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Structural alteration in alternating adenine-thymine sequences in positively supercoiled DNA.

Abstract：

:An alternating adenine-thymine tract in a relaxed closed circular plasmid was found to become strongly reactive to osmium tetroxide in the presence of actinomycin D. We suggest that this is due to a local overwinding of the alternating tract as a result of positive supercoiling induced by intercalation of the antibiotic at GpC sequences elsewhere in the DNA. We have previously shown that (A.T)n sequences undergo a local underwinding in response to negative supercoiling, and it appears that such sequences are especially torsionally deformable in both directions.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

McClellan JA,Lilley DM

doi

10.1016/0022-2836(91)90555-k

subject

Has Abstract

pub_date

1991-05-20 00:00:00

pages

145-9

issue

2

eissn

0022-2836

issn

1089-8638

pii

0022-2836(91)90555-K

journal_volume

219

pub_type

杂志文章

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