X-ray diffraction and electron microscopy from Lethocerus flight muscle partially relaxed by adenylylimidodiphosphate and ethylene glycol.


:The low-angle X-ray diffraction pattern from Lethocerus flight muscle fibres was recorded in rigor or under two conditions that modify crossbridge structure and behaviour, aqueous adenylylimidodiphosphate (AMPPNP) and AMPPNP + calcium in an ethylene glycol-water mixture. The effects on the 38.7 nm layer-line peaks (hk.6) of the diffraction patterns were studied in detail. In aqueous AMPPNP at room temperature, a condition in which rigor tension drops to half without loss of stiffness, the peaks remained nearly as intense as in rigor except for the 10.6, which dropped to half. In 20% (v/v) ethylene glycol-AMPPNP + 100 microM-Ca2+ at 23 degrees C (gly + pnp + Ca), a condition which removed muscle tension but left stiffness close to the rigor value, the 10.6 and 11.6 peaks greatly decreased but the 31.6 remained relatively high. The 14.5 nm meridional peak (00.16) became stronger on addition of AMPPNP and again on adding glycol + calcium. Considered in terms of constructively interfering filaments and crossbridges, the X-ray data indicated a transfer of diffracting crossbridge mass towards the thick filament as relaxation proceeds. We compared the X-ray diffraction patterns and crossbridge structure seen with electron microscopy (EM) under the same chemical conditions. EM and X-ray observations were mutually quite consistent overall. However, X-ray data indicated that more crossbridge mass was stereospecifically related to actin before fixation in the partially relaxed state (gly + pnp + Ca) than was suggested by the disordered crossbridge profiles seen by EM. We conclude that myosin heads at the start of the power stroke may both be closely related to their thick filament origins and form actin-determined attachments to the thin filament.


J Mol Biol


Tregear RT,Wakabayashi K,Tanaka H,Iwamoto H,Reedy MC,Reedy MK,Sugi H,Amemiya Y




Has Abstract


1990-07-05 00:00:00














  • Solution structure and dynamics of the wild-type pseudoknot of human telomerase RNA.

    abstract::Telomerase is a ribonucleoprotein complex that replicates the 3' ends of linear chromosomes by successive additions of telomere repeat DNA. The telomerase holoenzyme contains two essential components for catalysis, a telomerase reverse transcriptase (TERT) and telomerase RNA (TER). The TER includes a template for telo...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Kim NK,Zhang Q,Zhou J,Theimer CA,Peterson RD,Feigon J

    更新日期:2008-12-31 00:00:00

  • Arrangement of photosystem II supercomplexes in crystalline macrodomains within the thylakoid membrane of green plant chloroplasts.

    abstract::The chloroplast thylakoid membrane of green plants is organized in stacked grana membranes and unstacked stroma membranes. We investigated the structural organization of Photosystem II (PSII) in paired grana membrane fragments by transmission electron microscopy. The membrane fragments were obtained by a short treatme...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Boekema EJ,van Breemen JF,van Roon H,Dekker JP

    更新日期:2000-09-01 00:00:00

  • Cloning, overexpression and purification of the terminase proteins gp16 and gp17 of bacteriophage T4. Construction of a defined in-vitro DNA packaging system using purified terminase proteins.

    abstract::Terminases of double-stranded DNA bacteriophages are required for packaging and generation of terminii in replicated concatemeric DNA molecules. Genetic evidence suggests that these functions in phage T4 are carried out by the products of genes 16 and 17. We cloned these T4 genes into a heat-inducible cI repressor-lam...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Rao VB,Black LW

    更新日期:1988-04-05 00:00:00

  • Monomers of the Escherichia coli SSB-1 mutant protein bind single-stranded DNA.

    abstract::The Escherichia coli wild-type single strand binding (SSB) protein is a stable tetramer that binds to single-stranded (ss) DNA in its role in DNA replication, recombination and repair. The ssb-1 mutation, a substitution of tyrosine for histidine-55 within the SSB-1 protein, destabilizes the tetramer with respect to mo...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Bujalowski W,Lohman TM

    更新日期:1991-01-05 00:00:00

  • Common physical properties of DNA affecting target site selection of sleeping beauty and other Tc1/mariner transposable elements.

    abstract::Sleeping Beauty (SB) is the most active Tc1/mariner-type transposable element in vertebrates, and is therefore a valuable vector for transposon mutagenesis in vertebrate models and for human gene therapy. We have analyzed factors affecting target site selection of SB in mammalian cells, by generating transposition eve...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Vigdal TJ,Kaufman CD,Izsvák Z,Voytas DF,Ivics Z

