Crystallization of halophilic malate dehydrogenase from Halobacterium marismortui.

abstract：:The eukaryotic 26S proteasome is a large multisubunit complex that degrades the majority of proteins in the cell under normal conditions. The 26S proteasome can be divided into two subcomplexes: the 19S regulatory particle and the 20S core particle. Most substrates are first covalently modified by ubiquitin, which the...

journal_title：Journal of molecular biology

authors： Budenholzer L,Cheng CL,Li Y,Hochstrasser M

更新日期：2017-11-10 00:00:00

abstract：:Switching between the active (ATP and DNA bound) and inactive conformations of the homologous recombination RecA protein is regulated by ATP hydrolysis. First, we use the homologous pairing domain of RecA derived from its mobile loop L2 to show that the interaction of this random coil peptide with the gamma-phosphate ...

journal_title：Journal of molecular biology

authors： Voloshin ON,Wang L,Camerini-Otero RD

更新日期：2000-11-10 00:00:00

abstract：:We have isolated and sequenced a cloned complementary DNA insert complementary to the messenger RNA of a cytoplasmic actin expressed in human epidermal cells. This provides the first cytoplasmic actin complementary DNA sequence for a vertebrate organism. The actin amino acid sequence predicted from this complementary ...

journal_title：Journal of molecular biology

authors： Hanukoglu I,Tanese N,Fuchs E

更新日期：1983-02-05 00:00:00

abstract：:Single crystals of the soluble glucose dehydrogenase (GDH) from Acinetobacter calcoaceticus have been grown by the vapour diffusion method. These crystals diffract to beyond 2.1 A and are suitable for X-ray crystallography. The space group was determined to be P2(1) with unit cell parameters a = 55.5 A, b = 104.5 A, c...

journal_title：Journal of molecular biology

authors： Schlunegger MP,Grütter MG,Streiff MB,Olsthoorn AJ,Duine JA

更新日期：1993-10-20 00:00:00

abstract：:We have developed a self-assembly system for nucleosome arrays in which recombinant, post-translationally unmodified histone proteins are combined with DNA of defined-sequence to form chromatin higher-order structure. The nucleosome arrays obtained are highly homogeneous and sediment at 53S when maximally folded in 1m...

journal_title：Journal of molecular biology

authors： Dorigo B,Schalch T,Bystricky K,Richmond TJ

更新日期：2003-03-14 00:00:00

abstract：:Tat (trans-activator) proteins are early RNA binding proteins regulating lentiviral transcription. These proteins are necessary components in the life cycle of all known lentiviruses, such as the human immunodeficiency viruses (HIV) or the equine infectious anemia virus (EIAV). Tat proteins are thus ideal targets for ...

journal_title：Journal of molecular biology

authors： Bayer P,Kraft M,Ejchart A,Westendorp M,Frank R,Rösch P

更新日期：1995-04-07 00:00:00

abstract：:A new statistic Sc, which has a number of advantages over other measures of packing, is used to examine the shape complementarity of protein/protein interfaces selected from the Brookhaven Protein Data Bank. It is shown using Sc that antibody/antigen interfaces as a whole exhibit poorer shape complementarity than is o...

journal_title：Journal of molecular biology

authors： Lawrence MC,Colman PM

更新日期：1993-12-20 00:00:00

abstract：:Induction of gene expression has great potential in the treatment of many human diseases. Peptide nucleic acid (PNA) as a novel DNA-binding reagent provides an ideal system to induce gene-specific expression. In our recent studies, we have demonstrated that PNA bound to double-stranded DNA targets and, therefore, gene...

journal_title：Journal of molecular biology

authors： Wang G,Jing K,Balczon R,Xu X

更新日期：2001-11-09 00:00:00

abstract：:Plasmids were constructed in which a HindIII fragment of rDNA (6.4 x 10(3) base-pairs) was inserted into vectors pGEM-1 and 2 in both orientations. The DNA insert encoded the yeast 35 S precursor rRNA beginning 180 bases upstream from the 5' end of the mature 18 S rRNA and ending 289 bases beyond the 3' end of the mat...

journal_title：Journal of molecular biology

authors： Thweatt R,Lee JC

更新日期：1990-01-20 00:00:00

abstract：:The cellulosome is a highly elaborate cell-bound multienzyme complex that efficiently orchestrates the deconstruction of cellulose and hemicellulose, two of the nature's most abundant polymers. Understanding the intricacy of these nanomachines evolved by anaerobic microbes could sustain the development of an effective...

journal_title：Journal of molecular biology

authors： García-Alvarez B,Melero R,Dias FM,Prates JA,Fontes CM,Smith SP,Romão MJ,Carvalho AL,Llorca O

