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RNA-protein organization of U1, U5 and U4-U6 small nuclear ribonucleoproteins in HeLa cells.

Abstract：

:Small nuclear ribonucleoproteins (snRNPs) containing U1 and U5 snRNAs from HeLa cells have been fractionated using a combination of isopycnic centrifugation in cesium chloride and ion-exchange chromatography on DEAE-Sepharose. The procedure is based on the extreme stability conferred upon snRNPs by Mg2+ enabling them to withstand the very high ionic strength that prevails in cesium chloride. U1 snRNP prepared by this method contains all nine major proteins (68K, A, B, B', C, D, E, F, G) corresponding to those previously identified by immunoprecipitation and is therefore precipitable by anti-RNP and anti-Sm antibodies. U5 snRNP purified in this way contains the common D to G proteins and is also enriched in a 25 X 10(3) Mr protein that may be U5 snRNP-specific. The core-resistant U5 snRNA sequence (nucleotide 84 to 3' OH) covered by D to G proteins is extended by only six nucleotides. A similar situation is seen in U4-U6 snRNP, which we have obtained in a sufficiently pure form to examine protected sequences. However, the core-resistant sequence of U4 (nucleotide 116 to 3' OH) in U4-U6 snRNP is extended by 37 nucleotides, suggesting that the protein composition of this particle could be more complex than that of U5 snRNP. The ribonucleoprotein organization of snRNPs is summarized and discussed in view of our current knowledge on snRNA sequences protected by proteins.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Lelay-Taha MN,Reveillaud I,Sri-Widada J,Brunel C,Jeanteur P

doi

10.1016/0022-2836(86)90321-9

subject

Has Abstract

pub_date

1986-06-05 00:00:00

pages

519-32

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(86)90321-9

journal_volume

189

pub_type

杂志文章

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