    更新日期:2002-10-25 00:00:00

  • In human pseudouridine synthase 1 (hPus1), a C-terminal helical insert blocks tRNA from binding in the same orientation as in the Pus1 bacterial homologue TruA, consistent with their different target selectivities.

    abstract::Human pseudouridine (Ψ) synthase Pus1 (hPus1) modifies specific uridine residues in several non-coding RNAs: tRNA, U2 spliceosomal RNA, and steroid receptor activator RNA. We report three structures of the catalytic core domain of hPus1 from two crystal forms, at 1.8Å resolution. The structures are the first of a mamm...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Czudnochowski N,Wang AL,Finer-Moore J,Stroud RM

    更新日期:2013-10-23 00:00:00

  • Nucleosome binding by the bromodomain and PHD finger of the transcriptional cofactor p300.

    abstract::The PHD finger and the bromodomain are small protein domains that occur in many proteins associated with phenomena related to chromatin. The bromodomain has been shown to bind acetylated lysine residues on histone tails. Lysine acetylation is one of several histone modifications that have been proposed to form the bas...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Ragvin A,Valvatne H,Erdal S,Arskog V,Tufteland KR,Breen K,ØYan AM,Eberharter A,Gibson TJ,Becker PB,Aasland R

    更新日期:2004-04-02 00:00:00

  • Crystal structure of the N-terminal dimerisation domain of VicH, the H-NS-like protein of Vibrio cholerae.

    abstract::The histone-like nucleoid structuring (H-NS) protein is a global modulator of gene expression in Gram-negative bacteria. VicH, the H-NS protein of Vibrio cholerae, regulates the expression of certain major virulence determinants implicated in the pathogenesis of cholera. We present here the 2.5A crystal structure of t...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Cerdan R,Bloch V,Yang Y,Bertin P,Dumas C,Rimsky S,Kochoyan M,Arold ST

    更新日期:2003-11-21 00:00:00

  • Topological frustration in beta alpha-repeat proteins: sequence diversity modulates the conserved folding mechanisms of alpha/beta/alpha sandwich proteins.

    abstract::The thermodynamic hypothesis of Anfinsen postulates that structures and stabilities of globular proteins are determined by their amino acid sequences. Chain topology, however, is known to influence the folding reaction, in that motifs with a preponderance of local interactions typically fold more rapidly than those wi...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Hills RD Jr,Kathuria SV,Wallace LA,Day IJ,Brooks CL 3rd,Matthews CR

    更新日期:2010-04-30 00:00:00

  • A novel allosteric mechanism in haemoglobin. Structure of bovine deoxyhaemoglobin, absence of specific chloride-binding sites and origin of the chloride-linked Bohr effect in bovine and human haemoglobin.

    abstract::The structure of bovine deoxyhaemoglobin has been determined at 2.2 A resolution and refined to an R-factor of 0.193 for all 32,583 reflections, and a free R-factor of 0.249 for 1527 reflections excluded from the refinement. The structure shows no significant differences between the alpha-carbon positions of bovine an...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Perutz MF,Fermi G,Poyart C,Pagnier J,Kister J

    更新日期:1993-10-05 00:00:00

  • Amino acid sequence, crystallization and structure determination of reduced and oxidized cytochrome c6 from the green alga Scenedesmus obliquus.

    abstract::Cytochrome c6from the unicellular green alga Scenedesmus obliquus was sequenced, crystallized in its reduced and oxidized state and the three-dimensional structure of the protein in both redox states was determined by X-ray crystallography. Reduced cytochrome c6crystallized as a monomer in the space group P 21212, whe...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Schnackenberg J,Than ME,Mann K,Wiegand G,Huber R,Reuter W

    更新日期:1999-07-30 00:00:00

  • ABCF ATPases Involved in Protein Synthesis, Ribosome Assembly and Antibiotic Resistance: Structural and Functional Diversification across the Tree of Life.

    abstract::Within the larger ABC superfamily of ATPases, ABCF family members eEF3 in Saccharomyces cerevisiae and EttA in Escherichia coli have been found to function as ribosomal translation factors. Several other ABCFs including biochemically characterized VgaA, LsaA and MsrE confer resistance to antibiotics that target the pe...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Murina V,Kasari M,Takada H,Hinnu M,Saha CK,Grimshaw JW,Seki T,Reith M,Putrinš M,Tenson T,Strahl H,Hauryliuk V,Atkinson GC

    更新日期:2019-08-23 00:00:00

  • ATP synthase from bovine heart mitochondria. In vitro assembly of a stalk complex in the presence of F1-ATPase and in its absence.

