Crystallization and preliminary diffraction studies of recombinant human granulocyte-stimulating factor (KW2228).

Abstract:

:Human granulocyte colony-stimulating factor (hG-CSF) specifically stimulates proliferation of neutrophils. Two crystal forms of a mutant of hG-CSF expressed in Escherichia coli have been obtained using the hanging drop vapour diffusion method. One form is triclinic, space group P1, with cell dimensions a = 37.3 A, b = 46.4 A, c = 47.7 A, alpha = 105.5 degrees, beta = 98.0 degrees and gamma = 109.4 degrees. The other is monoclinic, space group C2, with cell dimensions a = 82.0 A, b = 49.2 A, c = 49.4 A and beta = 113.9 degrees. Both crystal forms diffract beyond 2.0 A and are suitable for X-ray analysis.

journal_name

J Mol Biol

authors

Nagahara Y,Konishi N,Yokoo Y,Hirayama N

doi

10.1016/0022-2836(90)90143-A

subject

Has Abstract

pub_date

1990-07-05 00:00:00

pages

25-6

issue

1

eissn

0022-2836

issn

1089-8638

pii

0022-2836(90)90143-A

journal_volume

214

pub_type

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