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High affinity of endonuclease VII for the Holliday structure containing one nick ensures productive resolution.

Abstract：

:During homologous recombination, genetic information is physically exchanged between parental DNAs via crossing single strands of the same polarity within a four-way DNA junction called a Holliday structure. This process is terminated by the endonucleolytic activity of resolvases, which convert the four-way DNA back to two double strands. To achieve productive resolution, the two subunits of the dimeric enzymes introduce two single-strand cuts positioned symmetrically in opposite strands across the DNA junction. Covalently linked dimers of endonuclease VII from phage T4, whether a homodimer with two or a heterodimer with only one functional catalytic centre, reacted with a synthetic cruciform DNA to form a DNA-enzyme complex immediately after addition of the enzyme. Analysis of the complexes from both reactions revealed that the bound junction contained one nick. While the active homodimer processed this nicked junction consecutively to duplex DNAs by making the second cut, the complex with the heterodimer stayed stable for the whole reaction time. Thus the high affinity of endonuclease VII for the junction containing one nick is part of the mechanism to ensure productive resolution of Holliday structures, by giving the enzyme time to make the second cut, whereupon the complex dissociates into the two duplex DNAs and the free enzyme.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Birkenbihl RP,Kemper B

doi

10.1016/s0022-2836(02)00594-6

keywords:

subject

Has Abstract

pub_date

2002-08-02 00:00:00

pages

21-8

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022283602005946

journal_volume

321

pub_type

杂志文章

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