    abstract::Four subunits of the F1F0-ATPase from bovine heart mitochondria have been produced by heterologous over-expression in Escherichia coli. They are the oligomycin sensitivity conferral protein (OSCP), coupling factor 6 (F6) and subunits b and d. Likewise, fragments b', bI, bC, and bM (amino acid residues 79 to 214, 121 t...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Collinson IR,van Raaij MJ,Runswick MJ,Fearnley IM,Skehel JM,Orriss GL,Miroux B,Walker JE

    更新日期:1994-09-30 00:00:00

  • The Bacterial Transcription Termination Factor Rho Coordinates Mg(2+) Homeostasis with Translational Signals.

    abstract::The bacterial protein Rho triggers transcription termination at the ends of many operons and when transcription and translation become uncoupled. In addition to these genome wide activities, Rho implements regulation of specific genes by dictating whether RNA polymerase terminates transcription within the 5' leader re...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Kriner MA,Groisman EA

    更新日期:2015-12-04 00:00:00

  • Multi-timescale dynamics study of FKBP12 along the rapamycin-mTOR binding coordinate.

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Sapienza PJ,Mauldin RV,Lee AL

    更新日期:2011-01-14 00:00:00

  • Structural characterization of protein-protein complexes by integrating computational docking with small-angle scattering data.

    abstract::X-ray crystallography and NMR can provide detailed structural information of protein-protein complexes, but technical problems make their application challenging in the high-throughput regime. Other methods such as small-angle X-ray scattering (SAXS) are more promising for large-scale application, but at the cost of l...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Pons C,D'Abramo M,Svergun DI,Orozco M,Bernadó P,Fernández-Recio J

    更新日期:2010-10-22 00:00:00

  • Multiple DNA conformational changes induced by an initiator protein precede the nicking reaction in a rolling circle replication origin.

    abstract::The core origin for plus strand DNA replication of filamentous bacteriophage f1 binds the initiator protein (gpII), which subsequently introduces a specific nick in the plus strand. The core origin consists of a nicking region and a binding region. The binding of gpII occurs in two steps, forming a binding intermediat...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Higashitani A,Greenstein D,Hirokawa H,Asano S,Horiuchi K

    更新日期:1994-04-08 00:00:00

  • The regions of the sequence most exposed to the solvent within the amyloidogenic state of a protein initiate the aggregation process.

    abstract::Formation of misfolded aggregates is an essential part of what proteins can do. The process of protein aggregation is central to many human diseases and any aggregating event needs to be prevented within a cell and in protein design. In order to aggregate, a protein needs to unfold its native state, at least partially...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Monti M,Garolla di Bard BL,Calloni G,Chiti F,Amoresano A,Ramponi G,Pucci P

    更新日期:2004-02-06 00:00:00

  • Yeast amber suppressors corresponding to tRNA3Leu genes.

    abstract::Amber suppressors previously isolated from the yeast Saccharomyces cerevisiae and belonging to the same phenotypic class (Liebman et al., 1976) were assigned to nine different linkage groups named SUP52 through SUP60. One of these suppressors, SUP52, had been shown to cause the insertion of leucine and had been geneti...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Liebman SW,Srodulski Z,Reed CR,Stewart JW,Sherman F,Brennan G

    更新日期:1984-09-15 00:00:00

  • Optogenetic Control of Ca2+ and Voltage-Dependent Large Conductance (BK) Potassium Channels.

    abstract::Ca2+ concentration jumps for the activation of Ca2+-dependent ion channels or transporters can be obtained either by fast solution exchange or by the use of caged Ca2+. Here, we report on an alternate optogenetic method for the activation of Ca2+ and voltage-dependent large conductance (BK) potassium channels. This wa...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Mager T,Wood PG,Bamberg E

    更新日期:2017-03-24 00:00:00

  • Negative cooperativity of uric acid binding to the transcriptional regulator HucR from Deinococcus radiodurans.

    abstract::Members of the MarR family of winged helix transcriptional regulators have been shown to regulate multidrug and oxidative stress response, pathogenesis, and catabolism of aromatic compounds. Many respond to anionic lipophilic compounds in their capacity to bind DNA, and the co-crystal structure of MarR bound to salicy...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Wilkinson SP,Grove A

    更新日期:2005-07-22 00:00:00

  • Negative Epistasis and Evolvability in TEM-1 β-Lactamase--The Thin Line between an Enzyme's Conformational Freedom and Disorder.

