Crystallization and preliminary X-ray diffraction studies of recombinant human interleukin-5.

abstract：:In both prokaryotic and eukaryotic organisms, repressors and activators are responsible for regulating gene expression. The lac operon is a paradigm for understanding how metabolites function as signaling molecules and modulate transcription. These metabolites or allosteric effector molecules bind to the repressor and...

journal_title：Journal of molecular biology

authors： Daber R,Sharp K,Lewis M

更新日期：2009-10-09 00:00:00

abstract：:The first naturally occurring split intein was found in the dnaE gene of Synechocystis sp. PCC6803 and belongs to a subclass of inteins without a penultimate histidine residue. We describe two high-resolution crystal structures, one derived from an excised Ssp DnaE intein and the second from a splicing-deficient precu...

journal_title：Journal of molecular biology

authors： Sun P,Ye S,Ferrandon S,Evans TC,Xu MQ,Rao Z

更新日期：2005-11-11 00:00:00

abstract：:It is generally believed that DNA replication in most eukaryotes proceeds according to a precise program in which there is a defined temporal order by which each chromosomal region is duplicated. However, the regularity of this program at the level of individual chromosomes, in terms of both the relative timing and th...

journal_title：Journal of molecular biology

authors： Czajkowsky DM,Liu J,Hamlin JL,Shao Z

更新日期：2008-01-04 00:00:00

abstract：:S100P is a small calcium-binding protein of the S100 EF-hand-containing family of proteins. Elevated levels of its mRNA are reported to be associated with the progression to hormone independence and metastasis of prostate cancer and to be associated with loss of senescence in human breast epithelial cells in vitro. Th...

journal_title：Journal of molecular biology

authors： Zhang H,Wang G,Ding Y,Wang Z,Barraclough R,Rudland PS,Fernig DG,Rao Z

更新日期：2003-01-24 00:00:00

abstract：:The low-angle X-ray diffraction pattern from Lethocerus flight muscle fibres was recorded in rigor or under two conditions that modify crossbridge structure and behaviour, aqueous adenylylimidodiphosphate (AMPPNP) and AMPPNP + calcium in an ethylene glycol-water mixture. The effects on the 38.7 nm layer-line peaks (hk...

journal_title：Journal of molecular biology

authors： Tregear RT,Wakabayashi K,Tanaka H,Iwamoto H,Reedy MC,Reedy MK,Sugi H,Amemiya Y

更新日期：1990-07-05 00:00:00

abstract：:Advancements in sequencing in the past decades enabled not only the determination of the human proteome but also the identification of a large number of genetic variations in the human population. The phenotypic effects of these mutations range from neutral for polymorphisms to severe for some somatic mutations. Disea...

journal_title：Journal of molecular biology

authors： Dobson L,Mészáros B,Tusnády GE

更新日期：2018-12-07 00:00:00

abstract：:Using cross-linking coupled to matrix-assisted laser desorption/ionization mass spectrometry and CLIP-Seq sequencing, we determined the peptide and oligonucleotide sequences at the interfaces between the capsid proteins and the genomic RNA of bacteriophage MS2. The results suggest that the same coat protein (CP)-RNA a...

journal_title：Journal of molecular biology

authors： Rolfsson Ó,Middleton S,Manfield IW,White SJ,Fan B,Vaughan R,Ranson NA,Dykeman E,Twarock R,Ford J,Kao CC,Stockley PG

更新日期：2016-01-29 00:00:00

abstract：:Biogenesis of β-barrel membrane proteins is a complex, multistep, and as yet incompletely characterized process. The bacterial porin family is perhaps the best-studied protein family among β-barrel membrane proteins that allows diffusion of small solutes across the bacterial outer membrane. In this study, we have iden...

journal_title：Journal of molecular biology

authors： Naveed H,Jimenez-Morales D,Tian J,Pasupuleti V,Kenney LJ,Liang J

更新日期：2012-05-25 00:00:00

abstract：:A manuscript on allostery signed by Francis Crick and Jeffries Wyman was sent by Crick to Jacques Monod in 1965. Monod transmitted a copy of the manuscript, upon which he had written several comments, to Jean-Pierre Changeux, then a post-doctoral fellow at the University of California Berkeley in the laboratory of How...

journal_title：Journal of molecular biology

authors： Crick FH,Wyman J

更新日期：2013-05-13 00:00:00

abstract：:Contradictory reports in the literature have emphasised either the sequence of small interfering RNAs (siRNA) or the structure of their target molecules to be the major determinant of the efficiency of RNA interference (RNAi) approaches. In the present study, we analyse systematically the contributions of these parame...

journal_title：Journal of molecular biology

authors： Schubert S,Grünweller A,Erdmann VA,Kurreck J

更新日期：2005-05-13 00:00:00

abstract：:The N-terminal segment (FR-H1) of the heavy chain (V(H)) of antibodies shows significant conformational variability correlating with the nature of the amino acids H6, H7 and H10 (Kabat H9). In this study, we have established a causal relationship between the local sequence and the structure of this framework region an...

