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The antiterminator RNA of phage HK022.

Abstract：

:The put sites of phage HK022 increase the processivity of transcription and thereby promote the expression of viral genes that are located downstream of transcription terminators. RNA polymerase molecules that have traversed a put site are converted to a terminator-resistant form by the put transcript. We analyzed the structure and function of put transcripts by determining the effects of put mutations on terminator read-through, and by probing wild-type and mutant put RNAs with structure-specific nucleases. The results support the prediction that the secondary structure of the active transcript consists of two hairpin stems that are separated by a single unpaired base. The identity of bases in certain bulges and internal loops is important for activity, while that of most bases in the terminal loops is not. Many bases in the stems can be replaced with little or no effect on activity provided that base-pairing is maintained.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Banik-Maiti S,King RA,Weisberg RA

doi

10.1006/jmbi.1997.1251

subject

Has Abstract

pub_date

1997-10-10 00:00:00

pages

677-87

issue

5

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(97)91251-1

journal_volume

272

pub_type

杂志文章

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