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Mutations upstream of the ribosome-binding site affect translational efficiency.

Abstract：

:The DNA coding for the major outer membrane lipoprotein of Escherichia coli has been fused to the coding region of the beta-galactosidase gene to measure the effect of various mutations on the efficiency of translation initiation. The various mutants were made by either inserting or deleting a small number of nucleotides into or from a region just upstream of the ribosome-binding site. These small mutations dramatically affect translation initiation as measured by the production of beta-galactosidase. We postulate that these mutations affect translation initiation by altering the secondary structure of the messenger RNA. In one case, we predict that a stem and loop just upstream of the Shine-Dalgarno sequence sterically hinders the binding of the ribosome to the mRNA.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Coleman J,Inouye M,Nakamura K

doi

10.1016/0022-2836(85)90332-8

subject

Has Abstract

pub_date

1985-01-05 00:00:00

pages

139-43

issue

1

eissn

0022-2836

issn

1089-8638

pii

0022-2836(85)90332-8

journal_volume

181

pub_type

杂志文章

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