journal_title：Journal of molecular biology

authors： Jung S,Spinelli S,Schimmele B,Honegger A,Pugliese L,Cambillau C,Plückthun A

更新日期：2001-06-08 00:00:00

abstract：:The temperature dependence of processing of precursor tRNA(Gly) (ptRNA(Gly)) variants carrying a single 2'-OCH(3) or locked nucleic acid (LNA) modification at G+1 by Escherichia coli endoribonuclease P RNA was studied at rate-limiting chemistry. We show, for the first time, that these ribose modifications at nucleotid...

journal_title：Journal of molecular biology

authors： Cuzic S,Heidemann KA,Wöhnert J,Hartmann RK

更新日期：2008-05-23 00:00:00

abstract：:The structure of active site carboxymethylated D-glyceraldehyde-3-phosphate dehydrogenase from Palinurus versicolor was determined in the presence of coenzyme NAD+ at 1.88 A resolution with a final R-factor of 0.175. The structure refinement was carried out on the basis of the structure of holo-GAPDH at 2.0 A resoluti...

journal_title：Journal of molecular biology

authors： Song SY,Xu YB,Lin ZJ,Tsou CL

更新日期：1999-04-09 00:00:00

abstract：:Mutations in the human TAZ gene are associated with Barth Syndrome, an often fatal X-linked disorder that presents with cardiomyopathy and neutropenia. The TAZ gene encodes Tafazzin, a putative phospholipid acyltranferase that is involved in the remodeling of cardiolipin, a phospholipid unique to the inner mitochondri...

journal_title：Journal of molecular biology

authors： McKenzie M,Lazarou M,Thorburn DR,Ryan MT

更新日期：2006-08-18 00:00:00

abstract：:The crystal and molecular structure of a dextran hydrate has been determined through combined electron and X-ray diffraction analysis, aided by stereochemical model refinement. A total of 65 hk0 electron diffraction intensities were measured on frozen single crystals held at the temperature of liquid nitrogen, to a re...

journal_title：Journal of molecular biology

authors： Guizard C,Chanzy H,Sarko A

更新日期：1985-06-05 00:00:00

abstract：:The transcriptional repressor CopR is one of the two copy-number control components of plasmid pIP501. CopR binds as a dimer at two consecutive major grooves on the same face of the DNA. Previously, equilibrium dissociation constants of CopR dimers and the CopR-DNA complex and the intracellular CopR concentration were...

journal_title：Journal of molecular biology

authors： Kuhn K,Steinmetzer K,Brantl S

更新日期：2000-07-28 00:00:00

abstract：:Carbon catabolite repression (CCR) of several operons in Bacillus subtilis and Bacillus megaterium is mediated by the cis-acting cre sequence and trans-acting catabolite control protein (CcpA). We describe purification of CcpA from B. megaterium and its interaction with regulatory sequences from the xyl operon. Specif...

journal_title：Journal of molecular biology

authors： Gösseringer R,Küster E,Galinier A,Deutscher J,Hillen W

更新日期：1997-03-07 00:00:00

abstract：:Mitochondrial respiratory chain complex I undergoes transitions from active to de-activated forms. We have investigated the phenomenon in sub-mitochondrial particles from Neurospora crassa wild-type and a null-mutant lacking the 29.9 kDa nuclear-coded subunit of complex I. Based on enzymatic activities, genetic crosse...

journal_title：Journal of molecular biology

authors： Ushakova AV,Duarte M,Vinogradov AD,Videira A

更新日期：2005-08-12 00:00:00

abstract：:Proteins containing the STAR RNA-binding domain fulfill vital roles in RNA biogenesis, yet a detailed understanding of STAR domain RNA binding specificity is lacking. In Caenorhabditis elegans, the STAR protein GLD-1 directly binds the 28 nucleotide recognition element TGE within the 3' untranslated region of tra-2 mR...

journal_title：Journal of molecular biology

authors： Lehmann-Blount KA,Williamson JR

更新日期：2005-02-11 00:00:00

abstract：:Nuclear transport factor 2 (NTF2) mediates nuclear import of RanGDP, a central component of many nuclear trafficking pathways. NTF2 is a homodimer and each chain has independent binding sites for RanGDP and nuclear pore proteins (nucleoporins) that contain FxFG sequence repeats. We show here that the monomer-dimer dis...

journal_title：Journal of molecular biology

authors： Chaillan-Huntington C,Butler PJ,Huntington JA,Akin D,Feldherr C,Stewart M

