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The 29.9 kDa subunit of mitochondrial complex I is involved in the enzyme active/de-active transitions.

Abstract：

:Mitochondrial respiratory chain complex I undergoes transitions from active to de-activated forms. We have investigated the phenomenon in sub-mitochondrial particles from Neurospora crassa wild-type and a null-mutant lacking the 29.9 kDa nuclear-coded subunit of complex I. Based on enzymatic activities, genetic crosses and analysis of mitochondrial proteins in sucrose gradients, we found that about one-fifth of complex I with catalytic properties similar to the wild-type enzyme is assembled in the mutant. Mutant complex I still displays active/de-active transitions, indicating that other proteins are involved in the phenomenon. However, the kinetic characteristics of complex I active/de-active transitions in nuo29.9 differ from wild-type. The spontaneous de-activation of the mutant enzyme is much slower, implicating the 29.9 kDa polypeptide in this event. We suggest that the fungal 29.9 kDa protein and its homologues in other organisms may modulate the active/de-active transitions of complex I.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Ushakova AV,Duarte M,Vinogradov AD,Videira A

doi

10.1016/j.jmb.2005.06.005

keywords:

subject

Has Abstract

pub_date

2005-08-12 00:00:00

pages

327-33

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(05)00651-0

journal_volume

351

pub_type

杂志文章

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