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Folding of RNA involves parallel pathways.

Abstract：

:Folding kinetics of large RNAs are just beginning to be investigated. We show that the Tetrahymena self-splicing RNA partitions into a population that rapidly reaches the native state, and a slowly folding population that is trapped in metastable misfolded structures. Transitions from the misfolded structures to the native state involve partial unfolding. The total yield of native RNA is increased by iterative annealing of the inactive population, and mildly denaturing conditions increase the rate of folding at physiological temperatures. These results provide the first evidence that an RNA can fold by multiple parallel paths.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Pan J,Thirumalai D,Woodson SA

doi

10.1006/jmbi.1997.1311

subject

Has Abstract

pub_date

1997-10-17 00:00:00

pages

7-13

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(97)91311-5

journal_volume

273

pub_type

杂志文章

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