Réat V,Piotto M,Milon A

    更新日期：2009-01-09 00:00:00

  • Regulatory sequences in sigma 54 localise near the start of DNA melting.

    abstract：:Transcription initiation by the enhancer-dependent sigma(54) RNA polymerase holoenzyme is positively regulated after promoter binding. The promoter DNA melting process is subject to activation by an enhancer-bound activator protein with nucleoside triphosphate hydrolysis activity. Tethered iron chelate probes attached...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4393

    authors： Wigneshweraraj SR,Chaney MK,Ishihama A,Buck M

    更新日期：2001-03-02 00:00:00

  • Folding amphipathic helices into membranes: amphiphilicity trumps hydrophobicity.

    abstract：:High amphiphilicity is a hallmark of interfacial helices in membrane proteins and membrane-active peptides, such as toxins and antimicrobial peptides. Although there is general agreement that amphiphilicity is important for membrane-interface binding, an unanswered question is its importance relative to simple hydroph...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.05.016

    authors： Fernández-Vidal M,Jayasinghe S,Ladokhin AS,White SH

    更新日期：2007-07-13 00:00:00

  • RNase E and polyadenyl polymerase I are involved in maturation of CI RNA, the P4 phage immunity factor.

    abstract：:Bacteriophage P4 immunity is controlled by a small stable RNA (CI RNA) that derives from the processing of primary transcripts. In previous works, we observed that the endonuclease RNase P is required for the maturation of CI RNA 5'-end; moreover, we found that polynucleotide phosphorylase (PNPase), a 3' to 5' RNA-deg...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(02)00085-2

    authors： Briani F,Del Vecchio E,Migliorini D,Hajnsdorf E,Régnier P,Ghisotti D,Dehò G

    更新日期：2002-04-26 00:00:00

  • Crystal structures of penicillin-binding protein 3 (PBP3) from methicillin-resistant Staphylococcus aureus in the apo and cefotaxime-bound forms.

    abstract：:Staphylococcus aureus is a widespread Gram-positive opportunistic pathogen, and a methicillin-resistant form (MRSA) is particularly difficult to treat clinically. We have solved two crystal structures of penicillin-binding protein (PBP) 3 (PBP3) from MRSA, the apo form and a complex with the β-lactam antibiotic cefota...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.07.012

    authors： Yoshida H,Kawai F,Obayashi E,Akashi S,Roper DI,Tame JR,Park SY

    更新日期：2012-10-26 00:00:00

  • Recognition of protein substrates by the prolyl isomerase trigger factor is independent of proline residues.

    abstract：:The trigger factor is associated with bacterial ribosomes and catalyzes proline-limited protein folding reactions. Its folding activity is very high and conserved in evolution, as shown for the homologous enzymes from Escherichia coli and Mycoplasma genitalium. The folding protein substrate (a variant of ribonuclease ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1604

    authors： Scholz C,Mücke M,Rape M,Pecht A,Pahl A,Bang H,Schmid FX

    更新日期：1998-04-03 00:00:00

  • Shaping the Immune Landscape in Cancer by Galectin-Driven Regulatory Pathways.

    abstract：:Along with the discovery of tumor-driven inflammatory pathways, there has been a considerable progress over the past 10years in understanding the mechanisms leading to cancer immunosurveillance and immunoediting. Several regulatory pathways, typically involved in immune cell homeostasis, are co-opted by cancer cells t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2016.03.021

    authors： Rabinovich GA,Conejo-García JR

    更新日期：2016-08-14 00:00:00

  • prlF and yhaV encode a new toxin-antitoxin system in Escherichia coli.

    abstract：:Toxin-antitoxin systems consist of a stable toxin, frequently with endonuclease activity, and a small, labile antitoxin, which sequesters the toxin into an inactive complex. Under unfavorable conditions, the antitoxin is degraded, leading to activation of the toxin and resulting in growth arrest, possibly also in bact...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.07.016

    authors： Schmidt O,Schuenemann VJ,Hand NJ,Silhavy TJ,Martin J,Lupas AN,Djuranovic S

    更新日期：2007-09-28 00:00:00

  • Protein roles in group I intron RNA folding: the tyrosyl-tRNA synthetase CYT-18 stabilizes the native state relative to a long-lived misfolded structure without compromising folding kinetics.