    abstract::Epistasis is a key factor in evolution since it determines which combinations of mutations provide adaptive solutions and which mutational pathways toward these solutions are accessible by natural selection. There is growing evidence for the pervasiveness of sign epistasis--a complete reversion of mutational effects, ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Dellus-Gur E,Elias M,Caselli E,Prati F,Salverda ML,de Visser JA,Fraser JS,Tawfik DS

    更新日期:2015-07-17 00:00:00

  • Changes in the molecular packing of fibrillin microfibrils during extension indicate intrafibrillar and interfibrillar reorganization in elastic response.

    abstract::Fibrillin-rich microfibrils are the major structural components of the extracellular matrix that provide elasticity in a majority of connective tissues. The basis of elastic properties lies in the organization of fibrillin molecules, which, unfortunately, is still poorly understood. An X-ray diffraction study of hydra...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Glab J,Wess T

    更新日期:2008-11-28 00:00:00

  • The activation defect of a lambda cI positive control mutant.

    abstract::"Positive control" mutants of the cI protein of bacteriophage lambda (lambda cI) bind DNA but, unlike the wild-type protein, fail to activate transcription. According to the original interpretation of Ptashne and co-workers, these mutants bear amino acid substitutions that disrupt a stimulatory interaction between lam...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Whipple FW,Ptashne M,Hochschild A

    更新日期:1997-01-24 00:00:00

  • Titration calculations of foot-and-mouth disease virus capsids and their stabilities as a function of pH.

    abstract::Foot-and-mouth disease virus (FMDV), a non-enveloped picornavirus, is sensitive to acidic conditions. At pH values below 7 the icosahedral virus capsid, formed from 60 copies of a protomer containing four polypeptides (VP1 to 4), dissociates into 12 pentamers, releasing the viral RNA. Evidence suggests that this acid ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: van Vlijmen HW,Curry S,Schaefer M,Karplus M

    更新日期:1998-01-16 00:00:00

  • Crystal Structures of Ternary Complexes of MEF2 and NKX2-5 Bound to DNA Reveal a Disease Related Protein-Protein Interaction Interface.

    abstract::MEF2 and NKX2-5 transcription factors interact with each other in cardiogenesis and are necessary for normal heart formation. Despite evidence suggesting that these two transcription factors function synergistically and possibly through direct physical interactions, molecular mechanisms by which they interact are not ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Lei X,Zhao J,Sagendorf JM,Rajashekar N,Xu J,Dantas Machado AC,Sen C,Rohs R,Feng P,Chen L

    更新日期:2020-09-04 00:00:00

  • Temperature-dependent dynamics of the villin headpiece helical subdomain, an unusually small thermostable protein.

    abstract::(15)N spin relaxation experiments were used to measure the temperature-dependence of protein backbone conformational fluctuations in the thermostable helical subdomain, HP36, of the F-actin-binding headpiece domain of chicken villin. HP36 is the smallest domain of a naturally occurring protein that folds cooperatively...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Vugmeyster L,Trott O,McKnight CJ,Raleigh DP,Palmer AG 3rd

    更新日期:2002-07-19 00:00:00

  • Co-axial association of recombinant eye lens aquaporin-0 observed in loosely packed 3D crystals.

    abstract::Aquaporin-0 (AQP0) is the major membrane protein in vertebrate eye lenses. It has been proposed that AQP0 tetramers mediate contact between membranes of adjacent lens fiber cells, which would be consistent with the extraordinarily narrow inter-cellular spacing. We have obtained 3D crystals of recombinant bovine AQP0 t...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Palanivelu DV,Kozono DE,Engel A,Suda K,Lustig A,Agre P,Schirmer T

    更新日期:2006-01-27 00:00:00

  • Structure-guided identification of a laminin binding site on the laminin receptor precursor.

    abstract::The 37/ 67-kDa human laminin receptor (LamR) is a cell surface receptor for laminin, prion protein, and a variety of viruses. Because of its wide range of ligands, LamR plays a role in numerous pathologies. LamR overexpression correlates with a highly invasive cell phenotype and increased metastatic ability, mediated ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章,评审


    authors: Jamieson KV,Hubbard SR,Meruelo D

    更新日期:2011-01-07 00:00:00

  • Repacking the Core of T4 lysozyme by automated design.

    abstract::Automated protein redesign, as implemented in the program ORBIT, was used to redesign the core of phage T4 lysozyme. A total of 26 buried or partially buried sites in the C-terminal domain were allowed to vary both their sequence and side-chain conformation while the backbone and non-selected side-chains remained fixe...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Mooers BH,Datta D,Baase WA,Zollars ES,Mayo SL,Matthews BW

    更新日期:2003-09-19 00:00:00