Efficient utilization of Escherichia coli transcriptional signals in Bacillus subtilis.

abstract：:Antibodies and prorenin mutants have long been used to structurally characterize prorenin, the inactive proenzyme form of renin. They were designed on the basis of homology models built using other aspartyl protease proenzyme structures since no structure was available for prorenin. Here, we present the first X-ray st...

journal_title：Journal of molecular biology

authors： Morales R,Watier Y,Böcskei Z

更新日期：2012-08-03 00:00:00

abstract：:Bacterial microcompartments (BMCs) sequester enzymes from the cytoplasmic environment by encapsulation inside a selectively permeable protein shell. Bioinformatic analyses indicate that many bacteria encode BMC clusters of unknown function and with diverse combinations of shell proteins. The genome of the halophilic m...

journal_title：Journal of molecular biology

authors： Lassila JK,Bernstein SL,Kinney JN,Axen SD,Kerfeld CA

更新日期：2014-05-29 00:00:00

abstract：:Arc repressor from bacteriophage P22 is a homodimeric member of the ribbon-helix-helix family of transcription factors. Two dimers bind cooperatively to adjacent sites on operator DNA with the antiparallel beta-sheet of each Arc dimer contacting the major groove. Despite the 2-fold symmetry of the dimer, the sequence ...

journal_title：Journal of molecular biology

authors： Spector S,Sauer RT,Tidor B

更新日期：2004-07-02 00:00:00

abstract：:Alphavirus replicons are very useful for analyzing different aspects of viral molecular biology. They are also useful tools in the development of new vaccines and highly efficient expression of heterologous genes. We have investigated the translatability of Sindbis virus (SV) subgenomic mRNA bearing different 5'-untra...

journal_title：Journal of molecular biology

authors： Sanz MA,Welnowska E,Redondo N,Carrasco L

更新日期：2010-09-10 00:00:00

abstract：:A protein-protein Förster resonance energy transfer (FRET) system, employing probes at multiple positions, was designed to specifically monitor the dissociation of the H2A-H2B dimer from the nucleosome core particle (NCP). Tryptophan donors and Cys-AEDANS acceptors were chosen because, compared to previous NCP FRET fl...

journal_title：Journal of molecular biology

authors： Hoch DA,Stratton JJ,Gloss LM

更新日期：2007-08-24 00:00:00

abstract：:GDP liganded tubulin, which is inactive in microtubule assembly, polymerizes into rings more readily than the active GTP liganded protein. The structure of double rings made of highly purified GDP-tubulin has been characterized to 3 nm resolution with synchrotron X-ray solution scattering. The scattering profile has c...

journal_title：Journal of molecular biology

authors： Díaz JF,Pantos E,Bordas J,Andreu JM

更新日期：1994-04-29 00:00:00

abstract：:The Spo0A protein of Bacillus subtilis is a transcriptional regulator that shows extensive homology to the regulator proteins in bacterial two-component regulatory systems. Phosphorylation of Spo0A is absolutely necessary for the initiation of sporulation. We now show that phospho-Spo0A is a dimer, binds specifically ...

journal_title：Journal of molecular biology

authors： Asayama M,Yamamoto A,Kobayashi Y

更新日期：1995-06-30 00:00:00

abstract：:Recent experimental results suggest that the native fold, or topology, plays a primary role in determining the structure of the transition state ensemble, at least for small, fast-folding proteins. To investigate the extent of the topological control of the folding process, we studied the folding of simplified models ...

journal_title：Journal of molecular biology

authors： Clementi C,Nymeyer H,Onuchic JN

更新日期：2000-05-19 00:00:00

abstract：:The human alternative splicing factor ASF/SF2, an SR (serine-arginine-rich) protein involved in mRNA splicing control, is activated by the multisite phosphorylation of its C-terminal RS domain, a segment containing numerous arginine-serine dipeptide repeats. The protein kinase responsible for this modification, SR-spe...

journal_title：Journal of molecular biology

authors： Ma CT,Velazquez-Dones A,Hagopian JC,Ghosh G,Fu XD,Adams JA

更新日期：2008-02-08 00:00:00

abstract：:The origin recognition complex (ORC), a possible initiator of chromosomal DNA replication in eukaryotes, binds to ATP through its subunits Orc1p and Orc5p. Orc1p possesses ATPase activity. As for DnaA, the Escherichia coli initiator, the ATP-DnaA complex is active but the ADP-DnaA complex is inactive for DNA replicati...

