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Dimer form of phosphorylated Spo0A, a transcriptional regulator, stimulates the spo0F transcription at the initiation of sporulation in Bacillus subtilis.

Abstract：

:The Spo0A protein of Bacillus subtilis is a transcriptional regulator that shows extensive homology to the regulator proteins in bacterial two-component regulatory systems. Phosphorylation of Spo0A is absolutely necessary for the initiation of sporulation. We now show that phospho-Spo0A is a dimer, binds specifically to the spo0F promoter region, and stimulates the transcription from the P2 promoter recognized by sigma H-RNA polymerase. Biochemical and biological analyses suggest that phospho-Spo0A interacts directly with the "0A-like box" sequence (TGTCGTA) located in the spo0F promoter region. Phosphorylation of Spo0A enhanced its affinity to the 0A-like box. Evidence is also presented that the spo0F promoter region contains a static bend having two sets of oligo(dA-dT) tracts. It was demonstrated that the bending region overlaps with the recognition site for the phospho-Spo0A.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Asayama M,Yamamoto A,Kobayashi Y

doi

10.1006/jmbi.1995.0354

subject

Has Abstract

pub_date

1995-06-30 00:00:00

pages

11-23

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(85)70354-3

journal_volume

250

pub_type

杂志文章

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