    abstract：:The Neurospora crassa CYT-18 protein is a mitochondrial tyrosyl-tRNA synthetase that also promotes self-splicing of group I intron RNAs by stabilizing the functional structure in the conserved core. CYT-18 binds the core along the same surface as a common peripheral element, P5abc, suggesting that CYT-18 can replace P...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.11.009

    authors： Chadee AB,Bhaskaran H,Russell R

    更新日期：2010-01-22 00:00:00

  • The LcrG Tip Chaperone Protein of the Yersinia pestis Type III Secretion System Is Partially Folded.

    abstract：:The type III secretion system (T3SS) is essential in the pathogenesis of Yersinia pestis, the causative agent of plague. A small protein, LcrG, functions as a chaperone to the tip protein LcrV, and the LcrG-LcrV interaction is important in regulating protein secretion through the T3SS. The atomic structure of the LcrG...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2015.07.024

    authors： Chaudhury S,de Azevedo Souza C,Plano GV,De Guzman RN

    更新日期：2015-09-25 00:00:00

  • Exploring conformational space with a simple lattice model for protein structure.

    abstract：:We present a low resolution lattice model for which we can exhaustively generate all possible compact backbone conformations for small proteins. Using simple structural and energetic criteria, for a variety of proteins, we can select for lattice structures that have significant similarities with their known native str...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(94)90040-x

    authors： Hinds DA,Levitt M

    更新日期：1994-11-04 00:00:00

  • Secondary and tertiary structure formation of the beta-barrel membrane protein OmpA is synchronized and depends on membrane thickness.

    abstract：:The mechanism of membrane insertion and folding of a beta-barrel membrane protein has been studied using the outer membrane protein A (OmpA) as an example. OmpA forms an eight-stranded beta-barrel that functions as a structural protein and perhaps as an ion channel in the outer membrane of Escherichia coli. OmpA folds...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)01071-9

    authors： Kleinschmidt JH,Tamm LK

    更新日期：2002-11-22 00:00:00

  • Negative regulation of AAA + ATPase assembly by two component receiver domains: a transcription activation mechanism that is conserved in mesophilic and extremely hyperthermophilic bacteria.

    abstract：:Only a few transcriptional regulatory proteins have been characterized in extremely hyperthermophilic organisms, and most function as repressors. Structural features of the NtrC1 protein from the hyperthermophilic bacterium Aquifex aeolicus suggested that this protein functions similarly to the sigma(54)-polymerase ac...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.08.003

    authors： Doucleff M,Chen B,Maris AE,Wemmer DE,Kondrashkina E,Nixon BT

    更新日期：2005-10-21 00:00:00

  • Evidence that the KH RNA-binding domains influence the action of the E. coli NusA protein.

    abstract：:The NusA transcription elongation protein, which binds RNA, contains sequences corresponding to the S1 and KH classes of identified RNA binding domains. An essential function in E. coli, NusA is also one of the host factors required for action of the N transcription antitermination protein of lambda. Tandem KH domains...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00238-3

    authors： Zhou Y,Mah TF,Greenblatt J,Friedman DI

    更新日期：2002-05-17 00:00:00

  • Microscopy in Infectious Disease Research-Imaging Across Scales.

    abstract：:A comprehensive understanding of host-pathogen interactions requires quantitative assessment of molecular events across a wide range of spatiotemporal scales and organizational complexities. Due to recent technical developments, this is currently only achievable with microscopy. This article is providing a general per...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2018.06.018

    authors： Laketa V

    更新日期：2018-08-17 00:00:00

  • A generic mechanism of beta2-microglobulin amyloid assembly at neutral pH involving a specific proline switch.

    abstract：:Although numerous measurements of amyloid assembly of different proteins under distinct conditions in vitro have been performed, the molecular mechanisms underlying the specific self-association of proteins into amyloid fibrils remain obscure. Elucidating the nature of the events that initiate amyloid formation remain...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.01.013

    authors： Eichner T,Radford SE

    更新日期：2009-03-13 00:00:00

  • The mechanism of loading of the FLP recombinase onto its DNA target sequence.