journal_title：Journal of molecular biology

authors： Takenaka H,Makise M,Kuwae W,Takahashi N,Tsuchiya T,Mizushima T

更新日期：2004-06-25 00:00:00

abstract：:Several experimental data support the notion that the recognition of DNA crossovers play an important role in the multiple functions of topoisomerase II. Here, a theoretical analysis of the possible modes of assembly of yeast topoisomerase II with right and left-handed tight DNA crossovers is performed, using the crys...

journal_title：Journal of molecular biology

authors： Timsit Y,Duplantier B,Jannink G,Sikorav JL

更新日期：1998-12-18 00:00:00

abstract：:The protein kinase threonine tyrosine kinase (TTK; also known as Mps1) is a critical component of the spindle assembly checkpoint and a promising drug target for the treatment of aggressive cancers, such as triple negative breast cancer. While the first TTK inhibitors have entered clinical trials, little is known abou...

journal_title：Journal of molecular biology

authors： Uitdehaag JCM,de Man J,Willemsen-Seegers N,Prinsen MBW,Libouban MAA,Sterrenburg JG,de Wit JJP,de Vetter JRF,de Roos JADM,Buijsman RC,Zaman GJR

更新日期：2017-07-07 00:00:00

abstract：:Mature TGF-beta isoforms, which are covalent dimers, signal by binding to three types of cell surface receptors, the type I, II and III TGF-beta receptors. A complex composed of the TGF-beta ligand and the type I and II receptors is required for signaling. The type II receptor is responsible for recruiting TGF-beta in...

journal_title：Journal of molecular biology

authors： De Crescenzo G,Pham PL,Durocher Y,O'Connor-McCourt MD

更新日期：2003-05-16 00:00:00

abstract：:Gene duplication is a common evolutionary process that leads to the expansion and functional diversification of protein subfamilies. The evolutionary events that cause paralogous proteins to bind different protein ligands (functionally diverged interfaces) are investigated and compared to paralogous proteins that bind...

journal_title：Journal of molecular biology

authors： Aiello D,Caffrey DR

更新日期：2012-10-19 00:00:00

abstract：:Previously we have shown that nuclear extracts from mouse cells contain a heterogeneous group of polypeptides (p65, p80, p90, p100) which form distinct DNA-protein complexes on the 18 base-pair sequence element (termed Sal-box), which constitutes the murine rDNA transcription termination signal. These distinct protein...

journal_title：Journal of molecular biology

authors： Smid A,Finsterer M,Grummt I

更新日期：1992-10-05 00:00:00

abstract：:The stability of membrane proteins differs from globular proteins due to the presence of nonpolar membrane-spanning regions. Using a dataset of 929 membrane protein mutations whose effects on thermal stability (ΔTm) were experimentally determined, we found that the average ΔTm due to 190 stabilizing and 232 destabiliz...

journal_title：Journal of molecular biology

authors： Kulandaisamy A,Zaucha J,Frishman D,Gromiha MM

更新日期：2020-09-10 00:00:00

abstract：:dCMP-deaminase-deficient V79/dC hamster cells have highly imbalanced deoxyribonucleoside triphosphate (dNTP) pools, i.e. a 17-fold larger dCTP pool, a slightly reduced dTTP and a very low dGTP pool, compared to dCMP-deaminase-proficient V79/p cells. Nevertheless, the two lines showed the same rates of spontaneous muta...

journal_title：Journal of molecular biology

authors： Darè E,Zhang LH,Jenssen D,Bianchi V

更新日期：1995-10-06 00:00:00

abstract：:We have analysed antigen-contacting residues and combining site shape in the antibody Fv and Fab crystal structures now available from the Protein Data Bank. Antigen-contacting propensities are presented for each antibody residue, allowing a new definition for the complementarity determining regions (CDRs) to be propo...

journal_title：Journal of molecular biology

authors： MacCallum RM,Martin AC,Thornton JM

更新日期：1996-10-11 00:00:00

abstract：:Atomic contact potentials are derived by statistical analysis of atomic surface contact areas versus atom type in a database of non-homologous protein structures. The atomic environment is characterized by the surface area accessible to solvent and the surface of contacts with polar and non-polar atoms. Four types of ...

journal_title：Journal of molecular biology

authors： Delarue M,Koehl P

更新日期：1995-06-09 00:00:00

abstract：:Four new crystal forms of the anti-T lectin from jackfruit (Artocarpus integrifolia) have been prepared and characterized. Three of them, two monoclinic (P21, a = 59.4 A, b = 83.3 A, c = 63.5 A, beta = 107.7 degrees; C2, a = 106.1 A, b = 53.9 A, c = 128.0 A, beta = 95.0 A) and one orthorhombic (C222(1), a = 98.1 A, b ...