更新日期：2011-04-08 00:00:00

abstract：:To study mechanisms of nucleosome positioning, small circular plasmids were constructed, assembled into chromatin in vivo in Saccharomyces cerevisiae, and their chromatin structures were analysed with respect to positions of nucleosomes and nuclease-sensitive regions. Plasmids used include insertions of the URA3 gene ...

journal_title：Journal of molecular biology

authors： Thoma F

更新日期：1986-07-20 00:00:00

abstract：:KorA and KorB proteins of IncP1 plasmid RK2 are encoded in the central control region (ccr) of the plasmid and act as global regulators of plasmid genes for replication, transfer and stable inheritance. KorA represses seven promoters on RK2, by binding to a defined operator site, OA, which always occurs in promoter re...

journal_title：Journal of molecular biology

authors： Kostelidou K,Jones AC,Thomas CM

更新日期：1999-06-04 00:00:00

abstract：:An analogue of the important folding intermediate of BPTI with only the disulphide bond between Cys30 and Cys51 has been characterized by 1H and 15N NMR techniques. In particular, the dynamics of the polypeptide backbone were characterized using (1H)-15N NOE and 15N T1 and T2 relaxation data. The intermediate is parti...

journal_title：Journal of molecular biology

authors： van Mierlo CP,Darby NJ,Keeler J,Neuhaus D,Creighton TE

更新日期：1993-02-20 00:00:00

abstract：:The folding thermodynamics and kinetics of the alpha-spectrin SH3 domain with a redesigned hydrophobic core have been studied. The introduction of five replacements, A11V, V23L, M25V, V44I and V58L, resulted in an increase of 16% in the overall volume of the side-chains forming the hydrophobic core but caused no remar...

journal_title：Journal of molecular biology

authors： Cobos ES,Filimonov VV,Vega MC,Mateo PL,Serrano L,Martínez JC

更新日期：2003-04-18 00:00:00

abstract：:This study describes an algorithm that finds rho-independent transcription terminators in bacterial genomes and evaluates the accuracy of its predictions. The algorithm identifies terminators by searching for a common mRNA motif: a hairpin structure followed by a short uracil-rich region. For each terminator, an energ...

journal_title：Journal of molecular biology

authors： Ermolaeva MD,Khalak HG,White O,Smith HO,Salzberg SL

更新日期：2000-08-04 00:00:00

abstract：:We have previously described the organization of a 13.3 kb region of the Aspergillus nidulans genome, designated SpoC1, coding for multiple poly(A)+ RNAs that accumulate in asexual spores but not in somatic cells. We have determined the limits of the SpoC1 gene cluster by investigating the transcriptional features of ...

journal_title：Journal of molecular biology

authors： Gwynne DI,Miller BL,Miller KY,Timberlake WE

更新日期：1984-11-25 00:00:00

abstract：:Zinc-induced tubulin sheets without microtubule-associated proteins (MAPs) were assembled from tubulin purified by phosphocellulose chromatography. Large, open sheets were obtained in five-minute incubations at pH 5.7. Electron micrographs of negatively stained sheets showed a protofilament arrangement similar to that...

journal_title：Journal of molecular biology

authors： McEwen BF,Ceska TA,Crepeau RH,Edelstein SJ

更新日期：1983-05-15 00:00:00

abstract：:The thermodynamic hypothesis of Anfinsen postulates that structures and stabilities of globular proteins are determined by their amino acid sequences. Chain topology, however, is known to influence the folding reaction, in that motifs with a preponderance of local interactions typically fold more rapidly than those wi...

journal_title：Journal of molecular biology

authors： Hills RD Jr,Kathuria SV,Wallace LA,Day IJ,Brooks CL 3rd,Matthews CR

更新日期：2010-04-30 00:00:00

abstract：:The three-dimensional structure of [Cd7]-metallothionein-2 from rat liver was determined in aqueous solution, using nuclear magnetic resonance spectrometry and distance geometry calculations. The experimental data provided proton-proton distance constraints from measurements of nuclear Overhauser effects, constraints ...

journal_title：Journal of molecular biology

authors： Schultze P,Wörgötter E,Braun W,Wagner G,Vasák M,Kägi JH,Wüthrich K

更新日期：1988-09-05 00:00:00

abstract：:The emergence and spread of multi-drug-resistant strains of Staphylococcus aureus in hospitals and in the community emphasize the urgency for the development of novel therapeutic interventions. Our approach was to evaluate the potential of harnessing the human immune system to guide the development of novel therapeuti...

journal_title：Journal of molecular biology

authors： Foletti D,Strop P,Shaughnessy L,Hasa-Moreno A,Casas MG,Russell M,Bee C,Wu S,Pham A,Zeng Z,Pons J,Rajpal A,Shelton D

