Abstract:
:The discovery that RNA molecules can fold into complex structures and carry out diverse cellular roles has led to interest in developing tools for modeling RNA tertiary structure. While significant progress has been made in establishing that the RNA backbone is rotameric, few libraries of discrete conformations specifically for use in RNA modeling have been validated. Here, we present six libraries of discrete RNA conformations based on a simplified pseudo-torsional notation of the RNA backbone, comparable to phi and psi in the protein backbone. We evaluate the ability of each library to represent single nucleotide backbone conformations, and we show how individual library fragments can be assembled into dinucleotides that are consistent with established RNA backbone descriptors spanning from sugar to sugar. We then use each library to build all-atom models of 20 test folds, and we show how the composition of a fragment library can limit model quality. Despite the limitations inherent in using discretized libraries, we find that several hundred discrete fragments can rebuild RNA folds up to 174 nucleotides in length with atomic-level accuracy (<1.5 Å RMSD). We anticipate that the libraries presented here could easily be incorporated into RNA structural modeling, analysis, or refinement tools.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Humphris-Narayanan E,Pyle AMdoi
10.1016/j.jmb.2012.03.002subject
Has Abstractpub_date
2012-08-03 00:00:00pages
6-26issue
1eissn
0022-2836issn
1089-8638pii
S0022-2836(12)00240-9journal_volume
421pub_type
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