journal_title：Journal of molecular biology

authors： Banerjee R,Dhanaraj V,Mahanta SK,Surolia A,Vijayan M

更新日期：1991-10-05 00:00:00

abstract：:The histone N tails correspond to conserved amino acid sequences that are peripherally located in the nucleosome and undergo a variety of post-synthetic modifications during cell cycle. These N tails have been recently recognized as directly interacting with transcription-related proteins. We show here, based on circu...

journal_title：Journal of molecular biology

authors： Banères JL,Martin A,Parello J

更新日期：1997-10-31 00:00:00

abstract：:The three-dimensional structure of barley malt alpha-amylase (isoform AMY2-2) was determined by multiple isomorphous replacement using three heavy-atom derivatives and solvent flattening. The model was refined using a combination of simulated annealing and conventional restrained least-squares crystallographic refinem...

journal_title：Journal of molecular biology

authors： Kadziola A,Abe J,Svensson B,Haser R

更新日期：1994-05-27 00:00:00

abstract：:We report a new vector, pEVOL, for the incorporation of unnatural amino acids into proteins in Escherichia coli using evolved Methanocaldococcus jannaschii aminoacyl-tRNA synthetase(s) (aaRS)/suppressor tRNA pairs. This new system affords higher yields of mutant proteins through the use of both constitutive and induci...

journal_title：Journal of molecular biology

authors： Young TS,Ahmad I,Yin JA,Schultz PG

更新日期：2010-01-15 00:00:00

abstract：:Drosophila development is regulated by two hormones, 20-hydroxyecdysone (ecdysone) and juvenile hormone. We previously found that expression of the E75 gene is induced by both hormones in cultured S2 cells. E75 occupies over 100 kb of genomic DNA; it has four alternative promoters producing isoforms E75A, E75B, E75C, ...

journal_title：Journal of molecular biology

authors： Bernardo TJ,Dubrovskaya VA,Jannat H,Maughan B,Dubrovsky EB

更新日期：2009-04-10 00:00:00

abstract：:Pathogenic Vibrio cholerae secrete V. cholerae cytolysin (VCC), an 80 kDa pro-toxin that assembles into an oligomeric pore on target cell membranes following proteolytic cleavage and interaction with cell surface receptors. To gain insight into the activation and targeting activities of VCC, we solved the crystal stru...

journal_title：Journal of molecular biology

authors： Olson R,Gouaux E

更新日期：2005-07-29 00:00:00

abstract：:We investigate the conservation of amino acid residue sequences in 21 DNA-binding protein families and study the effects that mutations have on DNA-sequence recognition. The observations are best understood by assigning each protein family to one of three classes: (i) non-specific, where binding is independent of DNA ...

journal_title：Journal of molecular biology

authors： Luscombe NM,Thornton JM

更新日期：2002-07-26 00:00:00

abstract：:Normal type I collagen is a heterotrimer of two alpha1(I) and one alpha2(I) chains, but various genetic and environmental factors result in synthesis of homotrimers that consist of three alpha1(I) chains. The homotrimers completely replace the heterotrimers only in rare recessive disorders. In the general population, ...

journal_title：Journal of molecular biology

authors： Han S,McBride DJ,Losert W,Leikin S

更新日期：2008-10-31 00:00:00

abstract：:The nucleotide sequences of nine genes corresponding to tRNA(Ser)4 or tRNA(Ser)7 of Drosophila melanogaster were determined. Eight of the genes compose the major tRNA(Ser)4,7 cluster at 12DE on the X chromosome, while the other is from 23E on the left arm of chromosome 2. Among the eight X-linked genes, five different...

journal_title：Journal of molecular biology

authors： Cribbs DL,Leung J,Newton CH,Hayashi S,Miller RC Jr,Tener GM

更新日期：1987-10-05 00:00:00

abstract：:Ca2+ concentration jumps for the activation of Ca2+-dependent ion channels or transporters can be obtained either by fast solution exchange or by the use of caged Ca2+. Here, we report on an alternate optogenetic method for the activation of Ca2+ and voltage-dependent large conductance (BK) potassium channels. This wa...

journal_title：Journal of molecular biology

authors： Mager T,Wood PG,Bamberg E

更新日期：2017-03-24 00:00:00

abstract：:Aligned sequences from the same family (e.g. the haemoglobins) are seldom representative of the entire family. This is because (1) the sequence databases are heavily skewed toward a small number of organisms and (2) only a minute fraction of all the different family members have been sequenced. For many applications, ...

journal_title：Journal of molecular biology

authors： Sibbald PR,Argos P

更新日期：1990-12-20 00:00:00