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The three-dimensional arcitecture of the EJC core.

Abstract：

:The exon junction complex (EJC) is a macromolecular complex deposited at splice junctions on mRNAs as a consequence of splicing. At the core of the EJC are four proteins: eIF4AIII, a member of the DExH/D-box family of NTP-dependent RNA binding proteins, Y14, Magoh, and MLN51. These proteins form a stable heterotetramer that remains bound to the mRNA throughout many different cellular environments. We have determined the three-dimensional (3D) structure of this EJC core using negative-stain random-conical tilt electron microscopy. This structure represents the first structure of a DExH/D-box protein in complex with its binding partners. The EJC core is a four-lobed complex with a central channel and dimensions consistent with its known RNA footprint of about ten nucleotides. Using known X-ray crystallographic structures and a model of three of the four components, we propose a model for complex assembly on RNA and explain how Y14:Magoh may influence eIF4AIII's RNA binding.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Stroupe ME,Tange TØ,Thomas DR,Moore MJ,Grigorieff N

doi

10.1016/j.jmb.2006.05.049

subject

Has Abstract

pub_date

2006-07-21 00:00:00

pages

743-9

issue

4

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(06)00650-4

journal_volume

360

pub_type

杂志文章

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