Translational activation in coliphage Qbeta: on a polycistronic messenger RNA, repression of one gene can activate translation of another.

abstract：:We have analysed the hydration of main-chain carbonyl and amide groups in 24 high-resolution well-refined protein structures as a function of the secondary structure in which these polar groups occur. We find that main-chain atoms in beta-sheets are as hydrated as those in alpha-helices, with most interactions involvi...

journal_title：Journal of molecular biology

authors： Thanki N,Umrania Y,Thornton JM,Goodfellow JM

更新日期：1991-09-20 00:00:00

abstract：:Certain pathogenic trypanosomatids are highly dependent on glycolysis for ATP production, and hence their glycolytic enzymes, including glycerol-3-phosphate dehydrogenase (GPDH), are considered attractive drug targets. The ternary complex structure of Leishmania mexicana GPDH (LmGPDH) with dihydroxyacetone phosphate (...

journal_title：Journal of molecular biology

authors： Choe J,Guerra D,Michels PA,Hol WG

更新日期：2003-05-30 00:00:00

abstract：:HBII-52 is a human brain-specific C/D box snoRNA that potentially regulates the editing and/or alternative splicing of the serotonin receptor. Forty-two nearly identical copies of the HBII-52 gene are located immediately downstream of the SNRPN protein-coding gene in an imprinted locus associated with Prader-Willi syn...

journal_title：Journal of molecular biology

authors： Nahkuri S,Taft RJ,Korbie DJ,Mattick JS

更新日期：2008-09-12 00:00:00

abstract：:The solution structure of r-elafin, a specific elastase inhibitor, has been determined using NMR spectroscopy. Characterized by a flat core and a flexible N-terminal extremity, the three-dimensional structure is formed by a central twisted beta-hairpin accompanied by two external segments linked by the proteinase bind...

journal_title：Journal of molecular biology

authors： Francart C,Dauchez M,Alix AJ,Lippens G

更新日期：1997-05-09 00:00:00

abstract：:Rabbit liver aldolase was purified by affinity elution on a CM52 ion exchanger. Crystals of rabbit liver aldolase suitable for X-ray diffraction experiments were grown from 45% saturated ammonium sulfate solution at 4 degrees C. The enzyme crystallizes in space group C222(1) having cell dimensions a = 377.02 A, b = 13...

journal_title：Journal of molecular biology

authors： Sygusch J,Beaudry D

更新日期：1985-11-05 00:00:00

abstract：:A maltodextrin-binding protein from Pyrococcus furiosus (PfuMBP) has been overproduced in Escherichia coli, purified, and crystallized. The crystal structure of the protein bound to an oligosaccharide ligand was determined to 1.85 A resolution. The fold of PfuMBP is very similar to that of the orthologous MBP from E. ...

journal_title：Journal of molecular biology

authors： Evdokimov AG,Anderson DE,Routzahn KM,Waugh DS

更新日期：2001-01-26 00:00:00

abstract：:This study examined the genomic organisation of the coding region of the glutathione S-transferase 3 (Ov-GST-3) from the human parasitic nematode Onchocerca volvulus; alternative splicing leads to three different transcripts (Ov-GST-3/1; Ov-GST-3/2 and Ov-GST-3/3). Since the expression of Ov-GST-3 is inducible by oxid...

journal_title：Journal of molecular biology

authors： Kampkötter A,Volkmann TE,de Castro SH,Leiers B,Klotz LO,Johnson TE,Link CD,Henkle-Dührsen K

更新日期：2003-01-03 00:00:00

abstract：:The Escherichia coli wild-type single strand binding (SSB) protein is a stable tetramer that binds to single-stranded (ss) DNA in its role in DNA replication, recombination and repair. The ssb-1 mutation, a substitution of tyrosine for histidine-55 within the SSB-1 protein, destabilizes the tetramer with respect to mo...

journal_title：Journal of molecular biology

authors： Bujalowski W,Lohman TM

更新日期：1991-01-05 00:00:00

abstract：:The group I intron from Tetrahymena thermophila is able to catalyze its own excision from a precursor RNA. The intron recognizes the splice sites through an intron-encoded sequence called the internal guide sequence, or IGS. The 5' and 3' exons are thought to align on the IGS and form a pseudoknot structure consisting...

journal_title：Journal of molecular biology

authors： Sargueil B,Tanner NK

更新日期：1993-10-20 00:00:00

abstract：:Rigid-body docking has become quite successful in predicting the correct conformations of binary protein complexes, at least when the constituent proteins do not undergo large conformational changes upon binding. However, determining whether two given proteins interact is a more difficult problem. Successful docking p...