更新日期：2013-05-27 00:00:00

abstract：:We have measured the solubility, and the rates of homogeneous and heterogeneous nucleation on sickle hemoglobin (HbS beta 6 Glu-->Val) additionally modified by site-directed mutagenesis to possess Ala rather than Leu at beta 88, which forms part of the receptor site for beta 6 Val in the sickle polymer. The solubility...

journal_title：Journal of molecular biology

authors： Cao Z,Liao D,Mirchev R,Martin de Llano JJ,Himanen JP,Manning JM,Ferrone FA

更新日期：1997-02-07 00:00:00

abstract：:In both prokaryotic and eukaryotic organisms, repressors and activators are responsible for regulating gene expression. The lac operon is a paradigm for understanding how metabolites function as signaling molecules and modulate transcription. These metabolites or allosteric effector molecules bind to the repressor and...

journal_title：Journal of molecular biology

authors： Daber R,Sharp K,Lewis M

更新日期：2009-10-09 00:00:00

abstract：:The fragment crystallizable (Fc) region links the key pathogen identification and destruction properties of immunoglobulin G (IgG). Pathogen opsonization positions Fcs to activate pro-inflammatory Fcγ receptors (FcγRs) on immune cells. The cellular response and committal to a damaging, though protective, immune respon...

journal_title：Journal of molecular biology

authors： Frank M,Walker RC,Lanzilotta WN,Prestegard JH,Barb AW

更新日期：2014-04-17 00:00:00

abstract：:We describe improvements of the currently most popular method for prediction of classically secreted proteins, SignalP. SignalP consists of two different predictors based on neural network and hidden Markov model algorithms, where both components have been updated. Motivated by the idea that the cleavage site position...

journal_title：Journal of molecular biology

authors： Bendtsen JD,Nielsen H,von Heijne G,Brunak S

更新日期：2004-07-16 00:00:00

abstract：:Motifs N2 and N3, also referred to as switch-1 and switch-2, form part of the active site of molecular motors such as myosins and kinesins. In the case of myosin, N3 is thought to act as a gamma-phosphate sensor and moves almost 6 A relative to N2 during the catalysed turnover of ATP, opening and closing the active si...

journal_title：Journal of molecular biology

authors： Furch M,Fujita-Becker S,Geeves MA,Holmes KC,Manstein DJ

更新日期：1999-07-16 00:00:00

abstract：:A family of Structural Maintenance of Chromosome (SMC) complexes is essential for key cellular processes ensuring proper cohesion, condensation and replication. They share a common SMC-kleisin architecture allowing them to embrace DNA. In SMC5/6, the NSE1 and NSE3 KITE and NSE4 kleisin subunits form a stable subcomple...

journal_title：Journal of molecular biology

authors： Adamus M,Lelkes E,Potesil D,Ganji SR,Kolesar P,Zabrady K,Zdrahal Z,Palecek JJ

更新日期：2020-06-12 00:00:00

abstract：:RT6 is a glycosyl phosphatidylinositol-anchored cell membrane protein, whose expression is restricted to peripheral T cells and intraepithelial lymphocytes. It has attracted interest as a T cell differentiation marker and activation antigen in rats. The only known protein to which RT6 shows significant homology is a r...

journal_title：Journal of molecular biology

authors： Haag F,Koch-Nolte F,Kühl M,Lorenzen S,Thiele HG

更新日期：1994-10-28 00:00:00

abstract：:Suppressor of cytokine signalling 3 (SOCS3) is responsible for regulating the cellular response to a variety of cytokines, including interleukin 6 and leukaemia inhibitory factor. Identification of the SOCS box domain led to the hypothesis that SOCS3 can associate with functional E3 ubiquitin ligases and thereby induc...

journal_title：Journal of molecular biology

authors： Babon JJ,Sabo JK,Soetopo A,Yao S,Bailey MF,Zhang JG,Nicola NA,Norton RS

更新日期：2008-09-12 00:00:00

abstract：:Mammalian lipoxygenases form a heterogeneous family of lipid peroxidizing enzymes, which have been implicated in synthesis of inflammatory mediators, in cell development and in the pathogenesis of various diseases (atherosclerosis, osteoporosis) with major health political importance. The crystal structures of two pla...

journal_title：Journal of molecular biology

authors： Hammel M,Walther M,Prassl R,Kuhn H

更新日期：2004-10-29 00:00:00

abstract：:The powerful explanatory paradigm of molecular biology requiring form to co-evolve with function has again been proven successful when, over the recent two decades, a wealth of biological functions have been uncovered for RNA. Previously considered as a mere mediator of the genetic code, RNA is now acknowledged as a k...

journal_title：Journal of molecular biology

authors： Hermann T,Patel DJ

更新日期：1999-12-10 00:00:00