更新日期：2001-11-30 00:00:00

abstract：:The structure of Mn(III) superoxide dismutase (Mn(III)SOD) from Thermus thermophilus, a tetramer of chains 203 residues in length, has been refined by restrained least-squares methods. The R-factor [formula: see text] for the 54,056 unique reflections measured between 10.0 and 1.8 A (96% of all possible reflections) i...

journal_title：Journal of molecular biology

authors： Ludwig ML,Metzger AL,Pattridge KA,Stallings WC

更新日期：1991-05-20 00:00:00

abstract：:RNA sites that contain unpaired or mismatched nucleotides can be interaction sites for other macromolecules. C1054, a virtually universally conserved nucleotide in the 16 S (small subunit) ribosomal RNA of Escherichia coli, is part of a highly conserved bulge in helix 34, which has been located at the decoding site of...

journal_title：Journal of molecular biology

authors： Pagel FT,Zhao SQ,Hijazi KA,Murgola EJ

更新日期：1997-04-18 00:00:00

abstract：:The three-dimensional structure of 2H1, a protective monoclonal antibody to Cryptococcus neoformans, has been solved at 2.4 A resolution, in both its unbound form and in complex with the 12 amino acid residue peptide PA1 (GLQYTPSWMLVG). PA1 was previously identified as a potential mimotope of the cryptococcal capsular...

journal_title：Journal of molecular biology

authors： Young AC,Valadon P,Casadevall A,Scharff MD,Sacchettini JC

更新日期：1997-12-12 00:00:00

abstract：:Protein-primed DNA polymerases form a subgroup of the eukaryotic-type DNA polymerases family, also called family B or alpha-like. A multiple amino acid sequence alignment of this subgroup of DNA polymerases led to the identification of two insertions, TPR-1 and TPR-2, in the polymerisation domain. We showed previously...

journal_title：Journal of molecular biology

authors： Dufour E,Rodríguez I,Lázaro JM,de Vega M,Salas M

更新日期：2003-08-22 00:00:00

abstract：:Transposable-element-mediated fusion of the conjugal plasmid pOX38::Tn9 with pBR322 results in the appearance of cointegrates composed of a single copy of each plasmid, and cointegrates which carry a single copy of pOX38 but multiple tandem copies of pBR322. These plasmids are separated by directly repeated copies of ...

journal_title：Journal of molecular biology

authors： Chandler M,Galas DJ

更新日期：1983-03-25 00:00:00

abstract：:We have produced and characterized two new copper-transporting ATPases, CtrA2 and CtrA3 from Aquifex aeolicus, that belong to the family of heavy metal ion-transporting P(IB)-type ATPases. CtrA2 has a CPC metal-binding sequence in TM6 and a CxxC metal-binding N-terminal domain, while CtrA3 has a CPH metal-binding moti...

journal_title：Journal of molecular biology

authors： Chintalapati S,Al Kurdi R,van Scheltinga AC,Kühlbrandt W

更新日期：2008-05-02 00:00:00

abstract：:While the disulfide bridge is highly conserved within the immunoglobulin fold, a few antibody variable domains lack one of the essential cysteine residues. In the levan binding antibody ABPC48 one of the essential cysteine residues (Cys H92) of the heavy chain variable domain is replaced by tyrosine. We expressed scFv...

journal_title：Journal of molecular biology

authors： Proba K,Honegger A,Plückthun A

更新日期：1997-01-17 00:00:00

abstract：:Methyltransferases from the m(1)A(58) tRNA methyltransferase (TrmI) family catalyze the S-adenosyl-l-methionine-dependent N(1)-methylation of tRNA adenosine 58. The crystal structure of Thermus thermophilus TrmI, in complex with S-adenosyl-l-homocysteine, was determined at 1.7 A resolution. This structure is closely r...

journal_title：Journal of molecular biology

authors： Barraud P,Golinelli-Pimpaneau B,Atmanene C,Sanglier S,Van Dorsselaer A,Droogmans L,Dardel F,Tisné C

更新日期：2008-03-21 00:00:00

abstract：:Bacteriorhodopsin is the smallest autonomous light-driven proton pump. Proposals as to how it achieves the directionality of its trans-membrane proton transport fall into two categories: accessibility-switch models in which proton transfer pathways in different parts of the molecule are opened and closed during the ph...

journal_title：Journal of molecular biology

authors： Onufriev A,Smondyrev A,Bashford D

更新日期：2003-10-03 00:00:00

abstract：:Single crystals of the soluble glucose dehydrogenase (GDH) from Acinetobacter calcoaceticus have been grown by the vapour diffusion method. These crystals diffract to beyond 2.1 A and are suitable for X-ray crystallography. The space group was determined to be P2(1) with unit cell parameters a = 55.5 A, b = 104.5 A, c...

journal_title：Journal of molecular biology

authors： Schlunegger MP,Grütter MG,Streiff MB,Olsthoorn AJ,Duine JA

更新日期：1993-10-20 00:00:00