journal_title：Journal of molecular biology

authors： Sacquin-Mora S,Carbone A,Lavery R

更新日期：2008-10-24 00:00:00

abstract：:The cellular prion protein (PrPC) comprises two domains: a globular C-terminal domain and an unstructured N-terminal domain. Recently, copper has been observed to drive tertiary contact in PrPC, inducing a neuroprotective cis interaction that structurally links the protein's two domains. The location of this interacti...

journal_title：Journal of molecular biology

authors： Schilling KM,Tao L,Wu B,Kiblen JTM,Ubilla-Rodriguez NC,Pushie MJ,Britt RD,Roseman GP,Harris DA,Millhauser GL

更新日期：2020-07-24 00:00:00

abstract：:Until now and based on the success of the helix/coil transition theory it has been assumed that the alpha-helical propensities of the amino acids are position independent. This has been critical to derive the set of theoretical parameters for the 20 natural amino acids. Here, we have analyzed the behavior of several n...

journal_title：Journal of molecular biology

authors： Petukhov M,Muñoz V,Yumoto N,Yoshikawa S,Serrano L

更新日期：1998-04-24 00:00:00

abstract：:The exceptional versatility of calmodulin (CaM) three-dimensional arrangement is reflected in the growing number of structural models of CaM/protein complexes currently available in the Protein Data Bank (PDB) database, revealing a great diversity of conformations, domain organization, and structural responses to Ca(2...

journal_title：Journal of molecular biology

authors： Villarroel A,Taglialatela M,Bernardo-Seisdedos G,Alaimo A,Agirre J,Alberdi A,Gomis-Perez C,Soldovieri MV,Ambrosino P,Malo C,Areso P

更新日期：2014-07-29 00:00:00

abstract：:LC3-associated phagocytosis (LAP) is a novel form of non-canonical autophagy where LC3 (microtubule-associated protein 1A/1B-light chain 3) is conjugated to phagosome membranes using a portion of the canonical autophagy machinery. The impact of LAP to immune regulation is best characterized in professional phagocytes,...

journal_title：Journal of molecular biology

authors： Heckmann BL,Boada-Romero E,Cunha LD,Magne J,Green DR

更新日期：2017-11-24 00:00:00

abstract：:By expressing a mutant trpR gene in an Escherichia coli strain that is trpR and has beta-galactosidase activity fused to the trp promoter/operator, thus putting the beta-galactosidase activity under the control of the Trp repressor, we can determine quantitatively the relative repression activity of such mutant(s). We...

journal_title：Journal of molecular biology

authors： Storbakk N,Fenton C,Riise HM,Nilsen IW,El-Gewely MR

更新日期：1996-03-15 00:00:00

abstract：:Gram-negative pathogens secrete effector proteins into human cells to modulate normal cellular processes and establish a bacterial replication niche. Shigella and pathogenic Escherichia coli possess homologous effector kinases, OspG and NleH1/2, respectively. Upon translocation, OspG but not NleH binds to ubiquitin an...

journal_title：Journal of molecular biology

authors： Grishin AM,Barber KR,Gu RX,Tieleman DP,Shaw GS,Cygler M

更新日期：2018-07-06 00:00:00

abstract：:A large conformational change is observed between HIV-1 proteinase in the ligand-free state and in complexes with transition-state inhibitors. Crystal structures of this enzyme have either the flaps open for the native or ligand-free enzyme or the flaps closed for peptidomimetic ligand-bound enzyme. We describe the st...

journal_title：Journal of molecular biology

authors： Wilderspin AF,Sugrue RJ

更新日期：1994-05-27 00:00:00

abstract：:Protein folding in the cell requires the assistance of enzymes collectively called chaperones. Among these, the chaperonins are 1-MDa ring-shaped oligomeric complexes that bind unfolded polypeptides and promote their folding within an isolated chamber in an ATP-dependent manner. Group II chaperonins, found in archaea ...

journal_title：Journal of molecular biology

authors： Lopez T,Dalton K,Frydman J

更新日期：2015-09-11 00:00:00

abstract：:Members of the Bcl-2 protein family regulate apoptosis by controlling the release of apoptogenic proteins such as cytochrome c from the mitochondrial intermembrane space. Proapoptotic members induce release by increasing outer membrane permeability, while antiapoptotic members prevent this. The activity of Bcl-2 prote...

journal_title：Journal of molecular biology

authors： Girard-Egrot A,Chauvet JP,Gillet G,Moradi-Améli M

更新日期：2004-01-02 00:00:00

abstract：:The recombination directionality factor, Xis, is a DNA bending protein that determines the outcome of integrase-mediated site-specific recombination by redesign of higher-order protein-DNA architectures. Although the attachment site DNA of mycobacteriophage Pukovnik is likely to contain four sites for Xis binding, Xis...

