Abstract:
:Human granulocyte colony-stimulating factor (hG-CSF) specifically stimulates proliferation of neutrophils. Two crystal forms of a mutant of hG-CSF expressed in Escherichia coli have been obtained using the hanging drop vapour diffusion method. One form is triclinic, space group P1, with cell dimensions a = 37.3 A, b = 46.4 A, c = 47.7 A, alpha = 105.5 degrees, beta = 98.0 degrees and gamma = 109.4 degrees. The other is monoclinic, space group C2, with cell dimensions a = 82.0 A, b = 49.2 A, c = 49.4 A and beta = 113.9 degrees. Both crystal forms diffract beyond 2.0 A and are suitable for X-ray analysis.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Nagahara Y,Konishi N,Yokoo Y,Hirayama Ndoi
10.1016/0022-2836(90)90143-Asubject
Has Abstractpub_date
1990-07-05 00:00:00pages
25-6issue
1eissn
0022-2836issn
1089-8638pii
0022-2836(90)90143-Ajournal_volume
214pub_type
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