    abstract：:The FLP recombinase interacts with its target sequence with the formation of three distinct DNA-protein complexes. The first complex leaves neither a DNase footprint nor is the DNA protected from methylation by dimethyl sulfate. We have found, however, that the FLP protein is bound predominantly to only one of the thr...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(88)90576-1

    authors： Beatty LG,Sadowski PD

    更新日期：1988-11-20 00:00:00

  • Infrared dichroism of twisted beta-sheet barrels. The structure of E. coli outer membrane proteins.

    abstract：:The infrared dichroic ratios of the amide bands from oriented beta-barrels yield an experimental value for the mean orientation, beta, of the beta-strands, relative to the barrel axis. For a barrel of n strands, this then gives the shear number, S, that characterizes the stagger of the beta-sheet. Combining values of ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3557

    authors： Marsh D

    更新日期：2000-03-31 00:00:00

  • An autonomously replicating sequence of pSRI plasmid is effective in two yeast species, Zygosaccharomyces rouxii and Saccharomyces cerevisiae.

    abstract：:The autonomously replicating sequences (ARSs) of pSR1, a cryptic circular DNA plasmid detected in a strain of Zygosaccharomyces rouxii, were delimited by subcloning and deletion analysis and by the isolation of nucleotide substitution mutations. A 30 base-pair (bp) sequence from inverted repeat 1 (IR1) and presumably ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(89)90242-8

    authors： Araki H,Oshima Y

    更新日期：1989-06-20 00:00:00

  • Transmembrane alpha-helix interactions are required for the functional assembly of the Escherichia coli Tol complex.

    abstract：:TolQ, TolR and TolA are membrane proteins involved in maintaining the structure of Escherichia coli cell envelope. TolQ and TolR span the inner membrane with three and with one alpha-helical segments, respectively. The tolQ925 mutation (A177V), located in the third putative transmembrane helix of TolQ (TolQ-III), indu...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.0058

    authors： Lazzaroni JC,Vianney A,Popot JL,Bénédetti H,Samatey F,Lazdunski C,Portalier R,Géli V

    更新日期：1995-02-10 00:00:00

  • Core RNA polymerase and promoter DNA interactions of purified domains of sigma N: bipartite functions.

    abstract：:The sigma N class of sigma factors confer upon RNA polymerase the requirement for enhancer-binding activator proteins. The sigma-N (sigma N) protein of Klebsiella pneumoniae was analysed by the assay of purified peptides comprising domains or regions of sigma N defined by proteolysis or by homology alignment, respecti...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0260

    authors： Cannon W,Missailidis S,Smith C,Cottier A,Austin S,Moore M,Buck M

    更新日期：1995-05-12 00:00:00

  • Modulation of substrate specificity of the DnaK chaperone by alteration of a hydrophobic arch.

    abstract：:Hsp70 chaperones assist protein folding by reversible interaction with extended hydrophobic segments of substrate polypeptides. We investigated the contribution of three structural elements of the substrate- binding cavity of the Escherichia coli homologue, DnaK, to substrate specificity by investigating mutant DnaK p...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4193

    authors： Rüdiger S,Mayer MP,Schneider-Mergener J,Bukau B

    更新日期：2000-12-01 00:00:00

  • Functional Antagonism of Human CD40 Achieved by Targeting a Unique Species-Specific Epitope.

    abstract：:Current clinical anti-CD40 biologic agents include both antagonist molecules for the treatment of autoimmune diseases and agonist molecules for immuno-oncology, yet the relationship between CD40 epitope and these opposing biological outcomes is not well defined. This report describes the identification of potent antag...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2016.05.014

    authors： Yamniuk AP,Suri A,Krystek SR,Tamura J,Ramamurthy V,Kuhn R,Carroll K,Fleener C,Ryseck R,Cheng L,An Y,Drew P,Grant S,Suchard SJ,Nadler SG,Bryson JW,Sheriff S

    更新日期：2016-07-17 00:00:00

  • Catabolite activator protein-induced DNA bending in transcription initiation.

    abstract：:We describe experiments that enable us to track the presence and direction of the DNA bend induced by Escherichia coli catabolite activator protein (CAP) through the intermediate stages of transcription initiation at the lac promoter. Transcriptional complexes examined were formed on superhelical templates to enhance ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90562-k

    authors： Zinkel SS,Crothers DM

    更新日期：1991-05-20 00:00:00