journal_title：Journal of molecular biology

authors： Singh S,Plaks JG,Homa NJ,Amrich CG,Héroux A,Hatfull GF,VanDemark AP

更新日期：2014-01-23 00:00:00

abstract：:The Escherichia coli trigger factor is a peptidyl-prolyl cis/trans isomerase (PPIase) which catalyzes proline-limited protein folding extremely well. It has been found associated with nascent protein chains as well as with the chaperone GroEL. The trigger factor utilizes protein regions outside the central catalytic d...

journal_title：Journal of molecular biology

authors： Zarnt T,Tradler T,Stoller G,Scholz C,Schmid FX,Fischer G

更新日期：1997-09-05 00:00:00

abstract：:Small, fast-growing bacteria make ideal subjects for genetic and quantitative analysis alike. Long the darling of theoreticians, efforts to understand the relationship between cell growth and cell cycle progression in bacterial systems have been propelled by modelers and empiricist in equal measure. Taking a historica...

journal_title：Journal of molecular biology

authors： Levin PA,Taheri-Araghi S

更新日期：2019-05-17 00:00:00

abstract：:The use of filamentous bacteriophage M13 as a vehicle for display of foreign peptides and proteins provides a means for the construction of therapeutic, diagnostic and technological tools of broad utility. The usefulness of this technology is dependent on the ability of an inserted peptide to act as a ligand when fuse...

journal_title：Journal of molecular biology

authors： Kishchenko G,Batliwala H,Makowski L

更新日期：1994-08-12 00:00:00

abstract：:Residue-specific potentials between pairs of side-chains and pairs of side-chain-backbone interaction sites have been generated by collecting radial distribution data for 302 protein structures. Multiple atomic interactions have been utilized to enhance the specificity and smooth the distance-dependence of the potenti...

journal_title：Journal of molecular biology

authors： Bahar I,Jernigan RL

更新日期：1997-02-14 00:00:00

abstract：:Infection of Escherichia coli by filamentous bacteriophages is mediated by the minor phage coat protein g3p and involves two distinct cellular receptors, the F' pilus and the periplasmic protein TolA. Recently we have shown that the two receptors are contacted in a sequential manner, such that binding of TolA by the N...

journal_title：Journal of molecular biology

authors： Holliger P,Riechmann L,Williams RL

更新日期：1999-05-14 00:00:00

abstract：:Regulation of eukaryotic genes is largely governed by multiple cis-acting DNA sequences recognized by specific transcription factors. The transcription factor NF-kappa B has been implicated as an important regulator of cellular and viral genes, including those of immunoglobulin kappa light chain, interleukin-2, beta-i...

journal_title：Journal of molecular biology

authors： Pettersson M,Schaffner W

更新日期：1990-07-20 00:00:00

abstract：:Human alpha-galactosidase A (alpha-D-galactoside galactohydrolase; EC 3.2.1.22), the glycosylated lysosomal enzyme deficient in Fabry disease, has been crystallized as a complex with the inhibitor N-6-aminohexanoyl-alpha-D-galactopyranosylamine. The "hanging drop" method of vapor diffusion was used to grow crystals fr...

journal_title：Journal of molecular biology

authors： Murali R,Ioannou YA,Desnick RJ,Burnett RM

更新日期：1994-06-17 00:00:00

abstract：:The three-dimensional structure of L-fucose isomerase from Escherichia coli has been determined by X-ray crystallography at 2.5 A resolution. This ketol isomerase converts the aldose L-fucose into the corresponding ketose L-fuculose using Mn2+ as a cofactor. Being a hexamer with 64,976 Da per subunit, L-fucose isomera...

journal_title：Journal of molecular biology

authors： Seemann JE,Schulz GE

更新日期：1997-10-17 00:00:00

abstract：:Shikimate kinase (SK) and other enzymes in the shikimate pathway are potential targets for developing non-toxic antimicrobial agents, herbicides, and anti-parasite drugs, because the pathway is essential in the above species but is absent from mammals. The crystal structure of Mycobacterium tuberculosis SK (MtSK) in c...

journal_title：Journal of molecular biology

authors： Gu Y,Reshetnikova L,Li Y,Wu Y,Yan H,Singh S,Ji X

更新日期：2002-06-07 00:00:00

abstract：:The stability and (un)folding of the 19-residue peptide, SCVTLYQSWRYSQADNGCA, corresponding to the first beta-hairpin (residues 10 to 28) of the alpha-amylase inhibitor tendamistat (PDB entry 3AIT) has been studied by molecular dynamics simulations in explicit water under periodic boundary conditions at several temper...

journal_title：Journal of molecular biology

authors： Bonvin AM,van Gunsteren WF

更新日期：2000-02-11 00:00:00